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Applied Biochemistry and Biotechnology

, Volume 186, Issue 2, pp 496–506 | Cite as

Efficient Biotransformation of Phytosterols to Dehydroepiandrosterone by Mycobacterium sp.

  • Pei Zhou
  • Ya-kun Fang
  • Hao-ke Yao
  • Hua Li
  • Gang Wang
  • Yu-peng Liu
Article

Abstract

In this study, a method for the efficient production of dehydroepiandrosterone (DHEA) from phytosterols in a vegetable oil/aqueous two-phase system by Mycobacterium sp. was developed. After the 3-hydroxyl group of phytosterols was protected, they could be converted into DHEA with high yield and productivity by Mycobacterium sp. NRRL B-3683. In a shake flask biotransformation, 15.05 g l−1 of DHEA and a DHEA yield of 85.39% (mol mol−1) were attained after 7 days with an initial substrate concentration of 25 g l−1. When biotransformation was carried out in a 30-l stirred bioreactor with 25 g l−1 substrate, the DHEA concentration and yield was 16.33 g l−1 and 92.65% (mol mol−1) after 7 days, respectively. The results of this study suggest that inexpensive phytosterols could be utilized for the efficient production of DHEA.

Keywords

Phytosterols Dehydroepiandrosterone Oil/aqueous two-phase system biotransformation Mycobacterium 

Notes

Funding Information

This research was supported by grants from the Key Project of Science and Technology of Henan Province (No. 152102210255) and the Foundation for University Young Back-bone Teachers of Henan Province (No. 2014GGJS-157).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Life Sciences, Institute of Microbial EngineeringHenan UniversityKaifengPeople’s Republic of China

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