Papain (PA) immobilized onto magnetic nanocrystalline cellulose (PA@MNCC) was successfully fabricated and adopted as an efficient biocatalyst for the synthesis of N-(benzyloxycarbonyl)-alanyl-histidine (Z-Ala-His) dipeptide. Introducing deep eutectic solvents (DESs) as reaction media promoted the synthesis of the Z-Ala-His dipeptide. The effects of reaction conditions on the yield of papain catalytic Z-Ala-His were systematically investigated with the highest yield of 68.4%, which was higher than free papain (63.3%). Besides, this novel PA@MNCC composite can be easily recycled from the reaction system by magnetic forces. In a word, the PA@MNCC composite exhibited great potential for efficient biosynthesis of dipeptide in DESs.
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Magnetic nanocrystalline cellulose
Papain immobilized onto magnetic nanocrystalline cellulose
Deep eutectic solvents
N-(Benzyloxycarbonyl)-alanyl methyl ester
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This work was supported by the National Natural Science Foundation of China (21908070, 21676104, 21878105), the National Key Research and Development Program of China (2018YFC1603400, 2018YFC1602100), China Postdoctoral Science Foundation (BX20180102, 2019 M652902), and the Fundamental Research Funds for the Central Universities (2019MS100, 2019PY15).
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Xiong, J., Cao, S., Zong, M. et al. Biosynthesis of Alanyl-Histidine Dipeptide Catalyzed by Papain Immobilized on Magnetic Nanocrystalline Cellulose in Deep Eutectic Solvents. Appl Biochem Biotechnol (2020). https://doi.org/10.1007/s12010-020-03345-3
- Nanocrystalline cellulose
- Dipeptide synthesis
- Deep eutectic solvents