Antidiabetic Activity of a Lotus Leaf Selenium (Se)-Polysaccharide in Rats with Gestational Diabetes Mellitus
A selenium (Se)-containing polysaccharide, lotus leaf selenium (Se)-polysaccharide (LLP), was isolated from a lotus leaf. The effects of LLP on antioxidant enzyme activities and insulin resistance in pregnant rats with gestational diabetes mellitus (GDM) were investigated. LLP administered orally at two doses (50 and 100 mg/kg) could significantly reverse the weight loss of pregnant rats before the delivery, fetal rats, and placentas in GDM rats (P < 0.05). Furthermore, LLP treatment induced a decrease of fasting blood glucose (FBG) and fasting blood insulin (FINS) levels in GDM rats, but an increase of hepatic glycogen content, when compared with those in GDM rats (P < 0.05). Also, oral administrations of LLP markedly improved the lipid profile of GDM rats, as evidenced by a reduction of total cholesterol (TC), triglyceride (TG), and low-density lipoprotein (LDL) cholesterol levels except for the high-density lipoprotein (HDL) cholesterol level. Additionally, antioxidant enzyme levels, such as superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), and glutathione (GSH), in liver tissues of the GDM group were lower than those of the other groups, and following treatment of LLP, these indexes in liver tissues were equivalent to those of the control group (P > 0.05). All the data indicated that LLP may be a promising drug candidate or a healthcare food for GDM therapy or protection.
KeywordsLotus leaf selenium (Se)-polysaccharide (LLP) Gestational diabetes mellitus Oxidative damage
Compliance with Ethical Standards
All of the experimental procedures were conducted strictly in conformity with the NIH Guide for the Care and Use of Laboratory Animals and approved by the Animal Care Committee of Xinxiang Medical University.
Conflict of Interest
The authors declare that they have no competing interests.
All institutional and national guidelines for the care and use of laboratory animals were followed.
- 3.Schaefer-Graf UM, Klavehn S, Hartmann R, Kleinwechter H, Demandt N, Sorger M, Kjos SL, Vetter K, Abou-Dakn M (2009) How do we reduce the number of cases of missed postpartum diabetes in women with recent gestational diabetes mellitus? Diabetes Care 32:1960–1964CrossRefPubMedPubMedCentralGoogle Scholar
- 10.Kinnunen TI, Puhkala J, Raitanen J, Ahonen S, Aittasalo M, Virtanen SM, Luoto R (2014) Effects of dietary counselling on food habits and dietary intake of Finnish pregnant women at increased risk for gestational diabetes—a secondary analysis of a cluster-randomized controlled trial. Matern Child Nutr 10:184–197CrossRefPubMedGoogle Scholar
- 23.Zhou T, Luo D, Li XY, Luo Y (2009) Hypoglycemic and hypolipidemic effects of flavonoids from lotus (Nelumbo nuficera Gaertn) leaf in diabetic mice. J Med Plant Res 3:290–293Google Scholar
- 24.Kim AR1, Jeong SM, Kang MJ, Jang YH, Choi HN, Kim JI (2013) Lotus leaf alleviates hyperglycemia and dyslipidemia in animal model of diabetes mellitus. Nutr Res Pract 7: 166–171.Google Scholar
- 25.Staub AM (1965) Removal of protein-Sevag method. Methods Carbohydr Chem 5:5–6Google Scholar
- 34.Buccolo G, David M (1973) Quantitative determination of serum triglycerides by use of enzyme. Clin Chem 19:476–482Google Scholar
- 37.Liu G, Zhao L, Liu H, Kang J (2008) Experimental study on streptozotocin induced diabetes in pregnancy affect fetal development. China Journal of Pediatrics 1:26–30Google Scholar