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Enhancing the Production of d-Mannitol by an Artificial Mutant of Penicillium sp. T2-M10

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Abstract

d-Mannitol belongs to a linear polyol with six-carbon and has indispensable usage in medicine and industry. In order to obtain more efficient d-mannitol producer, this study has screened out a stable mutant Penicillium sp. T2-M10 that was isolated from the initial d-mannitol-produced strain Penicillium sp.T2-8 via UV irradiation as well as nitrosoguanidine (NTG) induction. The mutant had a considerable enhancement in yield of d-mannitol based on optimizing fermentation. The production condition was optimized as the PDB medium with 24 g/L glucose for 9 days. The results showed that the production of d-mannitol from the mutant strain T2-M10 increased 125% in contrast with the parental strain. Meanwhile, the fact that d-mannitol is the main product in the mutant simplified the process of purification. Our finding revealed the potential value of the mutant strain Penicillium sp. T2-M10 to be a d-mannitol-producing strain.

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Funding

This work was financially supported by grants from the National Natural Science Foundation of China (No. 81460536 and 81603005), a project of Yunling Scholars of Yunnan province, a grant from the Science and Technology Project of Yunnan Provincial Department of Science and Technology (No. 2017FD059), and a grant from the Scientific Research Foundation of Yunnan Provincial Department of Education (No. 2016zzx003).

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Authors and Affiliations

  1. Key Laboratory of Functional Molecules Analysis and Biotransformation, Yunnan Provincial Department of Education, School of Chemical Science and Technology, Yunnan University, Kunming, 650091, People’s Republic of China

    Rongting Duan, Hongtao Li, Hongyu Li, Linhuan Tang, Hao Zhou, Xueqiong Yang, Yabin Yang & Zhongtao Ding

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  1. Rongting Duan
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  2. Hongtao Li
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  3. Hongyu Li
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  4. Linhuan Tang
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  5. Hao Zhou
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  6. Xueqiong Yang
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  7. Yabin Yang
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  8. Zhongtao Ding
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Correspondence to Hao Zhou or Zhongtao Ding.

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Duan, R., Li, H., Li, H. et al. Enhancing the Production of d-Mannitol by an Artificial Mutant of Penicillium sp. T2-M10. Appl Biochem Biotechnol 186, 990–998 (2018). https://doi.org/10.1007/s12010-018-2791-6

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  • DOI: https://doi.org/10.1007/s12010-018-2791-6

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