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Application of the Severity Factor and HMF Removal of Red Macroalgae Gracilaria verrucosa to Production of Bioethanol by Pichia stipitis and Kluyveromyces marxianus with Adaptive Evolution

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Abstract

Gracilaria verrucosa, red seaweed, is a promising biomass for bioethanol production due to its high carbohydrate content. The optimal hyper thermal (HT) acid hydrolysis conditions are 12% (w/v) G. verrucosa with 0.2 M H2SO4 at 130 °C for 15 min, with a severity factor of 1.66. This HT acid hydrolysis produces 50.7 g/L monosaccharides. The maximum monosaccharide concentration of 58.0 g/L was achieved with 96.6% of the theoretical monosaccharide production from 120 g dry weight/L G. verrucosa slurry after HT acid hydrolysis and enzymatic saccharification. Fermentation was carried out by removing an inhibitory compound and via yeast adaptation to galactose. Both Pichia stipitis and Kluyveromyces marxianus adapted to galactose were excellent producers, with the ethanol yield (YEtOH) of 0.50 and 29.0 g/L ethanol production. However, the bioethanol productivity with Pichia stipitis adapted to galactose is higher than that with Kluyveromyces marxianus adapted to galactose, being 0.81 and 0.35 g/L/h, respectively. The results from this study can be applied to industrial scale bioethanol production from seaweed.

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References

  1. Jeong, T. S., Choi, C. H., Lee, J. Y., & Oh, K. K. (2012). Behaviors of glucose decomposition during acid-catalyzed hydrothermal hydrolysis of pretreated Gelidium amansii. Bioresource Technology, 116, 435–440.

    Article  CAS  PubMed  Google Scholar 

  2. McHugh, D. J. (2003). A guide to the seaweed industry. Food and Agriculture Organization of the United Nations.

  3. Yanagisawa, M., Kawai, S., & Murata, K. (2013). Strategies for the production of high concentrations of bioethanol from seaweeds: production of high concentrations of bioethanol from seaweeds. Bioengineered, 4(4), 224–235.

    Article  PubMed  PubMed Central  Google Scholar 

  4. Bensah, E. C., & Mensah, M. (2013). Chemical pretreatment methods for the production of cellulosic ethanol: technologies and innovations. International Journal of Chemical Engineering, 2013, 1–21.

    Article  CAS  Google Scholar 

  5. Wyman, C. E., & Yang, B. (2017). Combined severity factor for predicting sugar recovery in acid-catalyzed pretreatment followed by enzymatic hydrolysis. In H. A. Ruiz, M. Thomsen, & H. L. Trajano (Eds.), Hydrothermal processing in biorefineries (pp. 161–180). Cham: Springer International Publishing.

    Chapter  Google Scholar 

  6. Samuel, R., Pu, Y., Foston, M., & Ragauskas, A. J. (2010). Solid-state NMR characterization of switchgrass cellulose after dilute acid pretreatment. Biofuels, 1(1), 85–90.

    Article  CAS  Google Scholar 

  7. Qi, L., Mui, Y. F., Lo, S. W., Lui, M. Y., Akien, G. R., & Horváth, I. T. (2014). Catalytic conversion of fructose, glucose, and sucrose to 5-(hydroxymethyl)furfural and levulinic and formic acids in γ-valerolactone as a green solvent. ACS Catalysis, 4(5), 1470–1477.

    Article  CAS  Google Scholar 

  8. Delgenes, J. P., Moletta, R., & Navarro, J. M. (1996). Effects of lignocellulose degradation products on ethanol fermentations of glucose and xylose by Saccharomyces cerevisiae, Zymomonas mobilis, Pichia stipitis, and Candida shehatae. Enzyme and Microbial Technology, 19(3), 220–225.

    Article  CAS  Google Scholar 

  9. Lenihan, P., Orozco, A., O’Neill, E., Ahmad, M. N. M., Rooney, D. W., & Walker, G. M. (2010). Dilute acid hydrolysis of lignocellulosic biomass. Chemical Engineering Journal, 156(2), 395–403.

    Article  CAS  Google Scholar 

  10. Prakasham, R. S., Rao, R. S., & Hobbs, P. J. (2009). Current trends in biotechnological production of xylitol and future prospects. Current Trends in Biotechnology and Pharmacy, 3(1), 8–36.

    CAS  Google Scholar 

  11. Lane, M. M., & Morrissey, J. P. (2010). Kluyveromyces marxianus: a yeast emerging from its sister’s shadow. Fungal Biology Reviews, 24(1-2), 17–26.

    Article  Google Scholar 

  12. Lopez, C. L. F., Beaufort, S., Brandam, C., & Taillandier, P. (2014). Interactions between Kluyveromyces marxianus and Saccharomyces cerevisiae in tequila must type medium fermentation. World Journal of Microbiology and Biotechnology, 30(8), 2223–2229.

    Article  CAS  PubMed  Google Scholar 

  13. Ra, C. H., Kim, Y. J., Lee, S. Y., Jeong, G.-T., & Kim, S.-K. (2015). Effects of galactose adaptation in yeast for ethanol fermentation from red seaweed, Gracilaria verrucosa. Bioprocess and Biosystems Engineering, 38(9), 1715–1722.

    Article  CAS  PubMed  Google Scholar 

  14. Cho, H., Ra, C. H., & Kim, S.-K. (2014). Ethanol production from the seaweed Gelidium amansii, using specific sugar acclimated yeasts. Journal of Microbiology and Biotechnology, 24(2), 264–269.

