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Effects of Dietary Iron Concentration on Manganese Utilization in Broilers Fed with Manganese-Lysine Chelate-Supplemented Diet

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Abstract

Dietary iron (Fe) influences manganese (Mn) utilization in chickens fed with inorganic Mn-supplemented diet. This study aimed to determine if dietary Fe levels affect Mn utilization in broilers fed with organic Mn-supplemented diet. Nine hundred 8-day-old broilers were randomly assigned to 1 of 6 treatments in a 3 (Fe level) × 2 (Mn source) factorial arrangement after feeding Mn- and Fe-unsupplemented diets for 7 days. The broilers were fed the basal diets (approximately 28 mg Mn/kg and 60 mg Fe/kg) supplemented with 0, 80, or 160 mg/kg Fe (L-Fe, M-Fe, or H-Fe), and 100 mg/kg Mn from Mn sulfate (MnSO4) or manganese-lysine chelate (MnLys) for 35 days. The H-Fe diet decreased (P < 0.05) body weight gain and feed intake as compared with L-Fe and M-Fe diets regardless of dietary Mn sources. Dietary Fe levels did not influence (P > 0.10) serum Mn concentration in MnLys-treated broilers, but serum Mn concentration decreased (P < 0.05) with dietary Fe increasing in MnSO4-treated broilers. The Mn concentration in the duodenum and tibia decreased (P < 0.05) with increasing dietary Fe levels regardless of dietary Mn sources, and MnLys increased (P < 0.04) these indices as compared with MnSO4. Dietary Fe levels did not significantly influence (P > 0.11) Mn concentration and activity and mRNA abundance of manganese-containing superoxide dismutase (MnSOD) in the heart of MnLys-treaded broilers, but the H-Fe diet decreased (P < 0.05) these indices in MnSO4-treated broilers as compared with M-Fe and L-Fe diets. The L-Fe diet increased (P < 0.001) duodenal divalent metal transporter 1 mRNA abundance when compared with the M-Fe and H-Fe diets on day 42, regardless of dietary Mn sources. The M-Fe and H-Fe diets decreased (P < 0.001) duodenal ferroportin 1 (FPN1) mRNA level when compared with the L-Fe diet in MnSO4-treated broilers, while dietary Fe levels did not significantly influence (P > 0.40) duodenal FPN1 mRNA abundance in MnLys-treated broilers. These results indicated dietary Fe levels decreased Mn utilization in MnSO4-treated broilers, but did not influence Mn utilization in MnLys-treated broilers evaluated by Mn concentrations in the serum and heart, and the activity and mRNA expression of heart MnSOD.

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Acknowledgments

The authors wish to thank Dr. Greg Fraley (Hope College, Holland, MI, USA) for the critical comments during the preparation of this manuscript.

Funding

This work was supported by the National Key Research and Development Program of China (2016YFD05005504), the National Natural Science Foundation of China (31001018), the Sichuan International Cooperation Project (2017HH0051), and the Chinese Chelota Group Research Project (2016LD0001).

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  1. Shiping Bai and Jialong Peng contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Animal Disease-resistant Nutrition, Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Huimin Road 211#, Wenjiang District, Chengdu, 611130, Sichuan, China

    Shiping Bai, Jialong Peng, Keying Zhang, Xuemei Ding, Jianping Wang, Qiufeng Zeng, Huanwei Peng, Jie Bai, Yue Xuan & Zuowei Su

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  1. Shiping Bai
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Correspondence to Shiping Bai.

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All animal care and treatment procedures used in the current study were approved by the Animal Care and Use Committee of Sichuan Agricultural University.

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Bai, S., Peng, J., Zhang, K. et al. Effects of Dietary Iron Concentration on Manganese Utilization in Broilers Fed with Manganese-Lysine Chelate-Supplemented Diet. Biol Trace Elem Res 198, 231–242 (2020). https://doi.org/10.1007/s12011-020-02035-9

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