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Applied Biochemistry and Biotechnology

, Volume 184, Issue 4, pp 1120–1141 | Cite as

Identification and Characterization of Anthocyanin Biosynthesis-Related Genes in Kohlrabi

  • Md Abdur Rahim
  • Arif Hasan Khan Robin
  • Sathishkumar Natarajan
  • Hee-Jeong Jung
  • Jeongyeo Lee
  • HyeRan Kim
  • Hoy-Taek Kim
  • Jong-In Park
  • Ill-Sup Nou
Article

Abstract

Kohlrabi (Brassica oleracea var. gongylodes L.) is an important vegetable of the Brassicaceae family. The main edible part of kohlrabi is the swollen stem. The purple cultivars make anthocyanin mainly in the peel of the swollen stem, while in the leaf, it is limited to the midrib, but green cultivars do not. Anthocyanins are advantageous for both plants as well as humans. Two anthocyanin compounds were detected by high pressure liquid chromatography (HPLC) only in the peel of the purple kohlrabi cultivar. Three MYBs, three bHLHs, and one WD40 TF were identified as the candidate regulatory genes in kohlrabi. There was an abundance of transcript levels for the late biosynthetic genes more specifically for BoF3′H, BoDFR, BoLDOX, and BoGST in the purple peel while scarcely detectable in other tissues for both cultivars. The expression of BoPAP2 and BoTT8 was higher in the peel of the purple cultivar than the green cultivar. The expression of BoMYBL2.2 orthologue of Arabidopsis MYBL2, a negative regulator of anthocyanins, was dramatically decreased in the purple peel. The expression of BoACO1, a key gene for ethylene biosynthesis, and BoNCED3, an important gene of the ABA pathway, was down- and upregulated, respectively, in the peel of purple kohlrabi.

Keywords

Anthocyanin MYB bHLH WD40 repeats Negative regulator Kohlrabi Biosynthetic genes HPLC Ethylene ABA 

Abbreviations

AAO3

arabidopsis aldehyde oxidase 3

ABA

abscisic acid

ACO1

aminocyclopropane-1-carboxylateoxidase 1

bHLH

basic helix-loop-helix

CHI

chalcone isomerase

CHS

chalcone synthase

DFR

dihydroflavonol 4-reductase

EGL3

enhancer of glabra3

ERS

ethylene response sensor

ETR

ethylene receptor

F3H

flavanone 3-hydroxylase

F3′H

flavonoid 3′-hydroxylase

GL3

glabra3

GST

glutathione S-transferase

HPLC

high pressure liquid chromatography

LDOX

leucoanthocyanidin dioxygenase

NCED3

9-cis-epoxycarotenoid dioxygenase 3

PAP1

production of anthocyanin pigment 1

PAP2

production of anthocyanin pigment 1

qRT-PCR

quantitative reverse transcription polymerase chain reaction

TF

transcription factor

TT8

transparent testa8

TTG1

transparent testa glabra 1

UFGT

UDP-flavonoid glucosyl transferase.

Notes

Funding Information

This research work was financially supported by the Golden Seed Project (Center for Horticultural Seed Development, Grant no. 213007-05-1-CG100) of the Ministry of Agriculture, Food and Rural affairs in the Republic of Korea (MAFRA).

Compliance with Ethical Standards

Competing Interests

The authors declare that they have no competing interests.

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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Md Abdur Rahim
    • 1
  • Arif Hasan Khan Robin
    • 1
  • Sathishkumar Natarajan
    • 1
  • Hee-Jeong Jung
    • 1
  • Jeongyeo Lee
    • 2
  • HyeRan Kim
    • 2
  • Hoy-Taek Kim
    • 1
  • Jong-In Park
    • 1
  • Ill-Sup Nou
    • 1
  1. 1.Department of HorticultureSunchon National UniversitySuncheonSouth Korea
  2. 2.Korea Research Institute of Bioscience and BiotechnologyDaejeonSouth Korea

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