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Continuous Laminaribiose Production Using an Immobilized Bienzymatic System in a Packed Bed Reactor

  • Akram Abi
  • Anqi Wang
  • Hans-Joachim Jördening
Article

Abstract

The first continuous production system of laminaribiose from sucrose and glucose in a bienzymatic reaction is reported in this study. Immobilized laminaribiose phosphorylase and sucrose phosphorylase were used in a packed bed reactor system comprising of a 3-cm glass column at 35 °C with a steady feeding flow rate of 0.1 ml/min. Factors affecting product formation including enzyme ratio, peal concept (both enzymes in one pearl or in separate pearls), and pearl size were studied. An enzyme ratio of 2:1 of laminaribiose phosphorylase (LP) to sucrose phosphorylase (SP) when encapsulated separately in bigger size peals resulted in higher concentration of product. Laminaribiose (0.4 g/(L h)) is produced in the optimized system at steady state. The reaction system proved to be operationally stable throughout 10 days of continuous processing. A half-life time of more than 9 days was observed for both biocatalysts.

Keywords

Bienzymatic reaction Continuous process Laminaribiose phosphorylase Packed bed reactor Sucrose phosphorylase 

Abbreviations

BR

Batch reactor

CR

Continuous reactor

GI

Glucose isomerase

Glc

Glucose

Fru

Fructose

G-1-P

Glucose-1-phosphate

HPAEC

High-performance anion exchange chromatography

ISPR

In situ product removal

Lam

Laminaribiose

Lam3

Laminaritriose

LP

Laminaribiose phosphorylase

PBR

Packed bed reactor

SP

Sucrose phosphorylase

Suc

Sucrose

Notes

Acknowledgements

This research was funded by the German Research Foundation (DFG) under grant numbers JO 355/3-2 and SCHO 842/9-2. Authors also would like to thank Bitop company for kind donation of SP enzyme.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Technische Universität BraunschweigBraunschweigGermany

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