The Effect of Different Levels of Dietary Magnesium on Eggshell Quality and Laying Hen’s Performance


This study aimed to investigate the effects of different levels of dietary magnesium (Mg) on eggshell quality and laying hen’s performance. Four dietary treatments including a commercial-type basal diet containing 3.19 g/kg Mg and three additional diets supplemented with 2, 4, or 6 g/kg magnesium oxide (MgO) to provide 4.03, 4.87, or 5.71 g/kg Mg were used in a completely randomized design. A total of 120 Bovans White layers at the age of 35 weeks were fed with dietary treatments for 8 weeks. Results indicated that increasing concentrations of Mg in the diets significantly increased hen-day egg production and feed intake (P < 0.05). Dietary Mg supplementation had no effect on egg weight, feed conversion ratio, and average daily weight gain. Eggshell weight, thickness, and strength were significantly increased by adding Mg to the diets (P < 0.05). Dietary treatments significantly increased yolk weight (P < 0.05) but decreased yolk color (P < 0.05). Albumen weight, Haugh unit, yolk shape, and egg shape index were similar among the dietary treatments. Eggshell Mg content (g/kg of dry matter) was significantly increased by the inclusion of Mg in the diets. In conclusion, the results of this study indicated that supplementation of Mg up to 5.71 g/kg diet improved eggshell quality and laying hen’s performance.

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Data Availability

The datasets used and/or analyzed during the current research are available from the corresponding author on request.


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This study was approved and supported by the Isfahan University of Technology, Isfahan, Iran (grant no: 9306214).

Author information




Mohammad Mahdi Belkameh: conceptualization, data collection, writing, and software; Mohammad Sedghi: supervision, data curation, and methodology and editing; and Arash Azarfar: reviewing and editing.

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Correspondence to Mohammad Sedghi.

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The authors declare that they have no conflict of interest.

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This experiment was conducted according to the comprehensive guide of animal welfare adopted by the FASS [19]. All animal care and experimental procedures were approved by the animal policy and welfare committee of the Isfahan University of Technology.

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Belkameh, M.M., Sedghi, M. & Azarfar, A. The Effect of Different Levels of Dietary Magnesium on Eggshell Quality and Laying Hen’s Performance. Biol Trace Elem Res 199, 1566–1573 (2021).

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  • Laying hens
  • Magnesium
  • Eggshell quality
  • Performance
  • Egg internal quality