Abstract
Fluoride (F) is known to induce reproduction toxicity, and the elucidation of its underlying mechanisms is an ongoing research. These findings aim to provide deeper insights into roles of soduim fluoride (NaF) in testis damage, which could contribute to a better understanding of fluoride-induced male reproductive toxicity. The Leydig cells were administrated by 0, 5, 10, and 20 mg/L NaF for 24 h, respectively. Scanning electron microscope was used to identify the change of surface structure in the Leydig cells. The results showed that fluoride exposure of high-dose induced bulged balloon in the membrane of the Leydig cell. We speculated that high doses of NaF inhibited cell proliferation and induced cell apoptosis in Leydig cells. This is the first time that we have reported that fluoride impaired cytomembrane and cytoskeleton of the Leydig cells in vitro treated with different doses of fluoride for 24 h by using a scanning electron microscope. Damage to the cytoskeleton and cytomembrane may be one of the reasons of male reproductive toxicity induced by fluoride.
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References
Li Y, Bian S, Wang J, Wang J (2016) Effects of fluoride and chitosan on the gene expressions of bone morphogenic protein 2 and collagen type-1 alpha1 chain in the mouse femur. Fluoride 49:47–55
Wan SX, Zhang JH, Wang JD (2006) Effects of high fluoride on sperm quality and testicular histology in male rats. Fluoride 39:17–21
Zhang S, Jiang C, Liu H, Guan Z, Zeng Q, Zhang C, Lei R, Xia T, Gao H, Yang L, Chen Y, Wu X, Zhang X, Cui Y (2013) Fluoride-elicited developmental testicular toxicity in rats: roles of endoplasmic reticulum stress and inflammatory response. Toxicol Appl Pharmacol 271:206–215
Yan X, Hao X, Nie Q, Feng C, Wang H, Sun Z, Niu R, Wang J (2015) Effects of fluorifde on the ultrastructure and expression of Type I collagen in rat hard tissue. Chemosphere 128:36–41
Zhou BH, Zhao J, Liu J, Zhang JL, Li J, Wang HW (2015) Fluoride-induced oxidative stress is involved in the morphological damage and dysfunction of liver in female mice. Chemosphere 139:504–511
Xu H, Hu LS, Chang M, Jing L, Zhang XY, Li GS (2005) Proteomic analysis of kidney in fluoride-treated rat. Toxicol Lett 160:69–75
Niu R, Liu S, Wang J, Zhang J, Sun Z, Wang J (2014) Proteomic analysis of hippocampus in offspring male mice exposed to fluoride and lead. Biol Trace Elem Res 162:227–233
Ge Y, Niu R, Zhang J, Wang J (2011) Proteomic analysis of brain proteins of rats exposed to high fluoride and low iodine. Arch Toxicol 85:27–33
Zhou B, Wang H, Wang J, Zhang J, Yan X, Wang J (2009) Effects of malnutrition and supplemented nutrition on non-specific immune function changes induced by fluoride in rabbits. Fluoride 40:169–177
Barbier O, Arreola-Mendoza L, Del Razo LM (2010) Molecular mechanisms of fluoride toxicity. Chem Biol Interact 188:319–333
Su K, Sun Z, Niu R, Lei Y, Cheng J, Wang J (2017) Cell cycle arrest and gene expression profiling of testis in mice exposed to fluoride. Environ Toxicol 32:1558–1565
Cao J, Chen Y, Chen J, Yan H, Li M, Wang J (2016) Fluoride exposure changed the structure and the expressions of Y chromosome related genes in testes of mice. Chemosphere 161:292–299
Guan Y, Hao P, Tang C, Li P, Li S, Cheng X, Ba Y, Cui L (2012) Effect of fluoride on the expression of StAR mRNA and P450scc mRNA in the testosterone synthesis of mouse Leydig tumor cells. Wei Sheng Yan Jiu 41:105–108
Zhang J, Liang C, Ma J, Niu R, Wang J (2006) Effects of sodium fluoride and sulfur dioxide on sperm motility and serum testosterone in male rats. Fluoride 39:126–131
Sun Z, Niu R, Su K, Wang J, Zhang J, Wang J (2010) Effects of sodium fluoride on hyperactivation and Ca2+ signaling pathway in sperm from mice: an in vivo study. Arch Toxicol 84:353–361
Song GH, Wang RL, Chen ZY, Zhang B, Wang HL, Liu ML, Gao JP, Yan XY (2014) Toxic effects of sodium fluoride on cell proliferation and apoptosis of Leydig cells from young mice. J Physiol Biochem 70:761–768
Gupta RS, Khan TI, Agrawal D, Kachhawa JB (2007) The toxic effects of sodium fluoride on the reproductive system of male rats. Toxicol Ind Health 23:507–513
Yu Y, Han Y, Niu R, Wang J, Manthari RK, Ommati MM, Sun Z (2017) Ameliorative effect of VE, IGF-I, and hCG on the fluoride-induced testosterone release suppression in mice Leydig cells. Biol Trace Elem Res. doi:10.1007/s12011-017-1023-1
Payne AH, Downing JR, Wong KL (1980) Luteinizing hormone receptors and testosterone synthesis in two distinct populations of Leydig cells. Endocrinology 106:1424–1429
Trevors JT, Jr MHS (2009) Where is the global environmental bailout? Water Air Soil Pollut 198:1–3
WHO (2011) Guidelines for drinking water quality 4th ed. World Health Organ 1:178
Kumar N, Sood S, Arora B, Singh M, Beena (2010) Effect of duration of fluoride exposure on the reproductive system in male rabbits. J Hum Reprod Sci 3:148–152
Panneerselvam L, Raghunath A, Perumal E (2017) Acute fluoride poisoning alters myocardial cytoskeletal and AMPK signaling proteins in rats. Int J Cardiol. doi:10.1016/j.ijcard.2016.11.221
Ratanachoo K, Gascoyne PR, Ruchirawat M (2002) Detection of cellular responses to toxicants by dielectrophoresis. Biochim Biophys Acta 1564:449–458
Chen M, Wang J (2002) Initiator caspases in apoptosis signaling pathways. Apoptosis 7:313–319
Tatin F, Grise F, Reuzeau E, Genot E, Moreau V (2010) Sodium fluoride induces podosome formation in endothelial cells. Biol Cell 102:489–498
Chen L, Ning H, Yin Z, Song X, Feng Y, Qin H, Li Y, Wang J, Ge Y, Wang W (2017) The effects of fluoride on neuronal function occurs via cytoskeleton damage and decreased signal transmission. Chemosphere. doi:10.1016/j.chemosphere.2017.06.128
Yang L, Tang L, Dai F, Meng G, Yin R, Xu X, Yao W (2017) Raf-1/CK2 and RhoA/ROCK signaling promote TNF-α-mediated endothelial apoptosis via regulating vimentin cytoskeleton. Toxicology. doi:10.1016/j.tox.2017.07.010
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We thank Ram Kumar Manthari for the improvement in English syntax and word usage.
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All regulations of the Institutional Animal Care and Use Committee of Shanxi Agricultural University were strictly followed during the experiment to protect the welfare of the animals.
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This research was supported by the National Natural Science Foundation of China (Grant No.31172380).
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Li, J., Shi, Y., Fan, H. et al. Effects of Fluoride on Surface Structure of Primary Culture Leydig Cells in Mouse. Biol Trace Elem Res 183, 123–127 (2018). https://doi.org/10.1007/s12011-017-1121-0
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DOI: https://doi.org/10.1007/s12011-017-1121-0