Abstract
Lotus seed epicarp, a byproduct of lotus seed production process, is usually discarded as a waste. In this study, antioxidant and anti-α-amylase activities of freeze-dried water and various methanol extracts of lotus seed epicarp were evaluated. The extract obtained by 80% methanol exhibited the strongest DPPH and ABTS radical scavenging activity and ferric reducing power, as well as the greatest inhibitory potential on α-amylase. The excellent antioxidant and α-amylase inhibitory activities of 80% methanol extract might be attributed to its highest concentrations of total phenolics, flavonoids, and condensed tannins. The inhibition kinetic analysis revealed that the 80% methanol extract was a reversible and uncompetitive-type inhibitor of α-amylase. Furthermore, based on MALDI-TOF-MS and thiolysis-HPLC-ESI-MS, the main active components present in 80% methanol extract were identified to be B-type heteropolymeric condensed tannins built up of mixtures of propelargonidins, procyanidins, and prodelphinidins, with the predominance of procyanidins and epicatechin as the main constitutive units. The results obtained suggested that lotus seed epicarp could be exploited as a potential source of natural antioxidants and α-amylase inhibitors.
Similar content being viewed by others
References
Yanishlieva, N. V., Marinova, E., & Pokorny, J. (2006). Natural antioxidants from herbs and spices. European Journal of Lipid Science and Technology, 108(9), 776–793.
Mattei, J., Malik, V., Wedick, N. M., Hu, F. B., Spiegelman, D., Willett, W. C., & Campos, H. (2015). Reducing the global burden of type 2 diabetes by improving the quality of staple foods: the global nutrition and epidemiologic transition initiative. Globalization and Health, 11(1), 23.
Shao, Y. F., & Bao, J. S. (2015). Polyphenols in whole rice grain: genetic diversity and health benefits. Food Chemistry, 180, 86–97.
Tsujita, T., Shintani, T., & Sato, H. (2013). α-Amylase inhibitory activity from nut seed skin polyphenols. 1. Purification and characterization of almond seed skin polyphenols. Journal of Agricultural and Food Chemistry, 61(19), 4570–4576.
Tarling, C. A., Woods, K., Zhang, R., Brastianos, H. C., Brayer, G. D., Andersen, R. J., & Withers, S. G. (2008). The search for novel human pancreatic α-amylase inhibitors: high-throughput screening of terrestrial and marine natural product extracts. Chembiochem, 9(3), 433–438.
Sudha, P., Zinjarde, S. S., Bhargava, S. Y., & Kumar, A. R. (2011). Potent α-amylase inhibitory activity of Indian Ayurvedic medicinal plants. BMC Complementary and Alternative Medicine, 11, 5.
Ranilla, L. G., Kwon, Y. I., Apostolidis, E., & Shetty, K. (2010). Phenolic compounds, antioxidant activity and in vitro inhibitory potential against key enzymes relevant for hyperglycemia and hypertension of commonly used medicinal plants, herbs and spices in Latin America. Bioresource Technology, 101(12), 4676–4689.
Li, Q., Chen, J., Li, T., Liu, C. M., Zhai, Y. X., McClements, D. J., & Liu, J. Y. (2015). Separation and characterization of polyphenolics from underutilized byproducts of fruit production (Choerospondias axillaris peels): inhibitory activity of proanthocyanidins against glycolysis enzymes. Food and Function, 6(12), 3693–3701.
Saeidnia, S., Ara, L., Hajimehdipoor, H., Read, R. W., Arshadi, S., & Nikan, M. (2016). Chemical constituents of Swertia longifolia Boiss. with α-amylase inhibitory activity. Research in Pharmaceutical Sciences, 11(1), 23–32.
Zhang, Y., Wong, A. I. C., Wu, J. E., Karim, N. B. A., & Huang, D. J. (2016). Lepisanthes alata (Malay cherry) leaves are potent inhibitors of starch hydrolases due to proanthocyanidins with high degree of polymerization. Journal of Functional Foods, 25, 568–578.
Wu, J. Z., Zheng, Y. B., Chen, T. Q., Yi, J., Qin, L. P., Rahman, K., & Lin, W. X. (2007). Evaluation of the quality of lotus seed of Nelumbo nucifera Gaertn from outer space mutation. Food Chemistry, 105(2), 540–547.
