Kinetics of Tissue Iron in Experimental Autoimmune Encephalomyelitis in Rats
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To elucidate the role of iron in the pathomechanisms of autoimmune CNS disorders, we estimated the tissue concentrations of Fe2+ in the brain, spinal cord, and liver in the chronic relapsing form of experimental autoimmune encephalomyelitis (EAE). The disease was induced in Dark Agouti (DA) strain of rats, by subcutaneous injection of bovine brain homogenate in complete Freund's adjuvant (CFA). Control rats consisted of unsensitized rats and of rats treated with CFA or saline. The data obtained by clinical assessment and by inductively coupled plasma spectrometry have shown that the attacks of disease (on the 12th and 22nd post-immunization day) were followed by high accumulation of iron in the liver. Additionally, during the second attack of disease, the decreased concentration of Fe2+ was found in cervical spinal cord. The data point to regulatory effects of iron and hepatic trace elements regulating mechanisms in the pathogenesis of EAE.
KeywordsChronic relapsing EAE DA rats Inductivity coupled plasma spectrometry Iron Brain Spinal cord Liver
This work was supported by a grant from the Croatian Ministry of Science (Projects Nos 62-0621341-1337 and 62-0621341-0061).
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