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Superoxide Formation in Cardiac Mitochondria and Effect of Phenolic Antioxidants

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Abstract

Since mitochondria are the main cellular source of reactive oxygen species, it is important to study the effect of dietary phenolic compounds on the level of ROS in these organelles. Using the EPR spectroscopy and TIRON probe, the ability of the investigated phenols (quercetin, rutin, caffeic acid, curcumin, and resveratrol) to scavenge superoxide anion radicals generated by isolated heart mitochondria of Wistar rats under variable oxygen partial pressure was studied. It was shown that during a 10 min incubation, caffeic acid in concentrations of 10–500 μM most effectively scavenged superoxide radicals formed in the complex III of the mitochondrial respiratory chain. A comparable antioxidant effect of rutin under these experimental conditions was observed at higher concentrations of 1–10 mM. The antioxidant activity of quercetin in the concentration range of 10–500 μM during the first minutes of incubation was higher than that of caffeic acid. Of the phenolic compounds studied, curcumin had the least effect on the superoxide radicals.

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Abbreviations

Caf-OH:

caffeic acid (3,4-dihydroxycinnamic acid)

Cur:

curcumin ((1,7-bis-(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione)

LiPc:

lithium phthalocyanine

LDL:

low density lipoprotein

O2 •− :

superoxide anion radical

Qu:

quercetin (3,3',4',5,7-pentahydroxyflavone)

ROS:

reactive oxygen species

Rsv:

resveratrol (3,5,4'-trihydroxy-trans-stilbene)

Rut:

rutin (vitamin P)

TEMPONE-15N-D16 :

4-oxo-2,2,6,6-tetramethylpiperidine-D16-1-oxyl-15N

TIRON:

4,5-dihydroxybenzene-1,3-disulfonate, disodium salt

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Acknowledgements

This work was supported by the Russian Foundation for Basic Research (grants 15-04-05211 and 18-015-00125).

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Authors and Affiliations

  1. Faculty of Physics, Lomonosov Moscow State University, Moscow, Russia

    Arina L. Dudylina & Enno K. Ruuge

  2. National Medical Research Centre for Cardiology, Moscow, Russia

    Arina L. Dudylina, Marina V. Ivanova, Konstantin B. Shumaev & Enno K. Ruuge

  3. Bach Institute of Biochemistry, Research Centre of Biotechnology, Russian Academy of Sciences, Moscow, Russia

    Konstantin B. Shumaev

Authors
  1. Arina L. Dudylina
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  2. Marina V. Ivanova
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Correspondence to Enno K. Ruuge.

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Dudylina, A.L., Ivanova, M.V., Shumaev, K.B. et al. Superoxide Formation in Cardiac Mitochondria and Effect of Phenolic Antioxidants. Cell Biochem Biophys 77, 99–107 (2019). https://doi.org/10.1007/s12013-018-0857-2

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