Chen, X., & Zhou, J. (2011). A study on the chemical composition of lotus seed epicarp. Transactions of the Chinese Society for Agricultural Machinery, 29, 139–141.
Kredy, H. M., Huang, D. H., Xie, B. J., He, H., Yang, E. N., Tian, B. Q., & Xiao, D. (2010). Flavonols of lotus (Nelumbo nucifera, Gaertn.) seed epicarp and their antioxidant potential. European Food Research and Technology, 231(3), 387–394.
Huang, D. H., Hu, C. L., Husam, M. C., Xie, B. J., He, H., & Yang, E. N. (2009). Antioxidant activity and structure of flavonoids from epicarp of Nelumbo nucifera gaertn. Food Science and Technology, 30(23), 209–213.
Chen, S., Fang, L. C., Xi, H. F., Guan, L., Fang, J. B., Liu, Y. L., Wu, B. H., & Li, S. H. (2012). Simultaneous qualitative assessment and quantitative analysis of flavonoids in various tissues of lotus (Nelumbo nucifera) using high performance liquid chromatography coupled with triple quad mass spectrometry. Analytica Chimica Acta, 724(8), 127–135.
Zhou, D. L., Gao, J. H., Yang, H. J., Chen, A. J., & Mai, Z. L. (2011). Analysis on the nutrient components of lotus seed shell and study on the antioxidation activity of flavonoid. Journal of Anhui Agricultural Sciences, 39(7), 3968–3970.
Liu, Y., Ma, S. S., Ibrahim, S. A., Li, E. H., Yang, H., & Huang, W. (2015). Identification and antioxidant properties of polyphenols in lotus seed epicarp at different ripening stages. Food Chemistry, 185 159–164.
Qi, S., & Zhou, D. (2013). Lotus seed epicarp extract as potential antioxidant and anti-obesity additive in Chinese Cantonese sausage. Meat Science, 93(2), 257–262.
Wei, S. D., Lin, Y. M., Liao, M. M., Zhou, H. C., & Li, Y. Y. (2012). Characterization and antioxidative properties of condensed tannins from the mangrove plant Aegiceras corniculatum. Journal of Applied Polymer Science, 124(3), 2463–2472.
Kim, D. O., Jeong, S. W., & Lee, C. Y. (2003). Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chemistry, 81(3), 321–326.
Terrill, T. H., Rowan, A. M., Douglas, G. B., & Barry, T. N. (1992). Determination of extractable and bound condensed tannin concentrations in forage plants, protein concentrate meals and cereal grains. Journal of the Science of Food and Agriculture, 58(3), 321–329.
Wei, S. D., Zhou, H. C., & Lin, Y. M. (2010). Antioxidant activities of extract and fractions from the hypocotyls of the mangrove plant Kandelia candel. International Journal of Molecular Sciences, 11(10), 4080–4093.
Brand-Williams, W., Cuvelier, M. E., & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT- Food Science and Technology, 28(1), 25–30.
Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9-10), 1231–1237.
Fu, C. L., Yang, X. N., Lai, S. J., Liu, C., Huang, S. R., & Yang, H. S. (2015). Structure, antioxidant and α-amylase inhibitory activities of longan pericarp proanthocyanidins. Journal of Functional Foods, 14, 23–32.
Li, C. M., Leverence, R., Trombley, J. D., Xu, S., Yang, J., Tian, Y., Reed, J. D., & Hagerman, A. E. (2010). High molecular weight persimmon (Diospyros kaki L.) proanthocyanidin: a highly galloylated, A-linked tannin with an unusual flavonol terminal unit, myricetin. Journal of Agricultural and Food Chemistry, 58(16), 9033–9042.
Grabber, J. H., Zeller, W. E., & Mueller-Harvey, I. (2013). Acetone enhances the direct analysis of procyanidin- and prodelphinidin-based condensed tannins in lotus species by the butanol-HCl-iron assay. Journal of Agricultural and Food Chemistry, 61(11), 2669–2678.
Schofield, P., Mbugua, D. M., & Pell, A. N. (2001). Analysis of condensed tannins: a review. Animal Feed Science and Technology, 91(1-2), 21–40.
