Applied Biochemistry and Biotechnology

, Volume 184, Issue 4, pp 1073–1093 | Cite as

Enhancement of Protective Effects of Radix Scutellariae on UVB-induced Photo Damage in Human HaCaT Keratinocytes

  • Yu-shuai Wang
  • Jin-Gyeong Cho
  • Eun-Son Hwang
  • Jung-Eun Yang
  • Wei Gao
  • Min-zhe Fang
  • Sheng-dao Zheng
  • Tae-Hoo Yi


Radix Scutellariae (RS) has long been used in the treatment of inflammatory and allergic diseases. Its main flavonoids, baicalin (BG) and wogonoside (WG), can be hydrolyzed into their corresponding aglycones, baicalein (B) and wogonin (W). In this study, we developed a safe and effective method of transforming these glycosides using Peclyve PR. The transformation rate of BG and WG reached 98.5 and 98.1%, respectively, with 10% enzyme at 40 °C for 60 h. Furthermore, we compared the anti-photoaging activity of RS before and after enzyme treatment, as well as their respective main components, in UVB-irradiated HaCaT cells. Results found that enzyme-treated RS (ERS) appeared to be much better at preventing UVB-induced photoaging than RS. ERS significantly inhibited the upregulation of matrix metalloproteinase-1 and IL-6 caused by UVB radiation by inactivating the MAPK/AP-1 and NF-κB/IκB-α signaling pathways. ERS treatment also recovered UVB-induced reduction of procollagen type I by activating the TGF-β/Smad pathway. In addition, ERS exhibited an excellent antioxidant activity, which could increase the expression of cytoprotective antioxidants such as HO-1 and NQ-O1, by facilitating Nrf2 nuclear transfer. These findings demonstrated that the photoprotective effects of RS were significantly improved by enzyme-modified biotransformation.


Radix Scutellariae Baicalin Wogonoside Peclyve PR Biotransformation Baicalein Wogonin Anti-skin photoaging 



This work was supported by the TAEYI Life Science Co. Ltd., Republic of Korea.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflicts of interest.


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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Yu-shuai Wang
    • 1
  • Jin-Gyeong Cho
    • 1
  • Eun-Son Hwang
    • 1
  • Jung-Eun Yang
    • 1
  • Wei Gao
    • 1
  • Min-zhe Fang
    • 1
  • Sheng-dao Zheng
    • 1
  • Tae-Hoo Yi
    • 1
  1. 1.Department of Oriental Medicinal Material and Processing, College of Life ScienceKyung Hee University Global CampusYongin-siRepublic of Korea

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