Gentamicin-Assisted Mycogenic Selenium Nanoparticles Synthesized Under Gamma Irradiation for Robust Reluctance of Resistant Urinary Tract Infection-Causing Pathogens


The purpose of this research is to compare and enhance the antimicrobial and antibiofilm potentials of the biogenic selenium nanoparticles (Se NPs) produced by cost-effective and eco-friendly green methods. The synthesis of Se NPs is described in this manuscript by two different methods: a biogenic process using Penicillium chrysogenum filtrate and by utilizing gentamicin drug (CN) following the application of gamma irradiation. Se NPs were characterized by UV-Vis., HRTM, FTIR, XRD, DLS, SEM, and EDX mapping technique. Antimicrobial and antibiofilm activities of the synthesized Se NPs were investigated against multidrug-resistant (MDR) bacteria and yeast causing severe diseases such as urinary tract infection (UTI). The biogenic Se NPs exhibited an absorption peak at 435.0 nm while Se NPs-CN showed an absorption peak at 350.0 nm which is related to the surface plasmon resonance (SPR). Data obtained from HRTEM, SEM/mapping, and XRD analysis confirmed the mono-dispersion and crystalline nature of the prepared samples with an average diameter of 33.84 nm and 22.37 nm for the mycogenic Se NPs and Se NPs-CN, respectively. The synthesized Se NPs-CN possesses an encouraging antimicrobial potential with respect to the biogenic Se NPs against all examined UTI-causing microbes. Remarkably, Se NPs-CN showed antimicrobial potential toward Candida albicans with a zone of Inhibition (ZOI) recorded at 26.0 mm, 23.0 mm ZOI for Escherichia coli and 20.0 mm ZOI against Staphylococcus aureus. In addition, the incorporated Se NPs-CN displayed an enhanced percentage of biofilm inhibition of 88.67%, 87.93%, and 85.20% against S. aureus, P. aeruginosa, and E. coli, respectively. Accordingly, the novelty of the present research involves the green synthesis of mono-dispersed Se NPs and combining the synergistic potential of CN with Se NPs for potential biomedical, pharmaceutical, and therapeutic applications especially in the treatment of UTI.

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  1. 1.

    Abd Elkodous M, el-Sayyad GS, Nasser HA, Elshamy AA, Morsi M, Abdelrahman IY, Kodous AS, Mosallam FM, Gobara M, el-Batal AI (2019) Engineered nanomaterials as potential candidates for HIV treatment: between opportunities and challenges. J Clust Sci 30(3):531–540

    CAS  Google Scholar 

  2. 2.

    Abd Elkodous M, el-Sayyad GS, Abdelrahman IY, el-Bastawisy HS, Mohamed AE, Mosallam FM, Nasser HA, Gobara M, Baraka A, Elsayed MA, el-Batal AI (2019) Therapeutic and diagnostic potential of nanomaterials for enhanced biomedical applications. Colloids Surf B: Biointerfaces 180:411–428

    CAS  PubMed  Google Scholar 

  3. 3.

    Thirugnanasambandan T, Pal K, A. S, Elkodous MA, Prasath H, Kulasekarapandian K, Ayeshamariam A, Jeevanandam J (2018) Aggrandize efficiency of ultra-thin silicon solar cell via topical clustering of silver nanoparticles. Nano-Struct Nano-Objects 16:224–233

    CAS  Google Scholar 

  4. 4.

    Sarkar J, Dey P, Saha S, Acharya K (2011) Mycosynthesis of selenium nanoparticles. Micro Nano Lett 6(8):599–602

    CAS  Google Scholar 

  5. 5.

    El-Ghazaly MA et al (2016) Anti-inflammatory effect of selenium nanoparticles on the inflammation induced in irradiated rats. Can J Physiol Pharmacol 95(2):101–110

    PubMed  Google Scholar 

  6. 6.

    Jeevanandam J, Pal K, Danquah MK (2018) Virus-like nanoparticles as a novel delivery tool in gene therapy. Biochimie

  7. 7.

    El-Batal AI et al (2019. In press) Penicillium chrysogenum-mediated mycogenic synthesis of copper oxide nanoparticles using gamma rays for in vitro antimicrobial activity against some plant pathogens. J Clust Sci.

  8. 8.

    El-Batal AI et al (2019) Potential Nematicidal properties of silver boron nanoparticles: synthesis, characterization, in vitro and in vivo root-knot nematode (Meloidogyne incognita) treatments. J Clust Sci 30(3):687–705

    CAS  Google Scholar 

  9. 9.

