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Biological Trace Element Research

, Volume 176, Issue 2, pp 328–337 | Cite as

Selenium Ameliorate Peripheral Nerve Ischemic-Reperfusion Injury via Decreased TNF-α

  • Abolfazl Zendedel
  • Zahra Gharibi
  • Khatereh Anbari
  • Abolfazl Abbaszadeh
  • Zahra Khanipour khayat
  • Reza Mohammadrezaei Khorramabadi
  • Maryam Soleymaninejad
  • Mohammadreza Gholami
Article

Abstract

Selenium is considered as a trace element that plays antioxidant role in the body. So, the aim of this study was to evaluate the effect of selenium on ameliorating of sciatic nerve ischemia-reperfusion injury. Eighty (80) adult male Wistar rats weighing 250–300 g were used. They were divided into 10 groups (n = 8). Then, femoral vessels were obstructed by using 4/0 silk and splitknot techniques. After 3-h ischemia for all the groups, reperfusion was applied for different periods: 3, 7, 14, and 28 days. In half of each experimental group, 0.2 mg/kg selenium was injected intraperitoneally, coinciding with ischemia. After reperfusion, according to the grouping, rats were killed by using high dose of anesthetic drug and then sciatic nerve was removed and fixed. Then, tissue samples were processed and subsequently stained with hematoxylin-eosin, apoptosis, and immunohistochemistry stains. On the third day of reperfusion, the amount of TNF-α as an inflammatory marker of ischemia-reperfusion acute phase increased. On the seventh day of reperfusion, the amount of NF-кB as an apoptotic index and infiltration of mast cells increased in the tissue as a result of development of inflammation. But, on the 14th day of reperfusion, the amount of NF-кB as an apoptotic index decreased to the lowest amount. On the 28th day of reperfusion, the amount of TNF-α as an inflammatory marker decreased to its lowest level. Prescription of selenium concurrent with development of ischemia can reduce the damage caused by sciatic nerve ischemia-reperfusion.

Keywords

Selenium Ischemia Sciatic nerve TNF-α 

Notes

Acknowledgments

This study was approved in the Lorestan University of Medical Sciences. Special thanks to Lorestan University of Medical Sciences for the financial support, Khorramabad, Iran. The authors thank the head and staff of Razi Herbal Drugs Research Center of Lorestan Medical University.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interests.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Abolfazl Zendedel
    • 1
    • 2
  • Zahra Gharibi
    • 3
  • Khatereh Anbari
    • 4
  • Abolfazl Abbaszadeh
    • 5
  • Zahra Khanipour khayat
    • 5
  • Reza Mohammadrezaei Khorramabadi
    • 6
  • Maryam Soleymaninejad
    • 7
  • Mohammadreza Gholami
    • 1
    • 3
  1. 1.Razi Herbal Medicines Research CenterLorestan University of Medical SciencesKhorramabadIran
  2. 2.Department of Internal MedicineLorestan University of Medical SciencesKhorramabadIran
  3. 3.Department of Anatomical SciencesLorestan University of Medical SciencesKhorramabadIran
  4. 4.Department of Social MedicineLorestan University of Medical SciencesKhorramabadIran
  5. 5.Department of SurgeryLorestan University of Medical SciencesKhorramabadIran
  6. 6.Student Research CommitteeLorestan University of Medical SciencesKhorramabadIran
  7. 7.Faculty of MedicineBabol University of Medical SciencesBabolIran

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