Antimicrobial, Antioxidant and Larvicidal Activities of Spherical Silver Nanoparticles Synthesized by Endophytic Streptomyces spp.


In this study, metabolites involved in the free-biomass filtrates for three endophytic actinomycetes of Streptomyces capillispiralis Ca-1, Streptomyces zaomyceticus Oc-5, and Streptomyces pseudogriseolus Acv-11 were used as biocatalysts for green synthesis of silver nanoparticles (Ag-NPs). Characterization of biosynthesized Ag-NPs was accomplished using UV-Vis spectroscopy, X-ray diffraction patterns (XRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM-EDX), transmission electron microscopy (TEM), and particle size analyzer. The biosynthesized Ag-NPs showed maximum surface plasmon resonance (SPR) at 440 for strain Ca-1 and 450 for both strains of OC-5 and Acv-11. Nanoparticle spherical shape was recorded with size ranging from 23.77 to 63.14 nm, 11.32 to 36.72 nm, and 11.70 to 44.73 nm for Ca-1, Oc-5, and Acv-11, respectively. SEM-EDX analysis exhibited the weight percentages of 17.3, 22.3, and 48.7% for Ag-NPs synthesized by strains Ca-1, Oc-5 and Acv-11, respectively. The activities of biosynthesized Ag-NPs were concentration dependent and the obtained results confirmed the efficacy of Ag-NPs as antimicrobial agents against Gram-positive and Gram-negative bacteria as well unicellular and multicellular fungi. The MIC for Gram-positive bacteria, Gram-negative bacteria (E. coli), and eukaryotic microorganisms was 0.25 mM with clear zone ranging from 10.3 to 14.6 mm, while MIC for Pseudomonas aeruginosa was 1.0 mM for Ag-NPs synthesized by strain Ca-1 and 0.25 mM for those synthesized by strains Oc-5 and Acv-11. Moreover, Ag-NPs exhibited antimicrobial activity against four plant pathogenic fungi represented by Alternaria alternata, Fusarium oxysporum, Pythium ultimum, and Aspergillus niger at 2.0, 1.5, 1.0, and 0.5 mM of Ag-NPs with different degree. In vitro assessment of the antioxidant efficacy of biosynthesized Ag-NPs was achieved by scavenging assay of H2O2, reducing power of Fe3+, or total antioxidant assay. The results showed that antioxidant activities of Ag-NPs were concentration dependent with the highest activity at Ag-NP concentration of 2.0 mM. Furthermore, the biosynthesized NPs have prospective bioinsecticidal activity against Culex pipiens and Musca domestica. Green synthesis of NPs could be quite potential for the development of new bioactive compounds used in different biomedical applications.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7


  1. 1.

    Pasquoto-Stigliani T, Campos EV, Oliveira JL, Silva CM, Bilesky-José N, Guilger M, Troost J, Oliveira HC, Stolf-Moreira R, Fraceto LF (2017) Nanocapsules containing neem (Azadirachta Indica) oil: development, characterization, and toxicity evaluation. Sci Rep 7(1):5929

    PubMed  PubMed Central  Google Scholar 

  2. 2.

    Fouda A, Abdel-Maksoud G, Abdel-Rahman MA, Salem SS, Hassan SE-D, El-Sadany MA-H (2019) Eco-friendly approach utilizing green synthesized nanoparticles for paper conservation against microbes involved in biodeterioration of archaeological manuscript. Int Biodeterior Biodegradation 142:160–169

    CAS  Google Scholar 

  3. 3.

    Fouda A, Abdel-Maksoud G, Abdel-Rahman MA, Eid AM, Barghoth MG, El-Sadany MA-H (2019) Monitoring the effect of biosynthesized nanoparticles against biodeterioration of cellulose-based materials by Aspergillus niger. Cellulose, 26(11):6583–6597

  4. 4.

    Naghizadeh A, Ghafouri M (2017) Synthesis and performance evaluation of chitosan prepared from Persian gulf shrimp shell in removal of reactive blue 29 dye from aqueous solution (isotherm, thermodynamic and kinetic study). Iranian Journal of Chemistry and Chemical Engineering 36(3):25–36

    CAS  Google Scholar 

  5. 5.

