Impact of Coenzyme Q10 and Selenium on Seminal Fluid Parameters and Antioxidant Status in Men with Idiopathic Infertility

Abstract

Oxidative stress (OS) is a key contributing factor in 30–80% of male infertility cases. To date, several antioxidant treatments have been put forth to manage OS-induced male infertility. This study intended to elucidate the impact of coenzyme Q10 (CoQ10) and selenium on seminal fluid parameters and antioxidant status in infertile men with idiopathic oligoasthenoteratospermia (OAT). In this prospective study, 70 patients with idiopathic OAT were randomly allocated to receive CoQ10 (200 mg/day) or selenium (200 μg/day) for 3 months. Semen quality parameters (following WHO guidelines, 5th edition), total antioxidant capacity (TAC), catalase (CAT), and superoxide dismutase (SOD) activities were compared before and after the treatment. The results of the study showed an increase in sperm concentration with CoQ10 treatment (p < 0.01) as well as increased progressive sperm motility (p < 0.01 and p < 0.05) and total sperm motility (p < 0.01 and p < 0.05) with CoQ10 and selenium treatment respectively. There was also a significant improvement in TAC (p < 0.01 and p < 0.05) and SOD (p < 0.01 and p < 0.05) following treatment with CoQ10 and selenium respectively while CAT improved only with CoQ10 therapy (p < 0.05). Sperm concentration, motility, and morphology also correlated significantly with TAC, SOD, and CAT (r = 0.37–0.76). In conclusion, treatment with CoQ10 (200 mg) or selenium (200 μg) could improve sperm concentration, motility, and antioxidant status in infertile men with idiopathic OAT with CoQ10 providing the higher improvement.

This is a preview of subscription content, log in to check access.

References

  1. 1.

    Safarinejad MR (Jun. 2012) The effect of coenzyme Q10 supplementation on partner pregnancy rate in infertile men with idiopathic oligoasthenoteratozoospermia: an open-label prospective study. Int Urol Nephrol 44(3):689–700

    CAS  Article  Google Scholar 

  2. 2.

    Ko EY, Sabanegh ES, Agarwal A (Dec. 2014) Male infertility testing: reactive oxygen species and antioxidant capacity. Fertil Steril 102(6):1518–1527

    CAS  Article  Google Scholar 

  3. 3.

    Agarwal A, Leisegang K, Sengupta P (2020) Oxidative stress in pathology of male reproductive disorders. In: Preedy V (ed) Pathology: oxidative stress and dietary antioxidant, 1st edn. Elsevier

  4. 4.

    Arcaniolo D et al (2014) Is there a place for nutritional supplements in the treatment of idiopathic male infertility? Arch Ital Urol Androl 86(3):164

    CAS  Article  Google Scholar 

  5. 5.

    Dutta S, Majzoub A, Agarwal A (2019) Oxidative stress and sperm function: a systematic review on evaluation and management. Arab J Urol 17(2):87–97

  6. 6.

    Wagner H, Cheng JW, Ko EY (2018) Role of reactive oxygen species in male infertility: an updated review of literature. Arab J Urol 16(1):35–43

    Article  Google Scholar 

  7. 7.

    Bui AD, Sharma R, Henkel R, Agarwal A (2018) Reactive oxygen species impact on sperm DNA and its role in male infertility. Andrologia 50(8):e13012

  8. 8.

    Majzoub A, Agarwal A (2018) Systematic review of antioxidant types and doses in male infertility: Benefits on semen parameters , advanced sperm function , assisted reproduction and live-birth rate. Arab J Urol 16(1):113–124

    Article  Google Scholar 

  9. 9.

    Agarwal A, Arafa M, Chandrakumar R, Majzoub A, AlSaid S, Elbardisi H (2017) A multicenter study to evaluate oxidative stress by oxidation-reduction potential, a reliable and reproducible method. Andrology 5(5):939–945

    CAS  Article  Google Scholar 

  10. 10.

