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A Comparative Study of Receptor-Targeted Magnetosome and HSA-Coated Iron Oxide Nanoparticles as MRI Contrast-Enhancing Agent in Animal Cancer Model

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Abstract

Magnetosomes are specialized organelles arranged in intracellular chains in magnetotactic bacteria. The superparamagnetic property of these magnetite crystals provides potential applications as contrast-enhancing agents for magnetic resonance imaging. In this study, we compared two different nanoparticles that are bacterial magnetosome and HSA-coated iron oxide nanoparticles for targeting breast cancer. Both magnetosomes and HSA-coated iron oxide nanoparticles were chemically conjugated to fluorescent-labeled anti-EGFR antibodies. Antibody-conjugated nanoparticles were able to bind the MDA-MB-231 cell line, as assessed by flow cytometry. To compare the cytotoxic effect of nanoparticles, MTT assay was used, and according to the results, HSA-coated iron oxide nanoparticles were less cytotoxic to breast cancer cells than magnetosomes. Magnetosomes were bound with higher rate to breast cancer cells than HSA-coated iron oxide nanoparticles. While 250 μg/ml of magnetosomes was bound 92 ± 0.2%, 250 μg/ml of HSA-coated iron oxide nanoparticles was bound with a rate of 65 ± 5%. In vivo efficiencies of these nanoparticles on breast cancer generated in nude mice were assessed by MRI imaging. Anti-EGFR-modified nanoparticles provide higher resolution images than unmodified nanoparticles. Also, magnetosome with anti-EGFR produced darker image of the tumor tissue in T2-weighted MRI than HSA-coated iron oxide nanoparticles with anti-EGFR. In vivo MR imaging in a mouse breast cancer model shows effective intratumoral distribution of both nanoparticles in the tumor tissue. However, magnetosome demonstrated higher distribution than HSA-coated iron oxide nanoparticles according to fluorescence microscopy evaluation. According to the results of in vitro and in vivo study results, magnetosomes are promising for targeting and therapy applications of the breast cancer cells.

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Abbreviations

BM:

Bacterial magnetosome

HSA:

Human serum albumin

SPIONs:

Superparamagnetic iron oxide nanoparticles

HSA-SPIONs:

Human serum albumin-coated iron oxide nanoparticles

MRI:

Magnetic resonance imaging

Anti-EGFR:

Anti-epidermal growth factor receptor

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Acknowledgements

This study was supported by Hacettepe University. Project number is 014BİYB604002.

The animal study protocol was approved by Animal Research Ethics Committee of Kobay A.Ş. Protocol number 106. 04.03.2014. There is no conflict of interest between authors.

Author information

Authors and Affiliations

  1. Department of Biology, Aksaray University, Aksaray, Turkey

    Ebru Erdal

  2. Advanced Technologies Application and Research Center, Hacettepe University, Beytepe, Ankara, Turkey

    Murat Demirbilek

  3. Department of Bioengineering, University of California at San Diego, La Jolla, CA, USA

    Yasan Yeh

  4. Department of Biology, Polatlı Art and Science Faculty, Gazi University, Polatlı, Ankara, Turkey

    Öznur Akbal

  5. Moores Cancer Center, University of California at San Diego, La Jolla, CA, USA

    Laura Ruff & Sadık Esener

  6. Department of Biology, Osmangazi University, Eskisehir, Turkey

    Damla Bozkurt & Ahmet Cabuk

  7. Biotechnology and Biosafety Division Department, Osmangazi University, Eskisehir, Turkey

    Ahmet Cabuk

  8. Veterinary Internal Medicine Division, Ankara University, Ankara, Turkey

    Yasin Senel

  9. Department of Patology, Yıldırım Beyazıt University, Ataturk Education and Research Hospital, Bilkent, Ankara, Turkey

    Berrak Gumuskaya

  10. Department of Radiology, Yıldırım Beyazıt University, Ataturk Education and Research Hospital, Bilkent, Ankara, Turkey

    Oktay Algın

  11. Physics Engineering Department, Hacettepe University, 06800, Beytepe, Ankara, Turkey

    Seyda Colak

  12. Department of Electrical and Computer Engineering, University of California San Diego, La Jolla, CA, USA

    Sadık Esener

  13. Chemistry Department, Biochemistry Division, Hacettepe University, Beytepe, Ankara, Turkey

    Emir B. Denkbas

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  1. Ebru Erdal
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  2. Murat Demirbilek
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  3. Yasan Yeh
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  4. Öznur Akbal
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  8. Yasin Senel
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  10. Oktay Algın
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  11. Seyda Colak
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  12. Sadık Esener
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  13. Emir B. Denkbas
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Corresponding author

Correspondence to Emir B. Denkbas.

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All methods used in this study were approved by Kobay Laboratory of Experimental Animals Ethics Committee (No:106, 2014).

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Highlights

Biosynthesis and extraction of magnetosome

Synthesis of superparamagnetic iron oxide nanoparticles

Design and development of nanoparticle for targeting to cancer cells

Generated of breast cancer in nude mice

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Erdal, E., Demirbilek, M., Yeh, Y. et al. A Comparative Study of Receptor-Targeted Magnetosome and HSA-Coated Iron Oxide Nanoparticles as MRI Contrast-Enhancing Agent in Animal Cancer Model. Appl Biochem Biotechnol 185, 91–113 (2018). https://doi.org/10.1007/s12010-017-2642-x

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