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Applied Biochemistry and Biotechnology

, Volume 184, Issue 4, pp 1061–1072 | Cite as

Highly Efficient Deacidification of High-Acid Rice Bran Oil Using Methanol as a Novel Acyl Acceptor

  • Daoming Li
  • Muniba Faiza
  • Shahid Ali
  • Weifei Wang
  • Chin Ping Tan
  • Bo Yang
  • Yonghua Wang
Article
  • 269 Downloads

Abstract

A highly efficient process for reducing the fatty acid (FA) content of high-acid rice bran oil (RBO) was developed by immobilized partial glycerides-selective lipase SMG1-F278N-catalyzed esterification/transesterification using methanol as a novel acyl acceptor. Molecular docking simulation indicated that methanol was much closer to the catalytic serine (Ser-171) compared with ethanol and glycerol, which might be one of the reasons for its high efficiency in the deacidification of high-acid RBO. Additionally, the reaction parameters were optimized to minimize the FA content of high-acid RBO. Under the optimal conditions (substrate molar ratio of methanol to FAs of 1.8:1, enzyme loading of 40 U/g, and at 30 °C), FA content decreased from 25.14 to 0.03% after 6 h of reaction. Immobilized SMG1-F278N exhibited excellent methanol tolerance and retained almost 100% of its initial activity after being used for ten batches. After purification by molecular distillation, the final product contained 97.86% triacylglycerol, 2.10% diacylglycerol, and 0.04% FA. The acid value of the final product was 0.09 mg KOH/g, which reached the grade one standard of edible oil. Overall, methanol was a superior acyl acceptor for the deacidification of high-acid RBO and the high reusability of immobilized SMG1-F278N indicates an economically attractive process.

Keywords

Deacidification High-acid rice bran oil Methanol Molecular docking Partial glycerides-selective lipase 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (21376098) and the Science and Technology Planning Project of Guangdong Province (2014B020204003, 2015B020231006, 2015TX01N207).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Daoming Li
    • 1
  • Muniba Faiza
    • 1
  • Shahid Ali
    • 1
  • Weifei Wang
    • 2
  • Chin Ping Tan
    • 3
  • Bo Yang
    • 4
  • Yonghua Wang
    • 1
  1. 1.School of Food Science and EngineeringSouth China University of TechnologyGuangzhouChina
  2. 2.School of Chemistry and Chemical EngineeringSouth China University of TechnologyGuangzhouChina
  3. 3.Department of Food Technology, Faculty of Food Science and TechnologyUniversiti Putra MalaysiaSerdangMalaysia
  4. 4.School of Bioscience and BioengineeringSouth China University of TechnologyGuangzhouChina

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