    Naghizadeh A, Nabizadeh R (2016) Removal of reactive blue 29 dye by adsorption on modified chitosan in the presence of hydrogen peroxide. Environ Prot Eng 42(1):149–168

  6. 6.

    Naghizadeh A, Kamranifar M, Yari AR, Mohammadi MJ (2017) Equilibrium and kinetics study of reactive dyes removal from aqueous solutions by bentonite nanoparticles. Desalin Water Treat 97:329–337

    CAS  Google Scholar 

  7. 7.

    Kamranifar M, Masoudi F, Naghizadeh A, Asri M (2019) Fabrication and characterization of magnetic cobalt ferrite nanoparticles for efficient removal of humic acid from aqueous solutions. Desalin Water Treat 144:233–242

    CAS  Google Scholar 

  8. 8.

    Gu H, Ho P, Tong E, Wang L, Xu B (2003) Presenting vancomycinonnanoparticles to enhance antimicrobial activities. Nano Lett 3(9):1261–1263

    CAS  Google Scholar 

  9. 9.

    Fouda A, Saad E, Salem SS, Shaheen TI (2018) In-vitro cytotoxicity, antibacterial, and UV protection properties of the biosynthesized zinc oxide nanoparticles for medical textile applications. Microb Pathog 125:252–261

    CAS  Google Scholar 

  10. 10.

    Mohmed AA, Fouda A, Abdel-Rahman MA, Hassan SE–D, El-Gamal MS, Salem SS, Shaheen TI (2019) Fungal strain impacts the shape, bioactivity and multifunctional properties of green synthesized zinc oxide nanoparticle. Biocatal Agric Biotechnol. 19. 101103

  11. 11.

    Hassan SED, Salem SS, Fouda A, Awad MA, El-Gamal MS, Abdo AM (2018) New approach for antimicrobial activity and bio-control of various pathogens by biosynthesized copper nanoparticles using endophytic actinomycetes. Journal of Radiation Research and Applied Sciences 11(3):262–270

    CAS  Google Scholar 

  12. 12.

    Hassan SE-D, Fouda A, Radwan AA, Salem SS, Barghoth MG, Awad MA, Abdo AM, El-Gamal MS (2019) Endophytic actinomycetes Streptomyces spp mediated biosynthesis of copper oxide nanoparticles as a promising tool for biotechnological applications. J Biol Inorg Chem. 24(3):377–393. 

  13. 13.

    Gupta RK, Kumar V, Gundampati RK, Malviya M, Hasan SH, Jagannadham MV (2017) Biosynthesis of silver nanoparticles from the novel strain of Streptomyces sp. BHUMBU-80 with highly efficient electroanalytical detection of hydrogen peroxide and antibacterial activity. J Environ Chem Eng 5(6):5624–5635

    CAS  Google Scholar 

  14. 14.

    Rasheed T, Bilal M, Iqbal HMN, Li CL (2017) Green biosynthesis of silver nanoparticles using leaves extract of Artemisia vulgaris and their potential biomedical applications. Colloid Surf B 2017:408–415

    Google Scholar 

  15. 15.

    Mohmed AA, Fouda A, Elgamal MS, Hassan S, Shaheen TI, Salem SS (2017) Enhancing of cotton fabric antibacterial properties by silver nanoparticles synthesized by new Egyptian strain Fusarium keratoplasticum A1-3. Egyptian journal of chemistry 60 (conference issue (the 8th international conference of the textile research division (ICTRD 2017), National Research Centre, Cairo 12622, Egypt.)) 63-71

  16. 16.

    Durán N, Durán M, de Jesus MB, Seabra AB, Fávaro WJ, Nakazato G (2016) Silver nanoparticles: a new view on mechanistic aspects on antimicrobial activity. Nanomed Nanotech Biol Med 12(3):789–799

    Google Scholar 

  17. 17.

    Zhang Q, Li Y, Xu D, Gu Z (2001) Preparation of silver nanowire arrays in anodic aluminum oxide templates. J Mater Sci Lett 20(10):925–927

    CAS  Google Scholar 

  18. 18.

