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Boron as Boric Acid Induces mRNA Expression of the Differentiation Factor Tuftelin in Pre-Osteoblastic MC3T3-E1 Cells

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Abstract

The effects of boron on the formation and maintenance of mineralized structures at the molecular level are still not clearly defined. Thus, a study was conducted using MC3T3-E1 cells to determine whether boron affected mRNA expressions of genes associated with bone/alveolar bone formation around the teethMC3T3-E1 (clone 4) cells were cultured in media treated with boric acid at concentrations of 0, 0.1, 10, 100, or 1000 ng/ml. Total RNAs of each group were isolated on day 3. Gene expression profiles were determined by using RT2 Profiler PCR micro-array that included 84 genes associated with osteogenic differentiation. Tuftelin1 mRNA expression was upregulated by all boron treatments. The upregulation was confirmed by quantitative RT-PCR using the tuftelin probe. While 100 ng/ml had no effect on the integrin-α2 (Itga2) transcript and 1 ng/ml boric acid induced Itga2 mRNA expression (2.1-fold), 0.1, 10, and 1000 ng/ml boric acid downregulated the integrin-α2 gene transcript 2.2-, 1.5-, and 2.1-fold respectively. While 0.1 ng/ml boric acid induced BMP6, increased BMP1r mRNA expression (1.5 fold) was observed in 1000 ng/ml boric acid treatment. The findings suggest that boron affects the regulation of the tuftelin1 gene in osteoblastic cells. Further studies are needed to establish that the beneficial actions of boron on alveolar bone and tooth formation and maintenance include an effect on the expression of the tuftelin1 gene.

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Funding

This study was funded from National Boron Research Institute, BOREN, Turkey (SSH). This work was performed at Selcuk University, Research Center of Dental Faculty, Konya, Turkey.

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Author notes

  1. Serife Buket Bozkurt

    Present address: Faculty of Dentistry, Research Lab of Dental Faculty, Hacettepe University, Ankara, Turkey

Authors and Affiliations

  1. Department of Periodontology, Faculty of Dentistry, Selcuk University, 42079, Konya, Turkey

    Sema S. Hakki

  2. Faculty of Dentistry, Research Center, Selcuk University, Konya, Turkey

    Sema S. Hakki & Serife Buket Bozkurt

  3. Faculty of Agriculture, Department of Soil Science and Plant Nutrition, Molecular Genetics & Biotechnology Laboratories, Selcuk University, Konya, Turkey

    Erdogan E. Hakki

  4. Research Nutritionist Consultant, 3000 Belmont Road, Grand Forks, ND, USA

    Forrest H. Nielsen

Authors
  1. Sema S. Hakki
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  2. Serife Buket Bozkurt
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  3. Erdogan E. Hakki
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  4. Forrest H. Nielsen
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Correspondence to Sema S. Hakki.

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Hakki, S.S., Bozkurt, S.B., Hakki, E.E. et al. Boron as Boric Acid Induces mRNA Expression of the Differentiation Factor Tuftelin in Pre-Osteoblastic MC3T3-E1 Cells. Biol Trace Elem Res 199, 1534–1543 (2021). https://doi.org/10.1007/s12011-020-02257-x

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  • DOI: https://doi.org/10.1007/s12011-020-02257-x

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