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Liver Proteome of Mice with Distinct Genetic Susceptibilities to Fluorosis Treated with Different Concentrations of F in the Drinking Water

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Abstract

Appropriate doses of fluoride (F) have therapeutic action against dental caries, but higher levels can cause disturbances in soft and mineralized tissues. Interestingly, the susceptibility to the toxic effects of F is genetically determined. This study evaluated the effects of F on the liver proteome of mice susceptible (A/J) or resistant (129P3/J) to the effects of F. Weanling male A/J (n = 12) and 129P3/J (n = 12) mice were housed in pairs and assigned to two groups given low-F food and drinking water containing 15 or 50 ppm F for 6 weeks. Liver proteome profiles were examined using nano-LC-ESI-MS/MS. Difference in expression among the groups was determined using the PLGS software. Treatment with the lower F concentration provoked more pronounced alterations in fold change in liver proteins in comparison to the treatment with the higher F concentration. Interestingly, most of the proteins with fold change upon treatment with 15 ppm F were increased in the A/J mice compared with their 129P3/J counterparts, suggesting an attempt of the former to fight the deleterious effects of F. However, upon treatment with 50 ppm F, most proteins with fold change were decreased in the A/J mice compared with their 129P3/J counterparts, especially proteins related to oxidative stress and protein folding, which might be related to the higher susceptibility of the A/J animals to the deleterious effects of F. Our findings add light into the mechanisms underlying genetic susceptibility to fluorosis.

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Acknowledgements

The authors thank CNPq/TWAS for providing a scholarship to the first author (190145/2013-7).

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Authors and Affiliations

  1. Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Al. Octávio Pinheiro Brisolla, 9-75, Bauru, São Paulo, 17012-901, Brazil

    Zohaib Nisar Khan, Isabela Tomazini Sabino, Carina Guimarães de Souza Melo, Tatiana Martini, Heloísa Aparecida Barbosa da Silva Pereira & Marília Afonso Rabelo Buzalaf

  2. Department of Genetics and Evolution, Center of Biological Sciences and the Health, Federal University of São Carlos, Washington Luis, Km 235, São Carlos, São Paulo, 13560-970, Brazil

    Heloísa Aparecida Barbosa da Silva Pereira

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  1. Zohaib Nisar Khan
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  2. Isabela Tomazini Sabino
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  3. Carina Guimarães de Souza Melo
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Correspondence to Marília Afonso Rabelo Buzalaf.

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Khan, Z.N., Sabino, I.T., de Souza Melo, C.G. et al. Liver Proteome of Mice with Distinct Genetic Susceptibilities to Fluorosis Treated with Different Concentrations of F in the Drinking Water. Biol Trace Elem Res 187, 107–119 (2019). https://doi.org/10.1007/s12011-018-1344-8

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