This experiment was conducted to investigate the effects of zinc oxide/zeolite on growth performance, serum biochemistry, intestinal morphology, and microflora of weaned piglets. Two hundred and fifty-six weaned piglets (Duroc × Landrace × Large) at 21 days of age were randomly assigned to 2 groups with 8 replicates and 16 piglets in each pen. The diets of high dose of zinc oxide group (HD-ZnO) supplemented with 1500 mg/kg zinc as zinc oxide, but the diet of experimental group supplemented with 500 mg/kg zinc as zinc oxide that supported on zeolite (SR-ZnO). The experiment was conducted for 2 weeks after weanling. The results showed replacement of high-dosed zinc oxide by SR-ZnO had no significant effects on growth performance and intestinal morphology. However, the dietary supplementation of SR-ZnO reduced the diarrhea rate (P < 0.05), increased the activity of serum alkaline phosphatase (ALP) (P < 0.01), and tended to reduce zinc release in stomach (P = 0.06) and increase serum total protein (TP) (P = 0.07). Although there were no significant effects in ileal microflora on α diversity, the abundance of Campylobacters was found significantly decreased (P < 0.05), whereas the abundance of Clostridium was increased (P < 0.05) after lower-dosed SR-ZnO replacement. It is revealed that replacement of HD-ZnO (1500 mg/kg) by SR-ZnO (500 mg/kg) in creep feed could improve the zinc bioavailability, regulate the intestinal flora, and alleviate the postweaning diarrhea in weaned piglets. Accordingly, the application of SR-ZnO would reduce the zinc in feed and therefore benefits for the ecological environment.
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Wijtten PJ, Van Der Meulen J, Verstegen MW (2011) Intestinal barrier function and absorption in pigs after weaning: a review. Br J Nutr 105:967–981. https://doi.org/10.1017/S0007114510005660
Hill G, Mahan D, Carter S, Cromwell G, Ewan R, Harrold R, Lewis A, Miller PS, Shurson GC, Veum T (2001) Effect of pharmacological concentrations of zinc oxide with or without the inclusion of an antibacterial agent on nursery pig performance. J Anim Sci 79:934–941. https://doi.org/10.2527/2001.794934x
Kim J, Hansen CF, Mullan B, Pluske J (2012) Nutrition and pathology of weaner pigs: nutritional strategies to support barrier function in the gastrointestinal tract. Anim Feed Sci Technol 173:3–16. https://doi.org/10.1016/j.anifeedsci.2011.12.022
Cardinal F, D'allaire S, Fairbrother JM (2006) Feed composition in herds with or without postweaning Escherichia coli diarrhea in early-weaned piglets. J Swine Health Prod 14:10–17
Montagne L, Cavaney FS, Hampson DJ, Lalles JP, Pluske JR (2004) Effect of diet composition on postweaning colibacillosis in piglets. J Anim Sci 82:2364–2374
Lena M, Ulrike L, Angelika B, Eva-Maria G, Vahjen W, Aschenbach JR, Zentek J, Pieper R (2013) A high amount of dietary zinc changes the expression of zinc transporters and metallothionein in jejunal epithelial cells in vitro and in vivo but does not prevent zinc accumulation in jejunal tissue of piglets. J Nutr 143:1205–1210. https://doi.org/10.3945/jn.113.177881
Smith JW, Tokach MD, Goodband RD, Nelssen JL, Richert BT (1997) Effects of the interrelationship between zinc oxide and copper sulfate on growth performance of early-weaned pigs. J Anim Sci 75:1861–1866. https://doi.org/10.2527/1997.7571861x
Hu CH, Xiao K, Song J, Luan ZS (2013) Effects of zinc oxide supported on zeolite on growth performance, intestinal microflora and permeability, and cytokines expression of weaned pigs. Anim Feed Sci Technol 181:65–71. https://doi.org/10.1016/j.anifeedsci.2013.02.003
Vahjen W, Pieper R, Zentek J (2011) Increased dietary zinc oxide changes the bacterial core and enterobacterial composition in the ileum of piglets. J Anim Sci 89:2430–2439. https://doi.org/10.2527/jas.2010-3270
Slifierz MJ, Park J, Friendship RM, Weese JS (2014) Zinc-resistance gene CzrC identified in methicillin-resistant Staphylococcus hyicus isolated from pigs with exudative epidermitis. Can Vet J 55:489
Gresse R, Chaucheyras-Durand F, Fleury MA, Van De Wiele T, Forano E, Blanquet-Diot S (2017) Gut microbiota Dysbiosis in postweaning piglets: understanding the keys to health. Trends Microbiol 25:851–873