    Elkodous MA, et al. (2018) C-dots dispersed macro-mesoporous TiO2 phtocatalyst for effective waste water treatment. Charact Appl Nanomat 1(2):1–12

  10. 10.

    Iranifam M, Fathinia M, Sadeghi Rad T, Hanifehpour Y, Khataee AR, Joo SW (2013) A novel selenium nanoparticles-enhanced chemiluminescence system for determination of dinitrobutylphenol. Talanta 107:263–269

    CAS  PubMed  Google Scholar 

  11. 11.

    Van Overschelde O, Guisbiers G, Snyders R (2013) Green synthesis of selenium nanoparticles by excimer pulsed laser ablation in water. APL Mater 1(4):042114

    Google Scholar 

  12. 12.

    Quintana M, Haro-Poniatowski E, Morales J, Batina N (2002) Synthesis of selenium nanoparticles by pulsed laser ablation. Appl Surf Sci 195(1-4):175–186

    CAS  Google Scholar 

  13. 13.

    Sagadevan S et al (2017) Hydrothermal synthesis of zinc stannate nanoparticles spectroscopic investigation. J Mater Sci Mater Electron 28(15):11268–11274

    CAS  Google Scholar 

  14. 14.

    Sajjadifar S et al (2019) Characterization of catalyst: comparison of brønsted and lewis acidic power in boron sulfonic acid as a heterogeneous catalyst in green synthesis of quinoxaline derivatives. Chem Methodol 3(2. pp. 145-275):226–236

    CAS  Google Scholar 

  15. 15.

    El-Sayyad GS, Mosallam FM, El-Batal AI (2018) One-pot green synthesis of magnesium oxide nanoparticles using Penicillium chrysogenum melanin pigment and gamma rays with antimicrobial activity against multidrug-resistant microbes. Adv Powder Technol 29(11):2616–2625

    CAS  Google Scholar 

  16. 16.

    El-Batal AI et al (2017) Response surface methodology optimization of melanin production by Streptomyces cyaneus and synthesis of copper oxide nanoparticles using gamma radiation. J Clust Sci 28(3):1083–1112

    CAS  Google Scholar 

  17. 17.

    El-Batal AI et al (2017) Melanin-gamma rays assistants for bismuth oxide nanoparticles synthesis at room temperature for enhancing antimicrobial, and photocatalytic activity. J Photochem Photobiol B Biol 173:120–139

    CAS  Google Scholar 

  18. 18.

    El-Batal A et al (2013) Synthesis of selenium nanoparticles by Bacillus laterosporus using gamma radiation. World Appl Sci J 22(1):1–16

    CAS  Google Scholar 

  19. 19.

    El-Batal AI et al (2017) Antimicrobial, antioxidant and anticancer activities of zinc nanoparticles prepared by natural polysaccharides and gamma radiation. Int J Biol Macromol 107:2298–2311

    PubMed  Google Scholar 

  20. 20.

    Dwivedi C, Shah CP, Singh K, Kumar M, Bajaj PN (2011) An organic acid-induced synthesis and characterization of selenium nanoparticles. J Nanotechnol 2011:16

    Google Scholar 

  21. 21.

    Lin ZH, Lin FC, Wang CC (2004) Observation in the growth of selenium nanoparticles. J Chin Chem Soc 51(2):239–242

    CAS  Google Scholar 

  22. 22.

    Zhang Y, Wang J, Zhang L (2010) Creation of highly stable selenium nanoparticles capped with hyperbranched polysaccharide in water. Langmuir 26(22):17617–17623

    CAS  PubMed  Google Scholar 

  23. 23.

    Pal K, Yang X, Mohan MLNM, Schirhagl R, Wang G (2016) Switchable, self-assembled CdS nanomaterials embedded in liquid crystal cell for high performance static memory device. Mater Lett 169:37–41

    CAS  Google Scholar 

  24. 24.

    Pal K, Mohan MLNM, Zhan B, Wang G (2015) Design, synthesis and application of hydrogen bonded smectic liquid crystal matrix encapsulated ZnO nanospikes. J Mater Chem C 3(45):11907–11917

    CAS  Google Scholar 

  25. 25.