    Yin AJ, Li J, Jian W, Bennett J, Xu JH (2001) Fabrication of highly ordered metallic nanowire arrays by electrodeposition. Appl Phys Lett 79(7):1039–1041

    CAS  Google Scholar 

  19. 19.

    Veerasamy R, Xin TZ, Gunasagaran S, Xiang TFW, Yang EFC, Jeyakumar N, Dhanaraj SA (2011) Biosynthesis of silver nanoparticles using mangosteen leaf extract and evaluation of their antimicrobial activities. Journal of Saudi Chemical Society 15(2):113–120

    CAS  Google Scholar 

  20. 20.

    Saha N, Gupta SD (2017) Low-dose toxicity of biogenic silver nanoparticles fabricated by Swertiachirataon roots tips and flower buds of Allium cepa. J Hazard Mat 330:18–28

    CAS  Google Scholar 

  21. 21.

    Ahmed S, Ahmad M, Swami BL, Ikram S (2016) A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: a green expertise. J Adv Res 7:17–28

    CAS  PubMed  Google Scholar 

  22. 22.

    Duran N, Priscyla D, Marcato PD, Alves O, De Souza G, Esposito E (2005) Mechanistic aspects of biosynthesis of silver nanoparticles by several Fusarium oxysporum strains. J Nanobiotechnol 3:1–7

    Google Scholar 

  23. 23.

    Tsibakhashvili NY, Kirkesali EI, Pataraya DT, Gurielidze MA, Kalabegishvili TL, Gvarjaladze DN, Tsertsvadze GI, Frontasyeva MV, Zinicovscaia II, Wakstein MS, Khakhanov SN, Shvindina NV, Shklover VY (2011) Microbial synthesis of silver nanoparticles by Streptomyces glaucus and Spirulina platensis. Advan Sci Lett 4:1–10

    Google Scholar 

  24. 24.

    Fouda A., Hassan S.E., Eid A.M., El-Din Ewais E. (2019) The Interaction Between Plants and Bacterial Endophytes Under Salinity Stress. In: Jha S. (eds) Endophytes and Secondary Metabolites. Reference Series in Phytochemistry. Springer, Cham

  25. 25.

    Eid AM, Salim SS, Hassan SE-D, Ismail MA, Fouda A (2019) Role of endophytes in plant health and abiotic stress management. In: Kumar V, Prasad R, Kumar M, Choudhary DK (eds) Microbiome in plant health and disease: challenges and opportunities. Springer Singapore, Singapore, pp 119–144.

    Google Scholar 

  26. 26.

    Hulkoti NI, Taranath TC (2014) Biosynthesis of nanoparticles using microbes- a review. Colloids Surf B: Biointerfaces 121:474–483.

    CAS  Article  PubMed  Google Scholar 

  27. 27.

    Sadhasivam S, Shanmugam P, Yun K (2010) Biosynthesis of silver nanoparticles by Streptomyces hygroscopicus and antimicrobial activity against medically important pathogenic microorganisms. Colloids Surf B: Biointerfaces 81:358–362

    CAS  PubMed  Google Scholar 

  28. 28.

    Sivalingam P, Antony JJ, Siva D, Achiraman S, Anbarasu K (2012) Mangrove Streptomyces sp. BDUKAS10 as nanofactory for fabrication of bactericidal silver nanoparticles. Colloids Surf B: Biointerfaces 98:12–17

    CAS  PubMed  Google Scholar 

  29. 29.

    Manivasagan P, Venkatesan J, Senthilkumar K, Sivakumar K, Kim SK (2013) Biosynthesis, antimicrobial and cytotoxic effect of silver nanoparticles using a novel Nocardiopsis sp. MBRC–1. BioMed Res Int 2013. Article ID 287638. 9 pages

  30. 30.

    Karthik L, Kumar G, Vishnu Kirthi A, Rahuman AA, Bhaskara Rao KV (2014) Streptomyces sp. LK3 mediated synthesis of silver nanoparticles and its biomedical application. Bioprocess Biosyst Eng 37:261–267

    CAS  PubMed  Google Scholar 

  31. 31.

