Skip to main content
SpringerLink
Log in

Effects of Heat Treatment on the Structural Characteristics and Antitumor Activity of Polysaccharides from Grifola frondosa

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

This study investigated the effects of heat treatment on structural characteristics and in vitro antitumor activity of polysaccharides from Grifola frondosa. GFP-4 (extracted at 4 °C), GFP-4-80 (80 °C treatment on GFP-4) and GFP-80 (extracted at 80 °C) were prepared, and the chemical composition analysis showed that their total sugar contents were all higher than 90%, high-performance gel-permeation chromatography (HPGPC), ion chromatography (IC) and Fourier-transform infrared spectroscopy (FTIR) results demonstrated that GFP-4 were degraded and denatured after 80 °C heat treatment, MTT and JC-1 results showed that GFP-4 exhibited higher inhibitory effects on HepG2 cells in vitro than GFP-4-80 and GFP-80. Our study suggested that heat treatment at 80 °C on polysaccharides from Grifola frondosa would destroy their structure and attenuate their antitumor effects.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Oliveira, G. K. F., Silva, E. V. d., Ruthes, A. C., Lião, L. M., Iacomini, M., & Carbonero, E. R. (2018). Chemical structure of a partially 3-O-methylated mannofucogalactan from edible mushroom Grifola frondosa. Carbohydrate Polymers, 187, 110–117.

    Article  CAS  PubMed  Google Scholar 

  2. Kou, L., Du, M., Liu, P., Zhang, B., Zhang, Y., Yang, P., Shang, M., & Wang, X. (2018). Anti-diabetic and anti-nephritic activities of Grifola frondosa mycelium polysaccharides in diet-streptozotocin-induced diabetic rats via modulation on oxidative stress. Applied Biochemistry and Biotechnology, 1–13.

  3. Gu, C.-Q., Li, J.-W., Chao, F., Jin, M., Wang, X.-W., & Shen, Z.-Q. (2007). Isolation, identification and function of a novel anti-HSV-1 protein from Grifola frondosa. Antiviral Research, 75(3), 250–257.

    Article  CAS  PubMed  Google Scholar 

  4. Mao, G.-H., Ren, Y., Feng, W.-W., Li, Q., Wu, H.-Y., jin, D., Zhao, T., Xu, C.-Q., Yang, L.-Q., & Wu, X.-Y. (2015). Antitumor and immunomodulatory activity of a water-soluble polysaccharide from Grifola frondosa. Carbohydrate Polymers, 134, 406–412.

    Article  CAS  PubMed  Google Scholar 

  5. Wang, C.-L., Meng, M., Liu, S.-B., Wang, L.-R., Hou, L.-H., & Cao, X.-H. (2013). A chemically sulfated polysaccharide from Grifola frondos induces HepG2 cell apoptosis by notch1–NF-κB pathway. Carbohydrate Polymers, 95(1), 282–287.

    Article  CAS  PubMed  Google Scholar 

  6. He, X., Wang, X., Fang, J., Chang, Y., Ning, N., Guo, H., Huang, L., Huang, X., & Zhao, Z. (2017). Polysaccharides in Grifola frondosa mushroom and their health promoting properties: a review. International Journal of Biological Macromolecules, 101, 910–921.

    Article  CAS  PubMed  Google Scholar 

  7. Liu, A.-J., Yu, J., Ji, H.-Y., Zhang, H.-C., Zhang, Y., & Liu, H.-P. (2018a). Extraction of a novel cold-water-soluble polysaccharide from astragalus membranaceus and its antitumor and immunological activities. Molecules, 23, 62.

    Article  CAS  Google Scholar 

  8. Nurmamat, E., Xiao, H., Zhang, Y., & Jiao, Z. (2018). Effects of different temperatures on the chemical structure and antitumor activities of polysaccharides from cordyceps militaris. Polymers, 10(4), 430.

    Article  CAS  PubMed Central  Google Scholar 

  9. Hromádková, Z., Ebringerová, A., & Valachovic, P. (2002). Ultrasound-assisted extraction of water-soluble polysaccharides from the roots of valerian (Valeriana officinalis L.). Ultrasonics Sonochemistry, 9(1), 37–42.

    Article  PubMed  Google Scholar 

  10. Chen, R.-Z., Tan, L., Jin, C.-G., Lu, J., Tian, L., Chang, Q.-Q., & Wang, K. (2015). Extraction, isolation, characterization and antioxidant activity of polysaccharides from Astragalus membranaceus. Industrial Crops and Products, 77, 434–443.

    Article  CAS  Google Scholar 

  11. Ma, Z.-J., Lu, L., Yang, J.-J., Wang, X.-X., Su, G., Wang, Z.-L., Chen, G.-H., Sun, H.-M., Wang, M.-Y., & Yang, Y. (2018). Lariciresinol induces apoptosis in HepG2 cells via mitochondrial-mediated apoptosis pathway. European Journal of Pharmacology, 821, 1–10.