    Ghosh S et al (2012) Synthesis of silver nanoparticles using Dioscorea bulbifera tuber extract and evaluation of its synergistic potential in combination with antimicrobial agents. Int J Nanomedicine 7:483

    CAS  PubMed  PubMed Central  Google Scholar 

  26. 26.

    Ghosh S, Patil S, Ahire M, Kitture R, Gurav DD, Jabgunde AM, Kale S, Pardesi K, Shinde V, Bellare J, Dhavale DD, Chopade BA (2012) Gnidia glauca flower extract mediated synthesis of gold nanoparticles and evaluation of its chemocatalytic potential. J Nanobiotechnol 10(1):17

    CAS  Google Scholar 

  27. 27.

    Singh R, Shedbalkar UU, Wadhwani SA, Chopade BA (2015) Bacteriagenic silver nanoparticles: synthesis, mechanism, and applications. Appl Microbiol Biotechnol 99(11):4579–4593

    CAS  PubMed  Google Scholar 

  28. 28.

    Salunke GR et al (2014) Rapid efficient synthesis and characterization of silver, gold, and bimetallic nanoparticles from the medicinal plant Plumbago zeylanica and their application in biofilm control. Int J Nanomedicine 9:2635

    PubMed  PubMed Central  Google Scholar 

  29. 29.

    Shedbalkar U, Singh R, Wadhwani S, Gaidhani S, Chopade BA (2014) Microbial synthesis of gold nanoparticles: current status and future prospects. Adv Colloid Interf Sci 209:40–48

    CAS  Google Scholar 

  30. 30.

    Prasad KS, Selvaraj K (2014) Biogenic synthesis of selenium nanoparticles and their effect on As (III)-induced toxicity on human lymphocytes. Biol Trace Elem Res 157(3):275–283

    CAS  PubMed  Google Scholar 

  31. 31.

    Nancharaiah YV, Lens PN (2015) Selenium biomineralization for biotechnological applications. Trends Biotechnol 33(6):323–330

    CAS  PubMed  Google Scholar 

  32. 32.

    Baraka A, Dickson S, Gobara M, el-Sayyad GS, Zorainy M, Awaad MI, Hatem H, Kotb MM, Tawfic AF (2017) Synthesis of silver nanoparticles using natural pigments extracted from Alfalfa leaves and its use for antimicrobial activity. Chem Pap 71(11):2271–2281

    CAS  Google Scholar 

  33. 33.

    Goffeau A (2008) Drug resistance: the fight against fungi. Nature 452(7187):541–542

    CAS  PubMed  Google Scholar 

  34. 34.

    Marrs CF, Zhang L, Foxman B (2005) Escherichia coli mediated urinary tract infections: are there distinct uropathogenic E. coli (UPEC) pathotypes? FEMS Microbiol Lett 252(2):183–190

    CAS  PubMed  Google Scholar 

  35. 35.

    Kolodkin-Gal I, et al. (2012) A self-produced trigger for biofilm disassembly that targets exopolysaccharide. Cell 149(3):684–692

  36. 36.

    El-Batal AI et al (2019) Antibiofilm and Antimicrobial activities of silver boron nanoparticles synthesized by PVP polymer and gamma rays against urinary tract pathogens. J Clust Sci 30(4):947–964

    CAS  Google Scholar 

  37. 37.

    Singh R, Paul D, Jain RK (2006) Biofilms: implications in bioremediation. Trends Microbiol 14(9):389–397

    CAS  PubMed  PubMed Central  Google Scholar 

  38. 38.

    Parsek MR, Singh PK (2003) Bacterial biofilms: an emerging link to disease pathogenesis. Annu Rev Microbiol 57(1):677–701

    CAS  PubMed  Google Scholar 

  39. 39.

    Asmare M, Kebede M, Kebede A (2018) Prevalence of Uropathogenic Bactrial Profile, Antibiotic Susceptibility Patterns of Isolates and Assoiated Risk Factors among Patients of Urinary Tract Infection Visiting Haramaya Hospital, Estern Ethiopia. MSc Thesis, Haramaya University,Haramaya.

  40. 40.

    Ghanbari F, et al. (2017) An epidemiological study on the prevalence and antibiotic resistance patterns of bacteria isolated from urinary tract infections in central Iran. Avicenna J Clin Microbiol Infect 4(3):e42214.

  41. 41.

    El-Batal AI, Mosallam FM, El-Sayyad GS (2018) Synthesis of metallic silver nanoparticles by fluconazole drug and gamma rays to inhibit the growth of multidrug-resistant microbes. J Clust Sci 29(6):1003–1015

    CAS  Google Scholar 

  42. 42.

