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Optimization of Initial Cation Concentrations for L-Lactic Acid Production from Fructose by Lactobacillus pentosus Cells

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Abstract

In this study, Box-Behnken design was applied to optimize the initial concentrations of 4 cations for L-lactic acid production from fructose by homologous batch fermentation of Lactobacillus pentosus cells. The optimum initial cation concentrations were obtained as 6.542 mM Mg2+, 3.765 mM Mn2+, 2.397 mM Cu2+, and 3.912 mM Fe2+, respectively. The highest L-lactic acid yield and productivity were obtained as 0.935 ± 0.005 g/g fructose and 1.363 ± 0.021 g/(L × h), respectively, with a maximum biomass concentration of 7.97 ± 0.17 g/L. The effectiveness of the optimization by Box-Behnken design was confirmed based on the small errors between predicted results and experimental results shown as 0.3%, − 0.2%, and − 1.2%, respectively. The quadratic models with high accuracy and reliability can be applied to mathematically forecasted the fermentation performance. After the optimization, the lactic acid yield and productivity were significantly improved by 3.7% and 21.0%, respectively.

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Data Availability

All data and materials are available.

Abbreviations

ANOVA:

Analysis of variance

ATCC:

American Type Culture Collection

DOE:

Design of experiment

EMP:

EmbdenMeyerhoff-Parnas

LA:

Lactic acid

LAB:

Lactic acid bacteria

MRS:

de Man, Rogosa and Sharpe

1H NMR:

Proton nuclear magnetic resonance

OD:

Optical density

PEP:

Phosphoenolpyruvate

PK:

Pentose phosphoketolase

PLA:

Polylactic acid

RSM:

Response surface methodology

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Contributions

Jianfei Wang, Shaoming Jiang, and Shijie Liu are the primary contributors of this work. Jiaqi Huang and Huanyu Guo provided important technical support for experiments and manuscript preparation. Xudong Bi, Maolin Hou, Xingyu Chen, Shibo Hou, Hebei Lin, Yuming Lu, Hujie Lv, Jinyue Qiao, and Ruiyi Yang participated in this study and supported in the manuscript preparation.

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Authors and Affiliations

  1. Department of Chemical Engineering, SUNY College of Environmental Science and Forestry, Syracuse, NY, 13210, USA

    Jianfei Wang, Shaoming Jiang, Jiaqi Huang, Huanyu Guo, Xudong Bi, Maolin Hou, Xingyu Chen, Shibo Hou, Hebei Lin, Yuming Lu, Hujie Lv, Jinyue Qiao, Ruiyi Yang & Shijie Liu

  2. The Center for Biotechnology & Interdisciplinary Studies (CBIS) at Rensselaer Polytechnic Institute, Troy, NY, 12180, USA

    Jiaqi Huang

  3. California State University, Los Angeles (CSULA), Los Angeles, CA, 90032, USA

    Xudong Bi

  4. Department of Biomedical Engineering, City University of Hong Kong, Hong Kong, 999077, China

    Maolin Hou

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  1. Jianfei Wang
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Wang, J., Jiang, S., Huang, J. et al. Optimization of Initial Cation Concentrations for L-Lactic Acid Production from Fructose by Lactobacillus pentosus Cells. Appl Biochem Biotechnol 193, 1496–1512 (2021). https://doi.org/10.1007/s12010-021-03492-1

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