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Detection of Antibodies Against Foot-and-Mouth Disease Virus Serotypes A, O and Asia-1 by ELISA in Strip-Dried Samples from Vaccinated Bovines

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Abstract

Antibodies against foot-and-mouth disease virus serotypes A, O and Asia-1 were detected by ELISA in liquid and strip-dried samples from vaccinated bovines. The results showed high concordance of the results in both types of serum samples (coefficient of correlation varied from 0.89 to 0.96). Stability studies showed that anti-foot-and-mouth disease virus antibodies can be detected in strip-dried serum samples stored at different temperatures on a level of that in native samples. Preliminary study in strip-dried whole blood samples demonstrated good potential of this sample pretreatment procedure for the following anti-foot-and-mouth virus antibodies testing.

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Funding

This work was financially supported by the Ministry of Education and Science of the Russian Federation as a based part of state assignment organization of scientific researches (project No. 16.6548.2017/BY).

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Authors and Affiliations

  1. Faculty of Chemistry, M.V. Lomonosov Moscow State University, Moscow, Russia, 119991

    Jeanne V. Samsonova, Nikolay Yu. Saushkin, Alexander P. Osipov & Sergey E. Kondakov

  2. National University of Science and Technology “MISiS”, Moscow, Russia, 119049

    Jeanne V. Samsonova, Alexander P. Osipov & Sergey E. Kondakov

  3. Federal Centre for Animal Health, Vladimir, Russia, 600901

    Svetlana N. Fomina & Alexey V. Mischenko

Authors
  1. Jeanne V. Samsonova
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  2. Nikolay Yu. Saushkin
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  3. Alexander P. Osipov
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  4. Sergey E. Kondakov
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  5. Svetlana N. Fomina
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  6. Alexey V. Mischenko
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Correspondence to Jeanne V. Samsonova.

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Samsonova, J.V., Saushkin, N.Y., Osipov, A.P. et al. Detection of Antibodies Against Foot-and-Mouth Disease Virus Serotypes A, O and Asia-1 by ELISA in Strip-Dried Samples from Vaccinated Bovines. Appl Biochem Biotechnol 188, 491–497 (2019). https://doi.org/10.1007/s12010-018-02938-3

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  • DOI: https://doi.org/10.1007/s12010-018-02938-3

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