    El-Batal A et al (2014) Synthesis of silver nanoparticles and incorporation with certain antibiotic using gamma irradiation. Br J Pharmacol Res 4(11):1341–1363

    Google Scholar 

  43. 43.

    Gupta A, Saleh NM, Das R, Landis RF, Bigdeli A, Motamedchaboki K, Rosa Campos A, Pomeroy K, Mahmoudi M, Rotello VM (2017) Synergistic antimicrobial therapy using nanoparticles and antibiotics for the treatment of multidrug-resistant bacterial infection. Nano Futur 1(1):015004

    Google Scholar 

  44. 44.

    Bryaskova R, Pencheva D, Nikolov S, Kantardjiev T (2011) Synthesis and comparative study on the antimicrobial activity of hybrid materials based on silver nanoparticles (AgNps) stabilized by polyvinylpyrrolidone (PVP). J Chem Biol 4(4):185–191

    PubMed  PubMed Central  Google Scholar 

  45. 45.

    Balouiri M, Sadiki M, Ibnsouda SK (2016) Methods for in vitro evaluating antimicrobial activity: A review. Chin J Pharm Anal 6(2):71–79

    Google Scholar 

  46. 46.

    Attia MS, el-Sayyad GS, Saleh SS, Balabel NM, el-Batal AI (2019) Spirulina platensis-Polysaccharides promoted green silver nanoparticles production using gamma radiation to suppress the expansion of pear fire blight-producing Erwinia amylovora. J Clust Sci 30(4):919–935

    CAS  Google Scholar 

  47. 47.

    El-Batal AI et al (2018) Biogenic synthesis of copper nanoparticles by natural polysaccharides and Pleurotus ostreatus fermented fenugreek using gamma rays with antioxidant and antimicrobial potential towards some wound pathogens. Microb Pathog 118:159–169

    CAS  PubMed  Google Scholar 

  48. 48.

    Mosallam FM, el-Sayyad GS, Fathy RM, el-Batal AI (2018) Biomolecules-mediated synthesis of selenium nanoparticles using Aspergillus oryzae fermented Lupin extract and gamma radiation for hindering the growth of some multidrug-resistant bacteria and pathogenic fungi. Microb Pathog 122:108–116

    CAS  PubMed  Google Scholar 

  49. 49.

    Christensen GD, Simpson WA, Bisno AL, Beachey EH (1982) Adherence of slime-producing strains of Staphylococcus epidermidis to smooth surfaces. Infect Immun 37(1):318–326

    CAS  PubMed  PubMed Central  Google Scholar 

  50. 50.

    Ansari MA, Khan HM, Khan AA, Cameotra SS, Pal R (2014) Antibiofilm efficacy of silver nanoparticles against biofilm of extended spectrum β-lactamase isolates of Escherichia coli and Klebsiella pneumoniae. Appl Nanosci 4(7):859–868

    CAS  Google Scholar 

  51. 51.

    Maksoud MA et al (2019) Antibacterial, antibiofilm, and photocatalytic activities of metals-substituted spinel cobalt ferrite nanoparticles. Microb Pathog 127:144–158

    CAS  PubMed  Google Scholar 

  52. 52.

    Brownlee K (1952) Probit Analysis: a statistical treatment of the sigmoid response curve. Journal of the American Statistical Association 47(260):687–691

  53. 53.

    El-Batal A et al (2016) Impact of silver and selenium nanoparticles synthesized by gamma irradiation and their physiological response on early blight disease of potato. J Chem Pharm Res 8(4):934–951

    CAS  Google Scholar 

  54. 54.

    Kelly KL, et al. (2003) The optical properties of metal nanoparticles: the influence of size, shape, and dielectric environment. J Phys Chem B 107(3):668–677

  55. 55.

    El-Baz AF et al (2016) Extracellular biosynthesis of anti-Candida silver nanoparticles using Monascus purpureus. J Basic Microbiol 56(5):531–540

    CAS  PubMed  Google Scholar 

  56. 56.

    El-Batal AI et al (2012) Amelioration of oxidative damage induced in gamma irradiated rats by nano selenium and lovastatin mixture. World Appl Sci J 19(7):962–971

    CAS  Google Scholar 

  57. 57.