Hummer, W., & Schreier, P. (2008). Analysis of proanthocyanidins. Molecular Nutrition and Food Research, 52(12), 1381–1398.
Wolfe, R. M., Terrill, T. H., & Muir, J. P. (2008). Drying method and origin of standard affect condensed tannin (CT) concentrations in perennial herbaceous legumes using simplified butanol-HCl CT analysis. Journal of the Science of Food and Agriculture, 88(6), 1060–1067.
Singh, P. P., & Chauhan, A. S. M. S. (2009). Activity guided isolation of antioxidants from the leaves of Ricinus communis L. Food Chemistry, 114(3), 1069–1072.
Inglett, G. E., Chen, D. J., Berhow, M., & Lee, S. Y. (2011). Antioxidant activity of commercial buckwheat flours and their free and bound phenolic compositions. Food Chemistry, 125(3), 923–929.
Tan, K. W., & Kassim, M. J. (2011). A correlation study on the phenolic profiles and corrosion inhibition properties of mangrove tannins (Rhizophora apiculata) as affected by extraction solvent. Corrosion Science, 53(2), 569–574.
Wei, S. D., Chen, H., Yan, T., Lin, Y. M., & Zhou, H. C. (2014). Identification of antioxidant components and fatty acid profiles of the leaves and fruits from Averrhoa carambola. LWT- Food Science and Technology, 55(1), 278–285.
Liu, S. C., Lin, J. T., Wang, C. K., Chen, H. Y., & Yang, D. J. (2009). Antioxidant properties of various solvent extracts from lychee (Litchi chinenesis Sonn.) flowers. Food Chemistry, 114(2), 577–581.
Wang, Y. F., Huang, S. R., Shao, S. H., Qian, L. S., & Xu, P. (2012). Studies on bioactivities of tea (Camellia sinensis L.) fruit peel extracts: antioxidant activity and inhibitory potential against glucosidase and amylase in vitro. Industrial Crops and Products, 37(1), 520–526.
Hargrove, J. L., Greenspan, P., Hartle, D. K., & Dowd, C. (2011). Inhibition of aromatase and α-amylase by flavonoids and proanthocyanidins from Sorghum bicolor bran extracts. Journal of Medicinal Food, 14(7-8), 799–807.
Reed, J. D., Krueger, C. G., & Vestling, M. M. (2005). MALDI-TOF mass spectrometry of oligomeric food polyphenols. Phytochemistry, 66(18), 2248–2263.
Wei, S. D., Zhou, H. C., Lin, Y. M., Liao, M. M., & Chai, W. M. (2010). MALDI-TOF MS analysis of condensed tannins with potent antioxidant activity from the leaf, stem bark and root bark of Acacia confusa. Molecules, 15(6), 4369–4381.
Zhou, H. C., Lin, Y. M., Wei, S. D., & Tam, N. F. Y. (2011). Structural diversity and antioxidant activity of condensed tannins fractionated from mangosteen pericarp. Food Chemistry, 129(4), 1710–1720.
Song, W., Zhu, X. F., Ding, X. D., Yang, H. B., Qin, S. T., Chen, H., & Wei, S. D. (2017). Structural features, antioxidant and tyrosinase inhibitory activities of proanthocyanidins in leaves of two tea cultivars. International Journal of Food Properties, 20(6), 1348–1358.
Funding
This work was supported by the National Natural Science Foundation of China (31700360), the Hubei Provincial Scientific Research Project in Environmental Protection (2017HB10), the WEL Visiting Fellowship Program of Xiamen University (WEL201706), the Engineering Research Center of Ecology and Agricultural Use of Wetland of Yangtze University (KF201505, KF201704), and the Yangtze Youth Fund of Yangtze University (2015cqn60, 2016cqn41).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of Interest
The authors declare that they have no conflict of interest.
Rights and permissions
About this article
Cite this article
Chen, H., Sun, K., Yang, Z. et al. Identification of Antioxidant and Anti-α-amylase Components in Lotus (Nelumbo nucifera, Gaertn.) Seed Epicarp. Appl Biochem Biotechnol 187, 677–690 (2019). https://doi.org/10.1007/s12010-018-2844-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12010-018-2844-x