Continuous Laminaribiose Production Using an Immobilized Bienzymatic System in a Packed Bed Reactor
The first continuous production system of laminaribiose from sucrose and glucose in a bienzymatic reaction is reported in this study. Immobilized laminaribiose phosphorylase and sucrose phosphorylase were used in a packed bed reactor system comprising of a 3-cm glass column at 35 °C with a steady feeding flow rate of 0.1 ml/min. Factors affecting product formation including enzyme ratio, peal concept (both enzymes in one pearl or in separate pearls), and pearl size were studied. An enzyme ratio of 2:1 of laminaribiose phosphorylase (LP) to sucrose phosphorylase (SP) when encapsulated separately in bigger size peals resulted in higher concentration of product. Laminaribiose (0.4 g/(L h)) is produced in the optimized system at steady state. The reaction system proved to be operationally stable throughout 10 days of continuous processing. A half-life time of more than 9 days was observed for both biocatalysts.
KeywordsBienzymatic reaction Continuous process Laminaribiose phosphorylase Packed bed reactor Sucrose phosphorylase
High-performance anion exchange chromatography
In situ product removal
Packed bed reactor
This research was funded by the German Research Foundation (DFG) under grant numbers JO 355/3-2 and SCHO 842/9-2. Authors also would like to thank Bitop company for kind donation of SP enzyme.
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
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