    Składanowski M et al (2017) Silver and gold nanoparticles synthesized from Streptomyces sp. isolated from acid forest soil with special reference to its antibacterial activity against pathogens. J Clust Sci 28(1):59–79

    Google Scholar 

  58. 58.

    Link S, El-Sayed MA (2003) Optical properties and ultrafast dynamics of metallic nanocrystals. Annu Rev Phys Chem 54(1):331–366

    CAS  PubMed  Google Scholar 

  59. 59.

    Liu F-K, Hsu YC, Tsai MH, Chu TC (2007) Using γ-irradiation to synthesize Ag nanoparticles. Mater Lett 61(11-12):2402–2405

    CAS  Google Scholar 

  60. 60.

    Granito M, Torres A, Frias J, Guerra M, Vidal-Valverde C (2005) Influence of fermentation on the nutritional value of two varieties of Vigna sinensis. Eur Food Res Technol 220(2):176–181

    CAS  Google Scholar 

  61. 61.

    Makarov V et al (2014) “Green” nanotechnologies: synthesis of metal nanoparticles using plants. Acta Naturae 6(1):20

    Google Scholar 

  62. 62.

    Zhao W (2010) A nucleotide-mediated strategy for the synthesis of bio-functionalized gold-nanoparticles. Hong Kong University of Science and Technology, Hong Kong

    Google Scholar 

  63. 63.

    Chen T, Wong Y (2008) Selenocystine induces apoptosis of A375 human melanoma cells by activating ROS-mediated mitochondrial pathway and p53 phosphorylation. Cell Mol Life Sci 65(17):2763–2775

    CAS  PubMed  Google Scholar 

  64. 64.

    Maksoud MA et al (2018) Synthesis and characterization of metals-substituted cobalt ferrite [MxCo(1-x) Fe2O4;(M = Zn, Cu and Mn; x = 0 and 0.5)] nanoparticles as antimicrobial agents and sensors for Anagrelide determination in biological samples. Mater Sci Eng C 92:644–656

    Google Scholar 

  65. 65.

    Ashour A et al (2018) Antimicrobial activity of metal-substituted cobalt ferrite nanoparticles synthesized by sol–gel technique. Particuology 40:141–151

    CAS  Google Scholar 

  66. 66.

    Maksoud MA et al (2019) Tunable structures of copper substituted cobalt nanoferrites with prospective electrical and magnetic applications. J Mater Sci Mater Electron 30(5):4908–4919

    CAS  Google Scholar 

  67. 67.

    Abdel Maksoud MIA, el-ghandour A, el-Sayyad GS, Awed AS, Ashour AH, el-Batal AI, Gobara M, Abdel-Khalek EK, el-Okr MM (2019) Incorporation of Mn2+into cobalt ferrite via sol–gel method: insights on induced changes in the structural, thermal, dielectric, and magnetic properties. J Sol-Gel Sci Technol 90(3):631–642

    CAS  Google Scholar 

  68. 68.

    Pal K, Sajjadifar S, Abd Elkodous M, Alli YA, Gomes F, Jeevanandam J, Thomas S, Sigov A (2019) Soft, self-assembly liquid crystalline nanocomposite for superior switching. Electron Mater Lett 15(1):84–101

    CAS  Google Scholar 

  69. 69.

    Bai K et al (2017) Preparation and antioxidant properties of selenium nanoparticles-loaded chitosan microspheres. Int J Nanomedicine 12:4527

    CAS  PubMed  PubMed Central  Google Scholar 

  70. 70.

    Belavi P et al (2012) Structural, electrical and magnetic properties of cadmium substituted nickel–copper ferrites. Mater Chem Phys 132(1):138–144

    CAS  Google Scholar 

  71. 71.

    Pal K, Elkodous MA, Mohan MM (2018) CdS nanowires encapsulated liquid crystal in-plane switching of LCD device. J Mater Sci Mater Electron 29(12):10301–10310

    CAS  Google Scholar 

  72. 72.

    El-Batal AI et al (2016) Biodiesel production by Aspergillus niger lipase immobilized on barium ferrite magnetic nanoparticles. Bioengineering 3(2):14

    PubMed Central  Google Scholar 

  73. 73.

    Elkodous MA et al (2019) Layer-by-layer preparation and characterization of recyclable nanocomposite (CoxNi1 − xFe2O4; X = 0.9/SiO2/TiO2). J Mater Sci Mater Electron 30(9):8312–8328

    CAS  Google Scholar 

  74. 74.