    Gvozdjáková A, Kucharská J, Dubravicky J, Mojto V, Singh RB (2015) Coenzyme Q10, $α$-tocopherol, and oxidative stress could be important metabolic biomarkers of male infertility. Dis Markers 2015:827941

    Article  Google Scholar 

  11. 11.

    Tremellen K (2008) Oxidative stress and male infertility--a clinical perspective. Hum Reprod Update 14(3):243–258

    CAS  Article  Google Scholar 

  12. 12.

    Alahmar A (2019) Role of oxidative stress in male infertility: an updated review. J Hum Reprod Sci 12(1):4–18

    CAS  Article  Google Scholar 

  13. 13.

    Agarwal A et al (2014) Characterizing semen parameters and their association with reactive oxygen species in infertile men. Reprod Biol Endocrinol 7(12):33

  14. 14.

    Aitken RJ (2017) DNA damage in human spermatozoa; important contributor to mutagenesis in the offspring. Transl Androl Urol 6(Suppl 4):S761–S764

    Article  Google Scholar 

  15. 15.

    Cardoso JP, Cocuzza M, Elterman D (2019) Optimizing male fertility: oxidative stress and the use of antioxidants. World J Urol 37(6):1029–1034

  16. 16.

    Smits RM, Mackenzie-Proctor R, Yazdani A, Stankiewicz M, Jordan V, Showell M (2019) Antioxidants for male subfertility. Cochrane Database Syst Rev 3(3):CD007411

  17. 17.

    Samplaski MK, Clemesha CG (2018) Discrepancies between the internet and academic literature regarding vitamin use for male infertility. Transl Androl Urol 7(Suppl 2):193–198

    Article  Google Scholar 

  18. 18.

    Alahmar AT (2018) The effects of oral antioxidants on the semen of men with idiopathic oligoasthenoteratozoospermia. Clin Exp Reprod Med 45(2):57–66

    Article  Google Scholar 

  19. 19.

    Beharry K, Cai C, Henry M, Chowdhury S, Valencia G, Aranda J (2017) Co-enzyme Q10 and n-3 polyunsaturated fatty Acid supplementation reverse intermittent hypoxia-induced growth restriction and improved antioxidant profiles in neonatal rats. Antioxidants 6(4):103

    Article  Google Scholar 

  20. 20.

    Nadjarzadeh A, Shidfar F, Amirjannati N, Vafa MR, Motevalian SA, Gohari MR, Nazeri Kakhki SA, Akhondi MM, Sadeghi MR (2014) Effect of coenzyme Q10 supplementation on antioxidant enzymes activity and oxidative stress of seminal plasma: a double-blind randomised clinical trial. Andrologia 46(2):177–183

    CAS  Article  Google Scholar 

  21. 21.

    Safarinejad MR (2009) Sexual function / infertility efficacy of coenzyme Q10 on semen parameters, Sperm. J Urol 182(1):237–248

    CAS  Article  Google Scholar 

  22. 22.

    Cakiroglu B, Eyyupoglu SE, Gozukucuk R, Uyanik BS (2014) Ubiquinol effect on sperm parameters in subfertile men who have astheno-teratozoospermia with normal sperm concentration. Nephrourol Mon 6(3):e16870

    Article  Google Scholar 

  23. 23.

    Imamovic Kumalic S, Pinter B (2014) Review of clinical trials on effects of oral antioxidants on basic semen and other parameters in idiopathic oligoasthenoteratozoospermia. Biomed Res Int 2014:1–11

    Article  Google Scholar 

  24. 24.

    Shalini S, Bansal MP (2005) Role of selenium in regulation of spermatogenesis : involvement of activator protein 1. Biofactors 22(3):151–162

  25. 25.

    Taghizadeh L, Eidi A, Mortazavi P, Rohani AH (2017) Effect of selenium on testicular damage induced by varicocele in adult male Wistar rats. J Trace Elem Med Biol 44:177–185

  26. 26.