    Article  CAS  PubMed  Google Scholar 

  12. Ahsani, Z., Mohammadi-Yeganeh, S., Kia, V., Karimkhanloo, H., Zarghami, N., & Paryan, M. (2017). WNT1 gene from WNT signaling pathway is a direct target of miR-122 in hepatocellular carcinoma. Applied Biochemistry and Biotechnology, 181(3), 884–897.

    Article  CAS  PubMed  Google Scholar 

  13. Cui, Y., Lu, P., Song, G., Liu, Q., Zhu, D., & Liu, X. (2016). Involvement of PI3K/Akt, ERK and p38 signaling pathways in emodin-mediated extrinsic and intrinsic human hepatoblastoma cell apoptosis. Food and Chemical Toxicology, 92, 26–37.

    Article  CAS  PubMed  Google Scholar 

  14. Lee, J. S., & Hong, E. K. (2010). Hericium erinaceus enhances doxorubicin-induced apoptosis in human hepatocellular carcinoma cells. Cancer Letters, 297(2), 144–154.

    Article  CAS  PubMed  Google Scholar 

  15. Fitzmorris, P., Shoreibah, M., Anand, B. S., & Singal, A. K. (2015). Management of hepatocellular carcinoma. Journal of Cancer Research and Clinical Oncology, 141(5), 861–876.

    Article  CAS  PubMed  Google Scholar 

  16. Yang, H., Li, J., Cui, L., Ren, Y., Niu, L., Wang, X., Huang, Y., & Cui, L. (2018). Synergistic cytotoxicity and mechanism of caffeine and lysozyme on hepatoma cell line HepG2. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 193, 169–174.

    Article  CAS  Google Scholar 

  17. Silva, A. M., Miranda, A., Fernandes, E., Santos, S., Fraga, I., Santos, D. L., Dias, A. A., & Bezerra, R. M. (2013). Endopolysaccharides from Ganoderma resinaceum, Phlebia rufa, and Trametes versicolor affect differently the proliferation rate of HepG2 cells. Applied Biochemistry and Biotechnology, 169(6), 1919–1926.

    Article  CAS  PubMed  Google Scholar 

  18. Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., & Smith, F. (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28(3), 350–356.

    Article  CAS  Google Scholar 

  19. Li, H., Yu, H., & Zhu, H. (2017). Structure studies of the extracellular polysaccharide from Trichoderma sp. KK19L1 and its antitumor effect via cell cycle arrest and apoptosis. Applied Biochemistry and Biotechnology, 182(1), 128–141.

    Article  CAS  PubMed  Google Scholar 

  20. Yu, J., Ji, H., & Liu, A. (2018a). Preliminary structural characteristics of polysaccharides from pomelo peels and their antitumor mechanism on s180 tumor-bearing mice. Polymers, 10, 419.

    Article  CAS  PubMed Central  Google Scholar 

  21. Wu, H.-T., He, X.-J., Hong, Y.-K., Ma, T., Xu, Y.-P., & Li, H.-H. (2010). Chemical characterization of Lycium barbarum polysaccharides and its inhibition against liver oxidative injury of high-fat mice. International Journal of Biological Macromolecules, 46(5), 540–543.

    Article  CAS  PubMed  Google Scholar 

  22. Xu, P., Yuan, R., Hou, G., Li, J., & Ye, M. (2018). Structural characterization and in vitro antitumor activity of a novel exopolysaccharide from Lachnum YM130. Applied Biochemistry and Biotechnology, 185(2), 541–554.

    Article  CAS  PubMed  Google Scholar 

  23. Zhang, Y., Wu, Y.-T., Zheng, W., Han, X.-X., Jiang, Y.-H., Hu, P.-L., Tang, Z.-X., & Shi, L.-E. (2017). The antibacterial activity and antibacterial mechanism of a polysaccharide from Cordyceps cicadae. Journal of Functional Foods, 38, 273–279.

    Article  CAS  Google Scholar 

  24. Liu, Y., Huang, G., & Hu, J. (2018b). Extraction, characterisation and antioxidant activity of polysaccharides from Chinese watermelon. International Journal of Biological Macromolecules, 111, 1304–1307.

    Article  CAS  PubMed  Google Scholar 

  25. Ma, G., Yang, W., Mariga, A. M., Fang, Y., Ma, N., Pei, F., & Hu, Q. (2014). Purification, characterization and antitumor activity of polysaccharides from Pleurotus eryngii residue. Carbohydrate Polymers, 114, 297–305.

    Article  CAS  PubMed  Google Scholar 

  26. Chen, Y., Xie, M.-Y., Nie, S.-P., Li, C., & Wang, Y.-X. (2008). Purification, composition analysis and antioxidant activity of a polysaccharide from the fruiting bodies of Ganoderma atrum. Food Chemistry, 107(1), 231–241.

    Article  CAS  Google Scholar 

  27. Gong, Y., Zhang, J., Gao, F., Zhou, J., Xiang, Z., Zhou, C., Wan, L., & Chen, J. (2017). Structure features and in vitro hypoglycemic activities of polysaccharides from different species of Maidong. Carbohydrate Polymers, 173, 215–222.

