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Enhanced Production of Phenolic Compounds in Compact Callus Aggregate Suspension Cultures of Rhodiola imbricata Edgew.

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Abstract

Rhodiola imbricata is a rare medicinal plant of the trans-Himalayan region of Ladakh. It is used for the treatment of numerous health ailments. Compact callus aggregate (CCA) suspension cultures of Rhodiola imbricata were established to counter extinction threats and for production of therapeutically valuable phenolic compounds to meet their increasing industrial demands. The present study also investigated the effect of jasmonic acid (JA) on production of phenolic compounds and bioactivities in CCA suspension cultures. CCA suspension cultures established in an optimized Murashige and Skoog medium supplemented with 30 g/l sucrose, 3 mg/l NAA, and 3 mg/l BAP showed maximum biomass accumulation (8.43 g/l DW) and highest salidroside production (3.37 mg/g DW). Upon 100 μM JA treatment, salidroside production (5.25 mg/g DW), total phenolic content (14.69 mg CHA/g DW), total flavonoid content (4.95 mg RE/g DW), and ascorbic acid content (17.93 mg/g DW) were significantly increased in cultures. In addition, DPPH-scavenging activity (56.32%) and total antioxidant capacity (60.45 mg QE/g DW) were significantly enhanced upon JA treatment, and this was positively correlated with increased accumulation of phenolic compounds. JA-elicited cultures exhibited highest antimicrobial activity against Escherichia coli. This is the first report describing the enhanced production of phenolic compounds and bioactivities from JA-elicited CCA suspension cultures of Rhodiola imbricata.

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Abbreviations

AAC:

Ascorbic acid content

BAP:

6-Benzylaminopurine

CHA:

Chlorogenic acid

DFRSA:

DPPH free radical scavenging activity

DW:

Dry weight

FW:

Fresh weight

JA:

Jasmonic acid

MBC:

Minimal bactericidal concentration

MIC:

Minimal inhibitory concentration

NAA:

1-naphthaleneacetic acid

PGRs:

Plant growth regulators

QE:

Quercetin equivalent

RE:

Rutin equivalent

TAC:

Total antioxidant capacity

TFC:

Total flavonoid content

TPC:

Total phenolic content

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Acknowledgements

The authors wish to acknowledge Defence Research & Development Organization (DRDO), Ministry of Defence, Government of India, for financial support. Authors also wish to acknowledge Dr. Somen Acharya and Sc. ‘C’ Rajkumar for providing necessary facilities. The authors are also grateful to Rashmi Gupta for reviewing and copy-editing of the manuscript.

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Authors and Affiliations

  1. Defence Institute of High Altitude Research (DRDO), C/O 56 APO, Leh-Ladakh, Jammu & Kashmir, 901205, India

    Sahil Kapoor, Ankita Sharma, Pushpender Bhardwaj, Shweta Saxena & Om Prakash Chaurasia

  2. Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat (Solan), Himachal Pradesh, 173215, India

    Hemant Sood

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  1. Sahil Kapoor
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Contributions

Experimental design, analysis and interpretation of data, and writing of the manuscript: SK, AS, HS, SS and OPC; HPLC analysis: SK and PB; Critical revision of the manuscript: HS.

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Correspondence to Hemant Sood.

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Kapoor, S., Sharma, A., Bhardwaj, P. et al. Enhanced Production of Phenolic Compounds in Compact Callus Aggregate Suspension Cultures of Rhodiola imbricata Edgew.. Appl Biochem Biotechnol 187, 817–837 (2019). https://doi.org/10.1007/s12010-018-2851-y

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