Jondreville C, Revy PS, Dourmad JY (2003) Dietary means to better control the environmental impact of copper and zinc by pigs from weaning to slaughter. Livest Prod Sci 84:147–156. https://doi.org/10.1016/j.livprodsci.2003.09.011
Wang Y, Tang JW, Ma WQ, Feng J, Feng J (2010) Dietary zinc glycine chelate on growth performance, tissue mineral concentrations, and serum enzyme activity in weanling piglets. Biol Trace Elem Res 133:325–334. https://doi.org/10.1007/s12011-009-8437-3
Wan D, Zhang YM, Wu X, Lin X, Shu XG, Zhou XH, Du HT, Xing WG, Liu HN, Li L, Li Y, Yin YL (2018) Maternal dietary supplementation with ferrous N-carbamylglycinate chelate affects sow reproductive performance and iron status of neonatal piglets. Animal 12:1372–1379
Zhang Y, Wan D, Zhou X, Long C, Wu X, Li L, He L, Huang P, Chen S, Tan B (2017) Diurnal variations in iron concentrations and expression of genes involved in iron absorption and metabolism in pigs. Biochem Biophys Res Commun 490:1210–1214
Zhu C, Lv H, Chen Z, Wang L, Wu X, Chen Z, Zhang W, Liang R, Jiang Z (2017) Dietary zinc oxide modulates antioxidant capacity, small intestine development, and jejunal gene expression in weaned piglets. Biol Trace Elem Res 175:331–338. https://doi.org/10.1007/s12011-016-0767-3
Xiong X, Zhou J, Liu H, Yin Y (2019) Dietary lysozyme supplementation contributes to enhanced intestinal functions and gut microflora of piglets. Food Funct 10:1696–1706. https://doi.org/10.1039/c8fo02335b
Dong Z, Wan D, Li G, Zhang Y, Yang H, Wu X, Yin Y (2019) Comparison of oral and parenteral iron administration on iron homeostasis, oxidative and immune status in anemic neonatal pigs. Biol Trace Elem Res 195:117–124. https://doi.org/10.1007/s12011-019-01846-9
Zhang YM, Zhou J, Dong ZL, Li GY, Wang JJ, Li YK, Wan D, Yang HS, Yin YL (2019) Effect of dietary copper on intestinal microbiota and antimicrobial resistance profiles of Escherichia coli in weaned piglets. Front Microbiol 10:11. https://doi.org/10.3389/fmicb.2019.02808
Zhou J, Xiong X, Wang KX, Zou LJ, Ji P, Yin YL (2018) Ethanolamine enhances intestinal functions by altering gut microbiome and mucosal anti-stress capacity in weaned rats. Br J Nutr 120:241–249
Edgar RC (2013) UPARSE: highly accurate OTU sequences from microbial amplicon reads. Nat Methods 10:996–998. https://doi.org/10.1038/NMETH.2604
Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, Fierer N, Pena AG, Goodrich JK, Gordon JI (2010) QIIME allows analysis of high-throughput community sequencing data. Nat Methods 7:335–336. https://doi.org/10.1038/nmeth.f.303
Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32:1792–1797. https://doi.org/10.1093/nar/gkh340
Team RC (2013) R: a language and environment for statistical computing. Wiley, New York
Zhang B, Guo Y (2009) Supplemental zinc reduced intestinal permeability by enhancing occludin and zonula occludens protein-1 (ZO-1) expression in weaning piglets. Br J Nutr 102:687–693. https://doi.org/10.1017/s0007114509289033
Jensen-Waern M, Melin L, Lindberg R, Johannisson A, Petersson L, Wallgren P (1998) Dietary zinc oxide in weaned pigs-effects on performance, tissue concentrations, morphology, neutrophil functions and faecal microflora. Res Vet Sci 64:225–231. https://doi.org/10.1016/s0034-5288(98)90130-8
Carlson D, Poulsen HD, Vestergaard M (2004) Additional dietary zinc for weaning piglets is associated with elevated concentrations of serum IGF-I. J Anim Physiol Anim Nutr 88:332–339. https://doi.org/10.1111/j.1439-0396.2004.00488.x
Yablanski T (1986) Lordation between the activity of the plasma enzyme GOT, GPT, AKP and some performance qualities in pigs. Col Sci Work 30:599–616. https://doi.org/10.1016/S0033-3506(88)80029-5
Sun J, Jing M, Weng X, Fu L, Xu Z, Zi N, Wang J (2005) Effects of dietary zinc levels on the activities of enzymes, weights of organs, and the concentrations of zinc and copper in growing rats. Biol Trace Elem Res 107:153–165. https://doi.org/10.1385/BTER:107:2:153