    Article  CAS  PubMed  Google Scholar 

  28. Wang, Y., Wei, X., Wang, F., Xu, J., Tang, X., & Li, N. (2018). Structural characterization and antioxidant activity of polysaccharide from ginger. International Journal of Biological Macromolecules, 111, 862–869.

    Article  CAS  PubMed  Google Scholar 

  29. Yang, L., & Zhang, L.-M. (2009). Chemical structural and chain conformational characterization of some bioactive polysaccharides isolated from natural sources. Carbohydrate Polymers, 76(3), 349–361.

    Article  CAS  Google Scholar 

  30. Pascua-Maestro, R., Corraliza-Gomez, M., Diez-Hermano, S., Perez-Segurado, C., Ganfornina, M. D., & Sanchez, D. (2018). The MTT-formazan assay: complementary technical approaches and in vivo validation in Drosophila larvae. Acta Histochemica, 120(3), 179–186.

    Article  CAS  PubMed  Google Scholar 

  31. Jia, Z., Yang, H. H., Liu, Y.-J., & Wang, X.-Z. (2018). Synthetic dibenzoxanthene derivatives induce apoptosis through mitochondrial pathway in human hepatocellular cancer cells. Applied Biochemistry and Biotechnology, 186(1), 145–160.

    Article  CAS  PubMed  Google Scholar 

  32. Tan, K.-T., Li, S., Li, Y. R., Cheng, S.-L., Lin, S.-H., & Tung, Y.-T. (2018). Synergistic anticancer effect of a combination of paclitaxel and 5-demethylnobiletin against lung cancer cell line in vitro and in vivo. Applied Biochemistry and Biotechnology, 1–16.

  33. Soltan-Dallal, M. M., Validi, M., Douraghi, M., Fallah-Mehrabadi, J., & Lormohammadi, L. (2017). Evaluation the cytotoxic effect of cytotoxin-producing Klebsiella oxytoca isolates on the HEp-2 cell line by MTT assay. Microbial Pathogenesis, 113, 416–420.

    Article  CAS  PubMed  Google Scholar 

  34. Abel, S. D. A., & Baird, S. K. (2018). Honey is cytotoxic towards prostate cancer cells but interacts with the MTT reagent: considerations for the choice of cell viability assay. Food Chemistry, 241, 70–78.

    Article  CAS  PubMed  Google Scholar 

  35. Gelen, V., Şengül, E., Gedikli, S., Atila, G., Uslu, H., & Makav, M. (2017). The protective effect of rutin and quercetin on 5-FU-induced hepatotoxicity in rats. Asian Pacific Journal of Tropical Biomedicine, 7(7), 647–653.

    Article  Google Scholar 

  36. Yassin, A. M., El-Deeb, N. M., Metwaly, A. M., El Fawal, G. F., Radwan, M. M., & Hafez, E. E. (2017). Induction of apoptosis in human cancer cells through extrinsic and intrinsic pathways by Balanites aegyptiaca Furostanol saponins and saponin-coated silver nanoparticles. Applied Biochemistry and Biotechnology, 182(4), 1675–1693.

    Article  CAS  PubMed  Google Scholar 

  37. Faron, J., Bernaś, T., Sas-Nowosielska, H., & Klag, J. (2015). Analysis of the behavior of mitochondria in the ovaries of the earthworm Dendrobaena veneta Rosa 1839. PLoS One, 10(2), e0117187.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  38. Małota, K., Student, S., & Świątek, P. (2018). Low mitochondrial activity within developing earthworm male germ-line cysts revealed by JC-1. Mitochondrion.

  39. Yu, J., Dong, X., Wang, L., Ji, H., & Liu, A. (2018b). Antitumor effects of seleno-β-lactoglobulin (Se-β-Lg) against human gastric cancer MGC-803 cells. European Journal of Pharmacology, 833, 109–115.

    Article  CAS  PubMed  Google Scholar 

  40. Yang, H.-H., Zhang, C., Lai, S.-H., Zeng, C.-C., Liu, Y.-J., & Wang, X.-Z. (2017). Isoliquiritigenin induces cytotoxicity in PC-12 cells in vitro. Applied Biochemistry and Biotechnology, 183(4), 1173–1190.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Street, TEDA, Tianjin, 300457, People’s Republic of China

    Hai-yu Ji, Pei Chen, Juan Yu, Ying-ying Feng & An-jun Liu

  2. QingYunTang Biotech (Beijing) Co., Ltd., Beijing, 100176, China

    Hai-yu Ji, Juan Yu & Ying-ying Feng

Authors
  1. Hai-yu Ji
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Pei Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Juan Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ying-ying Feng
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. An-jun Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to An-jun Liu.

Ethics declarations

Conflicts of Interest

The authors declare that they have no conflicts of interest.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ji, Hy., Chen, P., Yu, J. et al. Effects of Heat Treatment on the Structural Characteristics and Antitumor Activity of Polysaccharides from Grifola frondosa. Appl Biochem Biotechnol 188, 481–490 (2019). https://doi.org/10.1007/s12010-018-02936-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-018-02936-5

Keywords

Search

Navigation