    El-Batal A et al (2013) Gamma irradiation induces silver nanoparticles synthesis by Monascus purpureus. J Chem Pharm Res 5(8):1–15

    Google Scholar 

  75. 75.

    El-Batal AI et al (2016) Physiological responses of two varieties of common bean (Phaseolus vulgaris L.) to foliar application of silver nanoparticles. Nanomater Nanotechnol 6:13

    Google Scholar 

  76. 76.

    El-Batal A et al (2014) Marine Streptomyces cyaneus strain Alex-SK121 mediated eco-friendly synthesis of silver nanoparticles using gamma radiation. Br J Pharmacol Res 4(21):2525–2547

    Google Scholar 

  77. 77.

    Hanora A et al (2016) J Chem Pharm Res 8(3):405–423

    CAS  Google Scholar 

  78. 78.

    Phanjom P, Ahmed G (2015) Biosynthesis of silver nanoparticles by Aspergillus oryzae (MTCC No. 1846) and its characterizations. Nanosci Nanotechnol 5(1):14–21

    CAS  Google Scholar 

  79. 79.

    Ballottin D, Fulaz S, Souza ML, Corio P, Rodrigues AG, Souza AO, Gaspari PM, Gomes AF, Gozzo F, Tasic L (2016) Elucidating protein involvement in the stabilization of the biogenic silver nanoparticles. Nanoscale Res Lett 11(1):313

    PubMed  PubMed Central  Google Scholar 

  80. 80.

    Dwivedi C, Pandey H, Pandey A, Ramteke P (2015) Fabrication and assessment of gentamicin loaded electrospun nanofibrous scaffolds as a quick wound healing dressing material. Curr Nanosci 11(2):222–228

    CAS  Google Scholar 

  81. 81.

    Pandey H, Parashar V, Parashar R, Prakash R, Ramteke PW, Pandey AC (2011) Controlled drug release characteristics and enhanced antibacterial effect of graphene nanosheets containing gentamicin sulfate. Nanoscale 3(10):4104–4108

    CAS  PubMed  Google Scholar 

  82. 82.

    Palanikumar S, Meenarathi B, Anbarasan R (2014) Synthesis, characterization and applications of gentamicin functionalized Fe3O4 nano hybrid. International Journal of Emerging Technologies and Engineering (IJETE) 9-14

  83. 83.

    Saravanan M, Gopinath V, Chaurasia MK, Syed A, Ameen F, Purushothaman N (2018) Green synthesis of anisotropic zinc oxide nanoparticles with antibacterial and cytofriendly properties. Microb Pathog 115:57–63

    CAS  PubMed  Google Scholar 

  84. 84.

    Arakelova ER et al (2014) In vitro and in vivo anticancer activity of nanosize zinc oxide composites of doxorubicin. Int J Med Heal Pharm Biomed Eng 8:33–38

    Google Scholar 

  85. 85.

    Dagmar H, et al. (2015) Selenium nanoparticles and evaluation of their antimicrobial activity on bacterial isolates obtained from clinical specimens. Nanocon

  86. 86.

    Tang Z-X, Lv B-F (2014) MgO nanoparticles as antibacterial agent: preparation and activity. Braz J Chem Eng 31(3):591–601

    Google Scholar 

  87. 87.

    Pal S, Tak YK, Song JM (2007) Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the gram-negative bacterium Escherichia coli. Appl Environ Microbiol 73(6):1712–1720

    CAS  PubMed  PubMed Central  Google Scholar 

  88. 88.

    Ojo SA, Lateef A, Azeez MA, Oladejo SM, Akinwale AS, Asafa TB, Yekeen TA, Akinboro A, Oladipo IC, Gueguim-Kana EB, Beukes LS (2016) Biomedical and catalytic applications of gold and silver-gold alloy nanoparticles biosynthesized using cell-free extract of Bacillus safensis LAU 13: antifungal, dye degradation, anti-coagulant and thrombolytic activities. IEEE Trans Nanobiosci 15(5):433–442

    Google Scholar 

  89. 89.

    Lateef A, Ojo SA, Folarin BI, Gueguim-Kana EB, Beukes LS (2016) Kolanut (Cola nitida) mediated synthesis of silver–gold alloy nanoparticles: antifungal, catalytic, larvicidal and thrombolytic applications. J Clust Sci 27(5):1561–1577

    CAS  Google Scholar 

  90. 90.