    Saravanakumar P, Balachandran C, Duraipandiyan V, Ramasamy D, Ignacimuthu S, Al-Dhabi NA (2014) Extracellular biosynthesis of silver nanoparticle using Streptomyces sp. 09 PBT 005 and its antibacterial and cytotoxic properties. Appl Nanosci 5:169–180

    Google Scholar 

  32. 32.

    Subbaiya R, Saravanan M, Priya AR, Shankar KR, Selvam M, Ovais M, Balajee R, Barabadi H (2017) Biomimetic synthesis of silver nanoparticles from Streptomyces atrovirens and their potential anticancer activity against human breast cancer cells. IET Nanobiotechnol 11(8):965–972

    PubMed  Google Scholar 

  33. 33.

    Valgas C, Souza SMD, Smânia EF, Smânia A Jr (2007) Screeningmethods to determine antibacterial activity of natural products. Braz J Microbiol 38:369–380

    Google Scholar 

  34. 34.

    Mahdizadeh V, Safaie N, Khelghatibana F (2015) Evaluation of antifungal activity of silver nanoparticles against some phytopathogenic fungi and Trichoderma harzianum. J Crop Prot 4(3):291–300

    Google Scholar 

  35. 35.

    Gulcin I, Kufervioglu OI, Oktay M, Buyukokuroglu ME (2004) Antioxidant, antimicrobial, antiulcer and analgesic activities of nettle (urtica dioica L.). Journal of Ethnopharmacology 90(2–3):205–215

  36. 36.

    Oyaizu M (1986) Studies on products of browning reactions: antioxidative activites of products of browning reaction prepared from glucosamine. Jpn J Nutr 44

  37. 37.

    Prieto P, Pineda M, Anguilar M (1999) Spectrophotometric quantitation of antioxidant capacity through the formatioon of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem 269:337–341

    CAS  PubMed  Google Scholar 

  38. 38.

    Kamaraj C, Bagavan A, Rahuman AA, Zahir AA, Elango G, Pandiyan G (2009) Larvicidal potential of medicinal plant extracts against Anopheles subpictus grassi and Culex tritaeniorhynchus Giles (Diptera: Culicidae). Parasitol Res 104(5):1163–1171

    CAS  PubMed  Google Scholar 

  39. 39.

    Busvine JR (1962) A laboratory technique for measuring the susceptibility of houseflies and blow flies to insecticides. Lab Prot 11:464–468

    Google Scholar 

  40. 40.

    Ahmed MK, Abdulah S, Mohammed N (2018) Evaluation of some insecticides against Culex pipiens, the dominant mosquito species in Abha city. Int J Hortic Agric Food Sci 2(2):4–17

    Google Scholar 

  41. 41.

    Abbott WS (1925) A method of computing the effectiveness of an insecticide. J Econ Entomol 18:265–267

    CAS  Google Scholar 

  42. 42.

    Abdelghany TM, Al–Rajhi AM, Al Abboud MA, Alawlaqi MM, Magdah AG, Helmy EA, Mabrouk AS (2017) Recent advances in green synthesis of silver nanoparticles and their applications: about future directions. A Review. BioNanoScience. 8(1):5–16.

  43. 43.

    EL-Moslamy SH (2018) Bioprocessing strategies for cost-effective large-scale biogenic synthesis of nano-MgO from endophytic Streptomyces coelicolor strain E72 as an anti-multidrug-resistant pathogens agent. Sci Rep 28;8(1):3820

  44. 44.

    Ibrahim HM (2015) Green synthesis and characterization of silver nanoparticles using banana peel extract and their antimicrobial activity against representative microorganisms. J Radiat Res Appl Sci 8(3):265–275

    Google Scholar 

  45. 45.

    Behravan M, Panahi AH, Naghizadeh A, Ziaee M, Mahdavi R, Mirzapour A (2019) Facile green synthesis of silver nanoparticles using Berberis vulgaris leaf and root aqueous extract and its antibacterial activity. Int J Biol Macromol 124:148–154

    CAS  PubMed  Google Scholar 

  46. 46.

    Dong Z-Y, Narsing Rao MP, Xiao M, Wang H-F, Hozzein W, Chen W, Li W-J (2017) Antibacterial Activity of Silver Nanoparticles against Staphylococcus warneri Synthesized Using Endophytic Bacteria by Photo-irradiation. Front Microbiol 8. doi:

  47. 47.