    Moslemi MK, Tavanbakhsh S (2011) Selenium-vitamin E supplementation in infertile men: effects on semen parameters and pregnancy rate. Int J Gen Med 4:99–104

    CAS  Article  Google Scholar 

  27. 27.

    Bleau G, Lemarbre J, Faucher G, Roberts KD, Chapdelaine A (1984) Semen selenium and human fertility. Fertil Steril 42(6):890–894

    CAS  Article  Google Scholar 

  28. 28.

    Hawkes WC, Alkan Z, Wong K (2009) Selenium supplementation does not affect testicular selenium status or semen quality in North American men. J Androl 30(5):525–533

    CAS  Article  Google Scholar 

  29. 29.

    Mg S, Brown J, Yazdani A, Mt S, Rj H (2015) Antioxidants for male subfertility ( Review ). Cochrane Database Syst Rev (12):CD007411

  30. 30.

    Bisht S, Faiq M, Tolahunase M, Dada R (2017) Oxidative stress and male infertility. Nat Rev Urol 14(8):470–485

    CAS  Article  Google Scholar 

  31. 31.

    Balercia G, Buldreghini E, Vignini A, Tiano L, Paggi F, Amoroso S, Ricciardo-Lamonica G, Boscaro M, Lenzi A, Littarru GP (2009) Coenzyme Q10 treatment in infertile men with idiopathic asthenozoospermia: a placebo-controlled, double-blind randomized trial. Fertil Steril 91(5):1785–1792

    CAS  Article  Google Scholar 

  32. 32.

    World Health Organization (2010) WHO laboratory manual for the examination and processing of human semen, 5th edn. Cambridge University Press, Cambridge

  33. 33.

    World Health Organization (1999) WHO laboratory lanual for the examination of human semen and sperm-cervical mucus interaction, 4th edn. Cambridge University Press, Cambridge

  34. 34.

    Magnani L, Gaydou E, Hubaud JC (2000) Spectrophotometric measurement of antioxidant properties of flavones and flavonols against superoxide anion. Anal Chim Acta 411:209–216

  35. 35.

    Boscaro M, Lenzi A, Littarru G, M. D (2009) MALE FACTOR Coenzyme Q 10 treatment in infertile men with idiopathic asthenozoospermia : a placebo-controlled , double-blind randomized trial. Fertil Steril 91(5):1785–1792

    Article  Google Scholar 

  36. 36.

    Safarinejad MR, Safarinejad S, Shafiei N, Safarinejad S (Aug. 2012) Effects of the reduced form of coenzyme Q10 (ubiquinol) on semen parameters in men with idiopathic infertility: a double-blind, placebo controlled, randomized study. J Urol 188(2):526–531

    CAS  Article  Google Scholar 

  37. 37.

    Kobori Y et al (2014) Antioxidant cosupplementation therapy with vitamin C, vitamin E, and coenzyme Q10 in patients with oligoasthenozoospermia. Arch. Ital. di Urol. e Androl. 86(1):1

    Article  Google Scholar 

  38. 38.

    Kumalic I (2014) Review of Clinical Trials on Effects of Oral Antioxidants on Basic Semen and Other Parameters in Idiopathic Oligoasthenoteratozoospermia. Biomed Res Int 426951

  39. 39.

    Lafuente R, González-Comadrán M, Solà I, López G, Brassesco M, Carreras R, Checa MA (Sep. 2013) Coenzyme Q10 and male infertility: a meta-analysis. J Assist Reprod Genet 30(9):1147–1156

    Article  Google Scholar 

  40. 40.

    Ahmadi S, Bashiri R, Ghadiri-Anari A, Nadjarzadeh A (2016) Antioxidant supplements and semen parameters: an evidence based review. Int J Reprod Biomed (Yazd) 14(12):729–736

    CAS  Article  Google Scholar 

  41. 41.

    Gvozdjáková A, Kucharská J, Dubravicky J, Mojto V, Singh RB (2015) Coenzyme Q10, alpha-tocopherol, and oxidative stress could be important metabolic biomarkers of male infertility. Dis Markers 827941

  42. 42.

