This study evaluated the physico-chemical, antimicrobial, and organoleptic properties of roasted clove bud in cake preservation during 28 days of storage at room temperature (30°C) and compared with cake containing synthetic antioxidant. The results showed that the clove as such and in roasted form was able to retard the oxidative products in cakes when compared with synthetic antioxidant. Clove as such and in roasted form was more effective as antimicrobial agent as evident from the growth of the tested microorganisms. The cake containing unroasted and roasted clove bud powder showed more antioxidant properties compared with cake containing synthetic antioxidant. The pattern of chemical composition of clove bud oil as such and roasted by hot plate and microwave was responsible for more stability of cake when compared with cake containing synthetic antioxidant. Also, sensory results indicated in general the acceptability of the different cake samples. Color was observed to be more intense in case of roasted samples. Roasted samples indicated more hardness due to change in morphology by heating. Clove bud with intact essential oil as such and its roasted form could be used as a natural antioxidant and antimicrobial agent in foodstuffs, particularly those containing lipid and can help to extend the shelf life of those food products, besides their health safety.
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Kappus, H., & Kahl, R. (1993). Toxicology of the Synthetic Antioxidants BHA and BHT in Comparison with Natural Antioxidant Vitamin E. Zeitschriftfur, Lebensmittel-Untersuchung Forshung, 196, 329–338. https://doi.org/10.1007/BF01197931.
Gülçin, I. (2011). Antioxidant activity of eugenol: a structure–activity relationship study. Journal of Medicinal Food, 14(9), 975–985. https://doi.org/10.1089/jmf.2010.0197.
Pérez-Jiménez, J., Neveu, V., Vos, F., & Scalbert, A. (2010). Identification of the 100 richest dietary sources of polyphenols: an application of the phenol-explorer database. European Journal of Clinical Nutrition, 64(S3), S112–S120. https://doi.org/10.1038/ejcn.2010.221.
Wu, S., Metcalf, J. P., & Wu, W. (2010). Protective essential oil attenuates influenza virus infection in MDCK cells. B25. H1N1, Seasonal Influenza and Other Viral Pneumonia: Clinical and Mechanistic Insights. American Journal of Respiratory and Critical Care Medicine, 181, A2639. https://doi.org/10.1164/ajrccm-conference.2010.181.1_meetingabstracts.a2639.
Lu, T., Lee, C., Mau, J., & Lin, S. (2010). Quality and antioxidant property of green tea sponge cake. Food Chemistry, 119(3), 1090–1095.
Morales, F., & van Boekel, M. (1998). A study on advanced Maillard reaction in heated casein/sugar solutions: colour formation. International Dairy Journal, 8(10-11), 907–915.
Chakraborty, P., Chakraborty, N., Bhattacharyya, D. K., & Ghosh, M. (2016). Effect of tamarind kernel powder incorporation in property and quality aspects of biscuit, bread and cake making. Archives of Applied Science Research, 8(1), 30–39.
Chakraborty, P., Sahoo, S., Bhattacharyya, D. K., & Ghosh, M. (2020). Marine lizardfish (Harpadon nehereus) meal concentrate in preparation of ready-to-eat protein and calcium rich extruded snacks. Journal of Food Science and Technology, 57(1), 338–349.
Baiano, A., & Del Nobile, M. (2005). Shelf life extension of almond paste pastries. Journal of Food Engineering, 66(4), 487–495.
AOCS, Official Method Ca 5a–40. (1997). Free fatty acids. Sampling and analysis of commercial fats and oils. Journal of the American Oil Chemists’ Society.
AOCS, Official Method Cd 8–53. (1997). Peroxide value acetic acid- chloroform method. Sampling and analysis of commercial fats and oils. Journal of the American Oil Chemists’ Society.
AOCS, Official Method Cd 18–90. (1997). p-Anisidine value. Sampling and analysis of commercial fats and oils. Journal of the American Oil Chemists’ Society.
Patton, S., & Kurtz, G. (1951). 2-thiobarbituric acid as a reagent for detecting milk fat oxidation. Journal of Dairy Science, 34(7), 669–674.
Singleton, V. L., Orthofer, R., & Lamuela Raventós, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology, 299, 152–178.
Shimada, K., Fujikawa, K., Yahara, K., & Nakamura, T. (1992). Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. Journal of Agricultural and Food Chemistry, 40(6), 945–948.
Benzie, I., & Strain, J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Analytical Biochemistry, 239(1), 70–76.
Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9-10), 1231–1237.
Amin, T., & Thakur, M. (2014). A comparative study on proximate composition, phytochemical screening, antioxidant and antimicrobial activities of Linum usitatisimum L. (flaxseeds). Int.J.Curr.Microbiol. App.Sci, 3(4), 465–481.
Applewhite, H. T. (1985). Bailey’s industrial oil and fat products (1st ed.p. 93). New York: Wiley.
Heenan, S., Dufour, J., Hamid, N., Harvey, W., & Delahunty, C. (2010). The influence of ingredients and time from baking on sensory quality and consumer freshness perceptions in a baked model cake system. LWT - Food Science and Technology, 43(7), 1032–1041.
Ibrahium, M. I., Abd El-Ghany, M. E., & Ammar, M. S. (2013). Effect of clove essential oil as antioxidant and antimicrobial agent on cake shelf life. World Journal of Dairy & Food Sciences, 8(2), 140–146. https://doi.org/10.5829/idosi.wjdfs.2013.8.2.7633.
Dev, M., Bhattacharyya, D. K., & Ghosh, M. (2021). Composition and antioxidant activity of vapours from clove bud during roasting. In D. Ramkrishna, S. Sengupta, S. Dey Bandyopadhyay, & A. Ghosh (Eds.), Advances in Bioprocess Engineering and Technology. Lecture Notes in Bioengineering (pp. 151–158). Singapore: Springer.
Chan-Wei, Y., Li, W.-H., Hsu, F.-L., Yen, P.-L., Chang, S.-T., & Liao, V. H.-C. (2014). Essential oil alloaromadendrene from mixed-type Cinnamomum osmophloeum leaves prolongs the lifespan in Caenorhabditis elegans. Journal of Agricultural and Food Chemistry, 62(26), 6159–6165.
Yi, S. M., Zhu, J. L., Fu, L. L., & Li, J. R. (2010). Tea polyphenols inhibit Pseudomonas aeruginosa through damage to the cell membrane. International Journal of Food Microbiology, 144, 111–117.
The School of Community Science and Technology (SOCSAT),Indian Institute of Engineering Science and Technology (IIEST), Shibpur, Howrah, West Bengal, India, is acknowledged for offering research facilities.
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Dev, M., Ghosh, M. & Bhattacharyya, D.k. Physico-chemical, Antimicrobial, and Organoleptic Properties of Roasted Aromatic Spice (Clove Bud) in Baked Product. Appl Biochem Biotechnol (2021). https://doi.org/10.1007/s12010-021-03504-0
- Clove bud