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Genome Sequencing and Analysis of Strains Bacillus sp. AKBS9 and Acinetobacter sp. AKBS16 for Biosurfactant Production and Bioremediation

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Abstract

Microbial genomics facilitates the analysis of microbial attributes, which can be applied in bioremediation of pollutants and oil recovery process. The biosurfactants derived from microbes can replace the chemical surfactants, which are ecologically detrimental. The aim of this work was to study the genetic organization responsible for biodegradation of aromatics and biosurfactant production in potential microbes isolated from polluted soil. Bacterial isolates were tested for biosurfactant production, wherein Bacillus sp. AKBS9 and Acinetobacter sp. AKBS16 exhibited highest biosurfactant production potential. Whole genome sequencing and annotations revealed the occurrence of sfp and NPRS gene in the Bacillibactin biosynthetic gene cluster in AKBS9 strain and emulsan biosynthetic gene cluster in AKBS16 strain for biosurfactant production. Various aromatic compound ring cleaving oxygenases scavenging organic molecules could be annotated for strain AKBS16 using RAST annotations.

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Acknowledgements

The authors acknowledge the Council of Scientific and Industrial Research, India, CSIR-network project ESC-0108-MESER, for supporting this research. Niti B Jadeja (SRF) is grateful to the CSIR. We are also grateful to Director, CSIR-NEERI, Nagpur, for the support. The manuscript has been checked for plagiarism using iThenticate Software under assigned KRC No.: CSIR-NEERI/KRC/2018/JAN/DRC/1.

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Authors and Affiliations

  1. Director Research Cell, National Environmental Engineering Research Institute CSIR, Nehru Marg, Nagpur, 440020, India

    Niti B. Jadeja, Prachiti Moharir & Atya Kapley

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  1. Niti B. Jadeja
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  2. Prachiti Moharir
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  3. Atya Kapley
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Correspondence to Atya Kapley.

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Electronic supplementary material

Supplementary Table 1

Results of confirmatory tests for Biosurfactant production in strains AKBS9 and AKBS16. (DOCX 12 kb)

Supplementary Figure 1

In-silico steps involved in the analysis of AKBS9 and AKBS16 draft genomes. (PNG 171 kb)

High Resolution (TIF 677 kb)

Supplementary Figure 2

Graphical representation of gene ontologies for draft genome assemblies a) AKBS9 and b) AKBS16. (PNG 820 kb)

(PNG 1019 kb)

High Resolution (TIF 3010 kb)

High Resolution (TIF 3457 kb)

Supplementary Figure 3

Circos plot of AKBS9 and closest reference match Bacillus cereusb) Circos plot of AKBS16 with closest reference match Acinetobacter pittii. (PNG 4396 kb)

(PNG 4851 kb)

High Resolution (TIF 6295 kb)

High Resolution (TIF 7273 kb)

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Jadeja, N.B., Moharir, P. & Kapley, A. Genome Sequencing and Analysis of Strains Bacillus sp. AKBS9 and Acinetobacter sp. AKBS16 for Biosurfactant Production and Bioremediation. Appl Biochem Biotechnol 187, 518–530 (2019). https://doi.org/10.1007/s12010-018-2828-x

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  • DOI: https://doi.org/10.1007/s12010-018-2828-x

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