    Maria BS, Devadiga A, Kodialbail VS, Saidutta M (2015) Synthesis of silver nanoparticles using medicinal Zizyphus xylopyrus bark extract. Appl Nanosci 5(6):755–762

    Google Scholar 

  48. 48.

    Vijayabharathi R, Sathya A, Gopalakrishnan S (2018) Extracellular biosynthesis of silver nanoparticles using Streptomyces griseoplanus SAI-25 and its antifungal activity against Macrophomina phaseolina, the charcoal rot pathogen of sorghum. Biocatalysis and Agricultural Biotechnology 14:166–171

    Google Scholar 

  49. 49.

    Kim TH, Kim M, Park HS, Shin US, Gong MS, Kim HW (2012) Size-dependent cellular toxicity of silver nanoparticles. J Biomed Mater Res A 100(4):1033–1043

    PubMed  Google Scholar 

  50. 50.

    Dehghani MH, Naghizadeh A, Rashidi A, Derakhshani E (2013) Adsorption of reactive blue 29 dye from aqueous solution by multiwall carbon nanotubes. Desalin Water Treat 51(40–42):7655–7662

    CAS  Google Scholar 

  51. 51.

    Derakhshani E, Naghizadeh A (2018) Optimization of humic acid removal by adsorption onto bentonite and montmorillonite nanoparticles. J Mol Liq 259:76–81

    CAS  Google Scholar 

  52. 52.

    Babu MMG, Sridhar J, Gunasekaran P (2011) Global transcriptome analysis of Bacillus cereus ATCC 14579 in response to silver nitrate stress. Journal of Nanobiotechnology 9(1):49

    PubMed  Google Scholar 

  53. 53.

    Magudapathy P, Gangopadhyay P, Panigrahi B, Nair K, Dhara S (2001) Electrical transport studies of ag nanoclusters embedded in glass matrix. Phys B Condens Matter 299(1–2):142–146

    CAS  Google Scholar 

  54. 54.

    Tomaszewska E, Soliwoda K, Kadziola K, Tkacz-Szczesna B, Celichowski G, Cichomski M, Szmaja W, Grobelny J (2013) Detection limits of DLS and UV-vis spectroscopy in characterization of polydisperse nanoparticles colloids. J Nanomater 2013:60

    Google Scholar 

  55. 55.

    Singh T, Jyoti K, Patnaik A, Singh A, Chauhan R, Chandel S (2017) Biosynthesis, characterization and antibacterial activity of silver nanoparticles using an endophytic fungal supernatant of Raphanus sativus. Journal of Genetic Engineering and Biotechnology 15(1):31–39

    PubMed  Google Scholar 

  56. 56.

    Shaheen TI, Fouda A (2018) Green approach for one-pot synthesis of silver nanorod using cellulose nanocrystal and their cytotoxicity and antibacterial assessment. Int J Biol Macromol 106:784–792

    CAS  PubMed  Google Scholar 

  57. 57.

    Ge L, Li Q, Wang M, Ouyang J, Li X, Xing MM (2014) Nanosilver particles in medical applications: synthesis, performance, and toxicity. Int J Nanomedicine 9:2399

    PubMed  PubMed Central  Google Scholar 

  58. 58.

    Naghizadeh A (2016) Regeneration of carbon nanotubes exhausted with humic acid using electro-Fenton technology. Arab J Sci Eng 41(1):155–161

    CAS  Google Scholar 

  59. 59.

    Chatterjee T, Chatterjee BK, Majumdar D, Chakrabarti P (2015) Antibacterial effect of silver nanoparticles and the modeling of bacterial growth kinetics using a modified Gompertz model. Biochimica et Biophysica Acta (BBA)-General Subjects 1850(2):299–306

    CAS  Google Scholar 

  60. 60.

    Abbaszadegan A, Ghahramani Y, Gholami A, Hemmateenejad B, Dorostkar S, Nabavizadeh M, Sharghi H (2015) The effect of charge at the surface of silver nanoparticles on antimicrobial activity against gram-positive and gram-negative bacteria: a preliminary study. J Nanomater 16(1):53

    Google Scholar 

  61. 61.