    Article  CAS  PubMed  Google Scholar 

  15. Ra, C. H., Choi, J. G., Kang, C.-H., Sunwoo, I. Y., Jeong, G.-T., & Kim, S.-K. (2015). Thermal acid hydrolysis pretreatment, enzymatic saccharification and ethanol fermentation from red seaweed, Gracilaria verrucosa. Microbiology and Biotechnology Letters, 43(1), 9–15.

    Article  CAS  Google Scholar 

  16. Kubicek, C. P. (1982). Beta-glucosidase excretion by Trichoderma pseudokoningii: correlation with cell wall bound beta-1.3-glucanase activities. Archives of Microbiology, 132(4), 349–354.

    Article  CAS  PubMed  Google Scholar 

  17. Mandels, M., Andreotti, R., & Roche, C. (1976). Measurement of saccharifying cellulase. Biotechnology and Bioengineering Symposium, 6, 21–33.

    CAS  Google Scholar 

  18. Fonseca, G. G., Gombert, A. K., Heinzle, E., & Wittmann, C. (2007). Physiology of the yeast Kluyveromyces marxianus during batch and chemostat cultures with glucose as the sole carbon source. FEMS Yeast Research, 7(3), 422–435.

    Article  CAS  PubMed  Google Scholar 

  19. Meinita, M. D. N., Marhaeni, B., Winanto, T., Setyaningsih, D., & Hong, Y.-K. (2015). Catalytic efficiency of sulfuric and hydrochloric acids for the hydrolysis of Gelidium latifolium (Gelidiales, Rhodophyta) in bioethanol production. Journal of Industrial and Engineering Chemistry, 27, 108–114.

    Article  CAS  Google Scholar 

  20. Ra, C. H., Nguyen, T. H., Jeong, G.-T., & Kim, S.-K. (2016). Evaluation of hyper thermal acid hydrolysis of Kappaphycus alvarezii for enhanced bioethanol production. Bio/Technology, 209, 66–72.

    CAS  Google Scholar 

  21. Meinita, M. D. N., Hong, Y.-K., & Jeong, G.-T. (2012). Detoxification of acidic catalyzed hydrolysate of Kappaphycus alvarezii (cottonii). Bioprocess and Biosystems Engineering, 35(1–2), 93–98.

    Article  CAS  PubMed  Google Scholar 

  22. Kim, D.-H., Lee, S.-B., & Jeong, G.-T. (2014). Production of reducing sugar from Enteromorpha intestinalis by hydrothermal and enzymatic hydrolysis. Bioresource Technology, 161, 348–353.

    Article  CAS  PubMed  Google Scholar 

  23. Kwon, O.-M., Kim, D.-H., Kim, S.-K., & Jeong, G.-T. (2016). Production of sugars from macro-algae Gracilaria verrucosa using combined process of citric acid-catalyzed pretreatment and enzymatic hydrolysis. Algal Research, 13, 293–297.

    Article  Google Scholar 

  24. Nguyen, T. H., Ra, C. H., Sunwoo, I. Y., Jeong, G.-T., & Kim, S.-K. (2016). Evaluation of galactose adapted yeasts for bioethanol fermentation from Kappaphycus alvarezii hydrolyzates. Journal of Microbiology and Biotechnology, 26(7), 1259–1266.

    Article  CAS  PubMed  Google Scholar 

  25. Asztalos, A., Daniels, M., Sethi, A., Shen, T., Langan, P., Redondo, A., & Gnanakaran, S. (2012). A coarse-grained model for synergistic action of multiple enzymes on cellulose. Biotechnology for Biofuels, 5(1), 1–15.

    Article  CAS  Google Scholar 

  26. Cannella, D., Hsieh, C. C., Felby, C., & Jørgensen, H. (2012). Production and effect of aldonic acids during enzymatic hydrolysis of lignocellulose at high dry matter content. Biotechnology for Biofuels, 5(1), 1–10.

    Article  CAS  Google Scholar 

  27. Guo, Z., & Olsson, L. (2014). Physiological response of Saccharomyces cerevisiae to weak acids present in lignocellulosic hydrolysate. FEMS Yeast Research, 14(8), 1234–1248.

    Article  CAS  PubMed  Google Scholar 

  28. López-Alvarez, A., Díaz-Pérez, A. L., Sosa-Aguirre, C., Macías-Rodríguez, L., & Campos-García, J. (2012). Ethanol yield and volatile compound content in fermentation of agave must by Kluyveromyces marxianus UMPe-1 comparing with Saccharomyces cerevisiae baker’s yeast used in tequila production. Journal of Bioscience and Bioengineering, 113(5), 614–618.

    Article  PubMed  CAS  Google Scholar 

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Funding

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education (2016R1D1A1A09918683), Korea.

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Author notes

  1. Pailin Sukwong and In Yung Sunwoo contributed equally to this work.

Authors and Affiliations

  1. Department of Biotechnology, Pukyong National University, Busan, 48513, South Korea

    Pailin Sukwong, In Yung Sunwoo, Min Ju Lee, Gwi-Taek Jeong & Sung-Koo Kim

  2. Department of Food Science and Biotechnology, Hankyong National University, Anseong, Gyeonggi, 17579, South Korea

    Chae Hun Ra

Authors
  1. Pailin Sukwong
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  2. In Yung Sunwoo
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  3. Min Ju Lee
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  6. Sung-Koo Kim
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Correspondence to Sung-Koo Kim.

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Sukwong, P., Sunwoo, I.Y., Lee, M.J. et al. Application of the Severity Factor and HMF Removal of Red Macroalgae Gracilaria verrucosa to Production of Bioethanol by Pichia stipitis and Kluyveromyces marxianus with Adaptive Evolution. Appl Biochem Biotechnol 187, 1312–1327 (2019). https://doi.org/10.1007/s12010-018-2888-y

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  • DOI: https://doi.org/10.1007/s12010-018-2888-y

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