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Synthesis of Composites SBA-15 Mesoporous Particles Carrying Oxytocin and Evaluation of Their Properties, Functions, and In Vitro Biological Activities

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Abstract

Using the organic template method, we have synthesized mesoporous SBA-15 particles and characterized them by scanning electron microscopy and transmission electron microscopy. The bone metabolism regulating hormone oxytocin (OT) was selected as a model for preparation of drug/SBA-15 complexes. The process of drug loading was studied using X-ray diffraction and nitrogen absorption methods. Optimal drug loading parameters were experimentally investigated. The kinetics of drug release from the carrier was evaluated. Finally, the extractions of SBA-15 particles were tested for cytotoxicity, in vitro hemolysis, and the direct attachment toxicity. Our findings suggest that SBA-15 materials have good biocompatibility. Moreover, we demonstrated that OT/SBA-15 complex can stimulate alkaline phosphatase activity in osteoblast cells. The study provides fundamental information for further in vivo drug-carrier testing.

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Acknowledgments

This study was supported by Grants from the Department of Science and Technology of Jilin Province (No.20130522036JH) and the Health Department of Jilin Province (No.3D512Y033431).

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Authors and Affiliations

  1. Department of Prosthodontics, School of Stomatology, Jilin University, Changchun, 130021, Jilin, China

    Ming Qian, Mengna Duan & Zhe Wu

  2. Department of Periodontics, School of Stomatology, Jilin University, Changchun, 130021, Jilin, China

    Min Liu

  3. Department of Implantology, School of Stomatology, Jilin University, Changchun, 130021, Jilin, China

    Yanmin Zhou

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  1. Ming Qian
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  2. Min Liu
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  3. Mengna Duan
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  4. Zhe Wu
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  5. Yanmin Zhou
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Correspondence to Yanmin Zhou.

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Qian, M., Liu, M., Duan, M. et al. Synthesis of Composites SBA-15 Mesoporous Particles Carrying Oxytocin and Evaluation of Their Properties, Functions, and In Vitro Biological Activities. Cell Biochem Biophys 71, 127–134 (2015). https://doi.org/10.1007/s12013-014-0172-5

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  • DOI: https://doi.org/10.1007/s12013-014-0172-5

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