Biological Trace Element Research

, Volume 176, Issue 2, pp 305–310 | Cite as

Estimation of Mineral and Trace Element Profile in Bubaline Milk Affected with Subclinical Mastitis

  • Mahavir Singh
  • Poonam Yadav
  • Anshu Sharma
  • V.K. Garg
  • Dinesh Mittal


The milk samples from buffaloes of Murrah breed at mid lactation stage, reared at an organised dairy farm, were screened for subclinical mastitis based on bacteriological examination and somatic cell count following International Dairy Federation criteria. Milk samples from subclinical mastitis infected and healthy buffaloes were analysed to evaluate physicochemical alterations in terms of protein, fat, pH, electrical conductivity, chloride, minerals (sodium, potassium and calcium) and trace elements (iron, zinc, copper and selenium). In the present study, protein, fat, zinc, iron, calcium and selenium content was significantly lower (P < 0.001), while pH and electrical conductivity were significantly higher in mastitic milk as compared to normal milk. Concentration of electrolytes mainly sodium and chloride significantly increased with higher somatic cell count in mastitic milk and to maintain osmolality; potassium levels decreased proportionately. Correlation matrix revealed significantly positive interdependences of somatic cell count with pH, electrical conductivity, sodium and chloride. However, protein, fat, calcium and potassium were correlated negatively with elevated somatic cell count in mastitic milk. It is concluded that udder infections resulting in elevated somatic cells may alter the mineral and trace element profile of milk, and magnitude of changes may have diagnostic and prognostic value.


Subclinical mastitis Compositional quality Trace elements Minerals Buffalo milk Somatic cell counts 



Authors are thankful to Center for Radioecology, Guru Jambheshwar University of Science and Technology, Hisar, India, for providing the analysis facilities. Author MS acknowledges the financial assistance given by the Department of Science and Technology, Govt. of India in the form of INSPIRE fellowship for pursuing doctoral programme.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflicts of interest.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Mahavir Singh
    • 1
  • Poonam Yadav
    • 2
  • Anshu Sharma
    • 1
  • V.K. Garg
    • 3
  • Dinesh Mittal
    • 1
  1. 1.College Central LabLala Lajpat Rai University of Veterinary and Animal SciencesHisarIndia
  2. 2.Centre for Radio-ecologyGuru Jambheshwar University of Science and TechnologyHisarIndia
  3. 3.Centre for Environmental Sciences and TechnologyCentral University of PunjabBathindaIndia

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