    Ghafarizadeh AA, Vaezi G (2017) Effect of in vitro selenium supplementation on sperm quality in asthenoteratozoospermic men. Andrologia 50(2):1–7

  43. 43.

    Pajovic B, Dimitrovski A, Radojevic N, Vukovic M (2016) A correlation between selenium and carnitine levels with hypo-osmotic swelling test for sperm membrane in low-grade varicocele patients. Eur Rev Med Pharmacol Sci 20(4):598–604

  44. 44.

    Morbat M, Hadi A, Hadri D (2018) Effect of selenium in treatment of male infertility. Exp Tech Urol Nephrol 1(5):1–4

  45. 45.

    Klein E (2004) Selenium: epidemiology and basic science. J Urol 171(2):50–53

    Google Scholar 

  46. 46.

    Hansen J, Deguchi Y (1996) Selenium and fertility in animals and man--a review. Acta Vet Scand 37(1):19–30

    PubMed  Google Scholar 

  47. 47.

    Eroglu M, Sahin S, Durukan B, Ozakpinar OB, Erdinc N, Turkgeldi L, Sofuoglu K, Karateke A (2014) Blood serum and seminal plasma selenium, total antioxidant capacity and coenzyme Q10 levels in relation to semen parameters in men with idiopathic infertility. Biol Trace Elem Res 159(1–3):46–51

    CAS  Article  Google Scholar 

  48. 48.

    Yousefniapasha Y, Jorsaraei G, Gholinezhadchariv M (2015) Nitric oxide levels and total antioxidant capacity in the seminal plasma of infertile smoking men. Cell J 17(1):129–136

    PubMed  PubMed Central  Google Scholar 

  49. 49.

    Micheli L, Cerretani D, Collodel G (2016) Evaluation of enzymatic and non-enzymatic antioxidants in seminal plasma of men with genitourinary infections, varicocele and idiopathic infertility. Andrology 4(3):456–464

    CAS  Article  Google Scholar 

  50. 50.

    Huang C, Cao X, Pang D, Li C (2018) Is male infertility associated with increased oxidative stress in seminal plasma? A-meta analysis. Oncotarget 9(36):24494–24513

    Article  Google Scholar 

  51. 51.

    Nadjarzadeh A et al (2011) Coenzyme Q10 improves seminal oxidative defense but does not affect on semen parameters in idiopathic oligoasthenoteratozoospermia: a randomized double-blind, placebo controlled trial. J Endocrinol Invest 34(8):e224–e228

  52. 52.

    Alahmar A (2017) Effect of vitamin C, vitamin E, zinc, selenium, and coenzyme Q10 in infertile men with idiopathic oligoasthenozoospermia. Int J Infertil Fetal Med 8(2):45–49

  53. 53.

    Agarwal A, Said TM (2003) Role of sperm chromatin abnormalities and DNA damage in male infertility. Hum Reprod Update 9(4):331–345

    CAS  Article  Google Scholar 

Download references

Acknowledgments

The authors would like to express their sincere gratitude for all patients who participated in this study for their valuable help to accomplish this work.

Author information

Affiliations

Authors

Corresponding author

Correspondence to Ahmed T. Alahmar.

Ethics declarations

Conflict of Interest

The authors declare that they have no conflict of interest.

Ethical Approval

All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (institutional and national) and with the Helsinki Declaration of 1975, as revised in 2008. The study protocol was approved by the local ethical committee at the University of Sumer, Iraq (EC/2018/8866), and all participants consented for participation in the study. The study was registered in clinicaltrials.gov (NCT03834831).

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Alahmar, A.T., Sengupta, P. Impact of Coenzyme Q10 and Selenium on Seminal Fluid Parameters and Antioxidant Status in Men with Idiopathic Infertility. Biol Trace Elem Res (2020). https://doi.org/10.1007/s12011-020-02251-3

Download citation

Keywords

  • CoQ10
  • Selenium
  • Semen
  • Antioxidants