    Slavin YN, Asnis J, Häfeli UO, Bach H (2017) Metal nanoparticles: understanding the mechanisms behind antibacterial activity. Journal of Nanobiotechnology 15(1):65

    PubMed  PubMed Central  Google Scholar 

  62. 62.

    Ahmad A, Wei Y, Syed F, Tahir K, Rehman AU, Khan A, Ullah S, Yuan Q (2017) The effects of bacteria-nanoparticles interface on the antibacterial activity of green synthesized silver nanoparticles. Microb Pathog 102:133–142

    CAS  PubMed  Google Scholar 

  63. 63.

    Sharaf OM, Al-Gamal MS, Ibrahim GA, Dabiza NM, Salem SS, El-ssayad MF, Youssef AM (2019) Evaluation and characterization of some protective culture metabolites in free and nano-chitosan-loaded forms against common contaminants of Egyptian cheese. Carbohydr Polym 223:115094.

    CAS  Article  PubMed  Google Scholar 

  64. 64.

    Ravichandran A, Subramanian P, Manoharan V, Muthu T, Periyannan R, Thangapandi M, Ponnuchamy K, Pandi B, Marimuthu PN (2018) Phyto-mediated synthesis of silver nanoparticles using fucoidan isolated from Spatoglossum asperum and assessment of antibacterial activities. J Photochem Photobiol B Biol 185:117–125

    CAS  Google Scholar 

  65. 65.

    Abalkhil TA, Alharbi SA, Salmen SH, Wainwright M (2017) Bactericidal activity of biosynthesized silver nanoparticles against human pathogenic bacteria. Biotechnology & Biotechnological Equipment 31(2):411–417

    CAS  Google Scholar 

  66. 66.

    Jin J-C, Wu X-J, Xu J, Wang B-B, Jiang F-L, Liu Y (2017) Ultrasmall silver nanoclusters: highly efficient antibacterial activity and their mechanisms. Biomaterials Science 5(2):247–257

    CAS  PubMed  Google Scholar 

  67. 67.

    Feng QL, Wu J, Chen G, Cui F, Kim T, Kim J (2000) A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. J Biomed Mater Res 52(4):662–668

    CAS  Google Scholar 

  68. 68.

    Manke A, Wang L, Rojanasakul Y (2013) Mechanisms of nanoparticle-induced oxidative stress and toxicity. Biomed Res Int 2013:1–15

    Google Scholar 

  69. 69.

    Roy A, Bulut O, Some S, Mandal AK, Yilmaz MD (2019) Green synthesis of silver nanoparticles: biomolecule-nanoparticle organizations targeting antimicrobial activity. RSC Adv 9:2673–2702

    CAS  Google Scholar 

  70. 70.

    Thenmozhi M, Kannabiran K, Kumar R, Khanna VG (2013) Antifungal activity of Streptomyces sp. VITSTK7 and its synthesized Ag2O/ag nanoparticles against medically important Aspergillus pathogens. Journal de Mycologie Medicale 23(2):97–103

    CAS  PubMed  Google Scholar 

  71. 71.

    Danilczuk M, Lund A, Sadlo J, Yamada H, Michalik J (2006) Conduction electron spin resonance of small silver particles. Spectrochim Acta A Mol Biomol Spectrosc 63(1):189–191

    CAS  PubMed  Google Scholar 

  72. 72.

    Schieber M, Chandel NS (2014) ROS function in redox signaling and oxidative stress. Curr Biol 24(10):R453–R462

    CAS  PubMed  PubMed Central  Google Scholar 

  73. 73.

    Wu D, Cederbaum AI (2003) Alcohol, oxidative stress, and free radical damage. Alcohol Res Health 27:277–284

    PubMed  PubMed Central  Google Scholar 

  74. 74.

    Miller MJ, Sadowska-Krowicka H, Chotinaruemol S, Kakkis JL, Clark DA (1993) Amelioration of chronic ileitis by nitricoxide synthase inhibition. J Pharmacol Exp Ther 264(1):11-16

  75. 75.

