Efficient Biotransformation of Phytosterols to Dehydroepiandrosterone by Mycobacterium sp.
In this study, a method for the efficient production of dehydroepiandrosterone (DHEA) from phytosterols in a vegetable oil/aqueous two-phase system by Mycobacterium sp. was developed. After the 3-hydroxyl group of phytosterols was protected, they could be converted into DHEA with high yield and productivity by Mycobacterium sp. NRRL B-3683. In a shake flask biotransformation, 15.05 g l−1 of DHEA and a DHEA yield of 85.39% (mol mol−1) were attained after 7 days with an initial substrate concentration of 25 g l−1. When biotransformation was carried out in a 30-l stirred bioreactor with 25 g l−1 substrate, the DHEA concentration and yield was 16.33 g l−1 and 92.65% (mol mol−1) after 7 days, respectively. The results of this study suggest that inexpensive phytosterols could be utilized for the efficient production of DHEA.
KeywordsPhytosterols Dehydroepiandrosterone Oil/aqueous two-phase system biotransformation Mycobacterium
This research was supported by grants from the Key Project of Science and Technology of Henan Province (No. 152102210255) and the Foundation for University Young Back-bone Teachers of Henan Province (No. 2014GGJS-157).
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
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