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Effect of Enzymatic Digestion of Protein Derivatives Obtained from Mucuna pruriens L. on Production of Proinflammatory Mediators by BALB/c Mouse Macrophages

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Abstract

Inflammation is considered to be a major risk factor for the pathogenesis of chronic non-communicable diseases. Macrophages are important immune cells, which regulate inflammation and host defense by secretion of proinflammatory mediators. Obtaining biopeptides by enzymatic hydrolysis adds value to proteins of vegetative origin, such as Mucuna pruriens L. The present study evaluated the effect of enzymatic digestion of protein derivatives obtained from M. pruriens L. on the production of proinflammatory mediators by BALB/c mouse macrophages. Five different molecular weight peptide fractions were obtained (F > 10, 5–10, 3–5, 1–3, and < 1 kDa, respectively). At 300 μg/mL, F5–10 kDa inhibited 50.26 and 61.00% NO and H2O2 production, respectively. Moreover, F5–10 kDa reduced the IL-6 and TNFα levels to 60.25 and 69.54%, respectively. After enzymatic digestive simulation, F5–10 kDa decreased the inflammatory mediators.

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Acknowledgments

This paper is part of the “Scientific Research Development Directed to develop Protein Derivative of Mucuna pruriens with Potential Biological Activity for Prevention and / or Treatment of Chronic Diseases Associated with Overweight and Obesity” project funded by CONACYT-Mexico (Project 154307).

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Authors and Affiliations

  1. Facultad de Ingeniería Química, Universidad Autónoma de Yucatán, Periférico Norte Km. 33.5, Tablaje Catastral 13615, Col. Chuburná de Hidalgo Inn, 97203, Mérida, Yucatán, Mexico

    Edwin E. Martínez Leo & Maira R. Segura Campos

  2. Facultad de Química, Universidad Autónoma de Yucatán, Calle 43 No. 613 x calle 90 Col. Inalámbrica, 97069, Mérida, Yucatán, Mexico

    Victor E. Arana Argáez

  3. Facultad de Medicina, Universidad Autónoma del Estado de Morelos, Leñeros s/n esq. Iztaccihuatl, Col. Los Volcanes, 62350, Cuernavaca, Morelos, Mexico

    Juan J. Acevedo Fernández

  4. Centro Médico Ignacio García Téllez, Instituto Mexicano del Seguro Social, Unidad de Investigación Médica Yucatán, Unidad Médica de Alta Especialidad, 41 No. 439 x 32 y 34, Col. Industrial, 97150, Mérida, Yucatán, Mexico

    Rosa Moo Puc

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  1. Edwin E. Martínez Leo
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  2. Victor E. Arana Argáez
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  3. Juan J. Acevedo Fernández
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  5. Maira R. Segura Campos
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Correspondence to Maira R. Segura Campos.

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Martínez Leo, E.E., Arana Argáez, V.E., Acevedo Fernández, J.J. et al. Effect of Enzymatic Digestion of Protein Derivatives Obtained from Mucuna pruriens L. on Production of Proinflammatory Mediators by BALB/c Mouse Macrophages. Appl Biochem Biotechnol 186, 597–612 (2018). https://doi.org/10.1007/s12010-018-2740-4

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  • DOI: https://doi.org/10.1007/s12010-018-2740-4

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