Yousef M, El-Hendy H, El-Demerdash F, Elagamy E (2002) Dietary zinc deficiency induced-changes in the activity of enzymes and the levels of free radicals, lipids and protein electrophoretic behavior in growing rats. Toxicology 175:223–234. https://doi.org/10.1016/s0300-483x(02)00049-5
Zhang Y, Ward TL, Ji F, Peng C, Zhu L, Gong L, Dong B (2018) Effects of zinc sources and levels of zinc amino acid complex on growth performance, hematological and biochemical parameters in weanling pigs. Asian Austral J Anim 31:1267–1274. https://doi.org/10.5713/ajas.17.0739
Xu Z, Wang M (2001) Approach of the mechanism of growth promoting effect of pharmacological level of zinc in pigs. Chin J Anim Vet Sci 32:11–17
Wiyaporn M, Thongsong B, Kalandakanond-Thongsong S (2013) Growth and small intestine histomorphology of low and normal birth weight piglets during the early suckling period. Livest Sci 158:215–222. https://doi.org/10.1016/j.livsci.2013.10.016
Bellino C, D'angelo A, Baricco G, Capucchio M, Sapino R, Amedeo S, Bonetto E, Cagnasso A (2008) Effects of high dietary concentration (3,000 ppm) of Zn as zinc oxide on morphology and activity of alimentary system in weaned piglets. Large Anim Rev 14:21–25. https://doi.org/10.1262/jrd.19159
Sargeant HR, Mcdowall KJ, Miller HM, Shaw M-A (2010) Dietary zinc oxide affects the expression of genes associated with inflammation: transcriptome analysis in piglets challenged with ETEC K88. Vet Immunol Immunopathol 137:120–129. https://doi.org/10.1016/j.vetimm.2010.05.001
Li BT, Van AG, Caine WR, Huang SX, Kirkwood RN (2001) Small intestinal morphology and bacterial populations in ileal digesta and feces of newly weaned pigs receiving a high dietary level of zinc oxide. Can J Anim Sci 81:511–516. https://doi.org/10.4141/A01-043
Namkung H, Gong J, Yu H (2006) Effect of pharmacological intakes of zinc and copper on growth performance, circulating cytokines and gut microbiota of newly weaned piglets challenged with coliform lipopolysaccharides. Can J Anim Sci 86:511–522. https://doi.org/10.4141/a05-075
Yu T, Zhu C, Chen S, Gao L, Lv H, Feng R, Zhu Q, Xu J, Chen Z, Jiang Z (2017) Dietary high zinc oxide modulates the microbiome of ileum and colon in weaned piglets. Front Microbiol 8:1–12. https://doi.org/10.3389/fmicb.2017.00825
Xie Y, He Y, Irwin PL, Jin T, Shi X (2011) Antibacterial activity and mechanism of action of zinc oxide nanoparticles against Campylobacter jejuni. Appl Environ Microbiol 77:2325–2331. https://doi.org/10.1128/AEM.02149-10
Samuel BS, Hansen EE, Manchester JK, Coutinho PM, Henrissat B, Fulton R, Latreille P, Kim K, Wilson RK, Gordon JI (2007) Genomic and metabolic adaptations of Methanobrevibacter smithii to the human gut. Proc Natl Acad Sci 104:10643–10648. https://doi.org/10.1073/pnas.0704189104
Tallant TC, Paul L, Krzycki JA (2001) The MtsA subunit of the methylthiol: coenzyme M methyltransferase of Methanosarcina barkeri catalyses both half-reactions of corrinoid-dependent dimethylsulfide: coenzyme M methyl transfer. J Biol Chem 276:4485–4493. https://doi.org/10.1074/jbc.M007514200
Hamann N, Mander GJ, Shokes JE, Scott RA, Bennati M, Hedderich R (2007) A cysteine-rich CCG domain contains a novel [4Fe-4S] cluster binding motif as deduced from studies with subunit B of heterodisulfide reductase from Methanothermobacter marburgensis. Biochemistry-us. 46:12875–12885. https://doi.org/10.1021/bi700679u
This work was supported by the National Key R&D Program of China (2016YFD0501201), the National Natural Science Foundation of China (31702127), the Young Elite Scientists Sponsorship Program by CAST (2018QNRC001), and the Hunan Province Key Laboratory of Animal Nutritional Physiology and Metabolic Process (2018TP1031), The Science and Technology Service Network Initiative program of Chinese Academy of Sciences (KFJ-STS-QYZD-095), and Yangzhou Science and Technology Bureau-Modern Agricultural Technology Project (SNY2017030037).
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Wang, J., Li, C., Yin, Y. et al. Effects of Zinc Oxide/Zeolite on Intestinal Morphology, Intestinal Microflora, and Diarrhea Rates in Weaned Piglets. Biol Trace Elem Res 199, 1405–1413 (2021). https://doi.org/10.1007/s12011-020-02262-0
- Zinc oxide/zeolite
- Weaned piglets
- Intestinal morphology
- Intestinal microflora