    Moein M, Moein S, Ahmadizadeh S (2008) Radical scavenging and reducing power of Salvia mirzayanii subfractions. Molecules 13(11):2804–2813

    CAS  PubMed  PubMed Central  Google Scholar 

  76. 76.

    Sudha A, Jeyakanthan J, Srinivasan P (2017) Green synthesis of silver nanoparticles using Lippia nodiflora aerial extract and evaluation of their antioxidant, antibacterial and cytotoxic effects. Resource-Efficient Technologies 3(4):506–515

    Google Scholar 

  77. 77.

    Cyril N, George JB, Joseph L, Raghavamenon A, Sylas V (2019) Assessment of antioxidant, antibacterial and anti-proliferative (lung cancer cell line A549) activities of green synthesized silver nanoparticles from Derris trifoliata. Toxicology Research 8(2):297–308

    CAS  PubMed  PubMed Central  Google Scholar 

  78. 78.

    Bhakya S, Muthukrishnan S, Sukumaran M, Muthukumar M (2016) Biogenic synthesis of silver nanoparticles and their antioxidant and antibacterial activity. Appl Nanosci 6(5):755–766

    CAS  Google Scholar 

  79. 79.

    Torres S, Campos V, León C, Rodríguez-Llamazares S, Rojas S, Gonzalez M, Smith C, Mondaca M (2012) Biosynthesis of selenium nanoparticles by Pantoea agglomerans and their antioxidant activity. J Nanopart Res 14(11):1236

    Google Scholar 

  80. 80.

    Cai W, Hu T, Bakry AM, Zheng Z, Xiao Y, Huang Q (2018) Effect of ultrasound on size, morphology, stability and antioxidant activity of selenium nanoparticles dispersed by a hyperbranched polysaccharide from Lignosus rhinocerotis. Ultrason Sonochem 42:823–831

    CAS  PubMed  Google Scholar 

  81. 81.

    Sharma P, Bhatt D, Zaidi M, Saradhi PP, Khanna P, Arora S (2012) Silver nanoparticle-mediated enhancement in growth and antioxidant status of brassica juncea. Appl Biochem Biotechnol 167(8):2225–2233

    CAS  PubMed  PubMed Central  Google Scholar 

  82. 82.

    Patra JK, Baek K-H (2016) Biosynthesis of silver nanoparticles using aqueous extract of silky hairs of corn and investigation of its antibacterial and anticandidal synergistic activity and antioxidant potential. IET Nanobiotechnol 10(5):326–333

    PubMed  Google Scholar 

  83. 83.

    Suresh U, Murugan K, Panneerselvam C, Rajaganesh R, Roni M, Al-Aoh HAN, Trivedi S, Rehman H, Kumar S, Higuchi A (2018) Suaeda maritima-based herbal coils and green nanoparticles as potential biopesticides against the dengue vector Aedes aegypti and the tobacco cutworm Spodoptera litura. Physiol Mol Plant Pathol 101:225–235

    CAS  Google Scholar 

  84. 84.

    Benelli G, Pavela R, Maggi F, Petrelli R, Nicoletti M (2017) Commentary: making green pesticides greener? The potential of plant products for nanosynthesis and pest control. J Clust Sci 28(1):3–10

    CAS  Google Scholar 

Download references


Authors are greatly thankful to Dr. Salem S. Salem for their great support and contributions through this work and Dr. Mohamed A. Awad for helping in larvicidal bioassay.


This work was not supported by any funding.

Author information



Corresponding author

Correspondence to Amr Fouda.

Ethics declarations

Conflict of Interest

The authors declare that they have no conflicts of interest.

Data Availability

The data used to support the findings of this study are available from the corresponding author upon request.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Fouda, A., Hassan, S.ED., Abdo, A.M. et al. Antimicrobial, Antioxidant and Larvicidal Activities of Spherical Silver Nanoparticles Synthesized by Endophytic Streptomyces spp.. Biol Trace Elem Res 195, 707–724 (2020).

Download citation


  • Endophytic Streptomyces spp.
  • Silver nanoparticles
  • Antimicrobial activity
  • Phytopathogenic fungi
  • Antioxidant activity
  • Bioinsecticidal