Analysis of Novel Antioxidant Sesquarterpenes (C35 Terpenes) Produced in Recombinant Corynebacterium glutamicum

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Abstract

Novel synthetic isoprenoids have been synthesized in engineered microbial hosts by evolving terpene synthase or expressing heterologous terpene synthases. Recently, the native operon, crtN a N c M derived from Planococcus sp. PAMC 21323, has isolated for potential industrial applications of C35 carotenoids. For the first time, novel C35 carotenoids (sesquarterpene) were synthesized in Corynebacterium glutamicum expressing the crtN a N c M genes. The recombinant strains accumulate various sesquarterpene including 4-apolycopene (red color), 4-aponeurosporene (yellow color), and no pigmentation, depending on the expression of the genetic elements of the crtN a N c M genes. Subsequently, the carotenoid extract from the cells harboring pCES-H36-CrtNaNcM was analyzed, resulting in significantly higher antioxidant activity than those of other strains harboring pCES-H36-CrtNcM and pCES-H36-CrtNaNc, respectively. This study will promote further engineering of C. glutamicum to increase sesquarterpene productions.

Keywords

CrtMN C35 carotenoids Antioxidant activity Corynebacterium glutamicum 

Notes

Acknowledgements

The authors would liketo thank Dr. Ki Jun Jeong at KAIST for providing a pCES-H36-GFP vector.

Compliance with Ethical Standard

Conflict of Interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Sambandam Ravikumar
    • 1
  • Han Min Woo
    • 2
  • Jong-il Choi
    • 1
  1. 1.Department of Biotechnology and BioengineeringChonnam National UniversityGwangjuSouth Korea
  2. 2.Department of Food Science and BiotechnologySungkyunkwan University (SKKU)SuwonRepublic of Korea

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