    Lateef A, Azeez MA, Asafa TB, Yekeen TA, Akinboro A, Oladipo IC, Azeez L, Ojo SA, Gueguim-Kana EB, Beukes LS (2016) Cocoa pod husk extract-mediated biosynthesis of silver nanoparticles: its antimicrobial, antioxidant and larvicidal activities. J Nanostruct Chem 6(2):159–169

    CAS  Google Scholar 

  91. 91.

    He Y, Chen S, Liu Z, Cheng C, Li H, Wang M (2014) Toxicity of selenium nanoparticles in male Sprague–Dawley rats at supranutritional and nonlethal levels. Life Sci 115(1-2):44–51

    CAS  PubMed  Google Scholar 

  92. 92.

    Azab KS et al (2015) Nano selenium-lovastatin mixture modulate inflammatory cascade in arthritic irradiated model. Int J Radiat Res 13(4):305–316

    Google Scholar 

  93. 93.

    Shakibaie M, Shahverdi AR, Faramarzi MA, Hassanzadeh GR, Rahimi HR, Sabzevari O (2013) Acute and subacute toxicity of novel biogenic selenium nanoparticles in mice. Pharm Biol 51(1):58–63

    CAS  PubMed  Google Scholar 

  94. 94.

    Cremonini E, Zonaro E, Donini M, Lampis S, Boaretti M, Dusi S, Melotti P, Lleo MM, Vallini G (2016) Biogenic selenium nanoparticles: characterization, antimicrobial activity and effects on human dendritic cells and fibroblasts. Microb Biotechnol 9(6):758–771

    CAS  PubMed  PubMed Central  Google Scholar 

  95. 95.

    Ashajyothi C, Harish KH, Dubey N, Chandrakanth RK (2016) Antibiofilm activity of biogenic copper and zinc oxide nanoparticles-antimicrobials collegiate against multiple drug resistant bacteria: a nanoscale approach. J Nanostruct Chem 6(4):329–341

    CAS  Google Scholar 

  96. 96.

    Park H-J, Kim HY, Cha S, Ahn CH, Roh J, Park S, Kim S, Choi K, Yi J, Kim Y, Yoon J (2013) Removal characteristics of engineered nanoparticles by activated sludge. Chemosphere 92(5):524–528

    CAS  PubMed  Google Scholar 

  97. 97.

    Stoimenov PK, Klinger RL, Marchin GL, Klabunde KJ (2002) Metal oxide nanoparticles as bactericidal agents. Langmuir 18(17):6679–6686

    CAS  Google Scholar 

  98. 98.

    Khan MF, Ansari AH, Hameedullah M, Ahmad E, Husain FM, Zia Q, Baig U, Zaheer MR, Alam MM, Khan AM, AlOthman ZA, Ahmad I, Ashraf GM, Aliev G (2016) Sol-gel synthesis of thorn-like ZnO nanoparticles endorsing mechanical stirring effect and their antimicrobial activities: Potential role as nano-antibiotics. Sci Rep 6:27689

    CAS  PubMed  PubMed Central  Google Scholar 

  99. 99.

    Shinde A, Ganu J, Naik P (2012) Effect of free radicals & antioxidants on oxidative stress: a review. J Dent Allied Sci 1(2):63

    Google Scholar 

  100. 100.

    Wang Q (2015) Nanosized selenium: a novel platform technology to prevent bacterial infections, PhD THESIS in the field of Bioengineering, Northeastern University Boston, Massachusetts

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The authors acknowledge the P.I. of Nanotechnology Research Unit (Prof. Dr. Ahmed I. El-Batal) for financing and supporting this study under the project “Nutraceuticals and Functional Foods Production by Using Nano/Biotechnological and Irradiation Processes.” Also, the authors would like to thank Dr. Muhammed I. Abdel Maksoud (Lecturer at NCRRT) and Zeiss microscope team at Cairo, Egypt, for their invaluable support of this study.

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El-Sayyad, G.S., El-Bastawisy, H.S., Gobara, M. et al. Gentamicin-Assisted Mycogenic Selenium Nanoparticles Synthesized Under Gamma Irradiation for Robust Reluctance of Resistant Urinary Tract Infection-Causing Pathogens. Biol Trace Elem Res 195, 323–342 (2020).

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  • Selenium nanoparticles
  • Gentamicin
  • Gamma irradiation
  • E. coli
  • Penicillium chrysogenum
  • Urinary tract pathogens