Abstract
Rift Valley fever virus (RVFV) is considered an enzootic virus in Africa. RVFV has caused several outbreaks in Egypt, sub-Saharan Africa and the Arabian Peninsula and is responsible for high mortality in ruminants and haemorrhagic fever in severe human cases. Although there are several molecular and serological diagnostic techniques used to detect this arthropod-borne virus with high sensitivity and efficiency, there is a need for a fast and reliable field screening test for rapid outbreak recording and containment. In this study, we developed a prototype point-of-care diagnostic test specific for RVFV detection using unmodified gold nanoparticles (AuNPs) that change colour in the presence of RVFV RNA, resulting in a simple but sensitive assay. The nanogold assay provides qualitative results showing the presence of the RVFV RNA in different sample types. The assay showed high accuracy and specificity, with a detection limit of 10 RNA copies/reaction, comparable with quantitative reverse transcription polymerase chain reaction. The assay result could be determined within 30 min with no need for specific detection instruments. To our knowledge, this is the first field test prototype to directly detect the RNA of RVFV without amplification using AuNPs.
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References
Chevalier, V., Pépin, M., Plée, L., & Lancelot, R. (2010). Rift Valley fever—a threat for Europe? Eurosurveillance Edition, 15(10), 30-40
Elliott, R. M. (1997). Emerging viruses: the Bunyaviridae. Molecular Medicine, 3(9), 572–577.
Daubney, R., Hudson, J. R., & Garnham, P. C. (1931). Enzootic hepatitis or rift valley fever. An undescribed virus disease of sheep cattle and man from East Africa. The Journal of Pathology and Bacteriology, 34(4), 545–579. https://doi.org/10.1002/path.1700340418.
Sayed-Ahmed, M. Z. (2015). Epidemic situation of Rift Valley fever in Egypt and Saudi Arabia. Journal of Dairy, Veterinary & Animal Research, 2(3), 23–25. https://doi.org/10.15406/jdvar.2015.02.00034.
Madani, T. A., Al-Mazrou, Y. Y., Al-Jeffri, M. H., Mishkhas, A. A., Al-Rabeah, A. M., Turkistani, A. M., et al. (2003). Rift Valley fever epidemic in Saudi Arabia: epidemiological, clinical, and laboratory characteristics. Clinical infectious diseases: an official publication of the Infectious Diseases Society of America, 37(8), 1084–1092. https://doi.org/10.1086/378747.
Shaif, A. (2011). The epidemiology of rift valley fever in Yemen and the risk of re-introduction from the horn of Africa. L’épidémiologie de la fièvre de la vallée du Rift au Yemen, PhD thesis, University of Liège. Liège, Belgian
Abdel-Wahab, K. S., El Baz, L. M., El-Tayeb, E. M., Omar, H., Ossman, M. A, & Yasin, W. (1978). Rift Valley Fever virus infections in Egypt: pathological and virological findings in man. Transactions of the Royal Society of Tropical Medicine and Hygiene, 72(4), 392–396. doi:http://dx.doi.org/10.1016/0035-9203(78)90134-7
Ahmed Kamal, S., & Kamal, S. A. (2011). Observations on rift valley fever virus and vaccines in Egypt. Virology Journal, 8, 532. https://doi.org/10.1186/1743-422x-8-532.
Ikegami, T., & Makino, S. (2011). The pathogenesis of rift valley fever. Virus, 3(5), 493–519. https://doi.org/10.3390/v3050493.
Rift valley fever (RVF), transmission. 2013. Avaliable from www.cdc.gov/vhf/rvf/transmission/index.html.
Mansfield, K. L., Banyard, A. C., McElhinney, L., Johnson, N., Horton, D. L., Hernández-Triana, L. M., & Fooks, A. R. (2015). Rift Valley fever virus: a review of diagnosis and vaccination, and implications for emergence in Europe. Vaccine, 33(42), 5520–5531. https://doi.org/10.1016/j.vaccine.2015.08.020.
Adam, A. A., Karsany, M. S., & Adam, I. (2010). Manifestations of severe Rift Valley fever in Sudan. International Journal of Infectious Diseases, 14(2), 179–180. https://doi.org/10.1016/j.ijid.2009.03.029.
Mwaengo, D., Lorenzo, G., Iglesias, J., Warigia, M., Sang, R., Bishop, R. P., & Brun, A. (2012). Detection and identification of Rift Valley fever virus in mosquito vectors by quantitative real-time PCR. Virus Research, 169(1), 137–143. https://doi.org/10.1016/j.virusres.2012.07.019.
Weidmann, M., Sanchez-Seco, M. P., Sall, A. A., Ly, P. O., Thiongane, Y., Lô, M. M., et al. (2008). Rapid detection of important human pathogenic phleboviruses. Journal of Clinical Virology, 41(2), 138–142. https://doi.org/10.1016/j.jcv.2007.10.001.
Niklasson, B., Grandien, M., Peters, C. J., & Gargan, T. P. (1983). Detection of Rift Valley fever virus antigen by enzyme-linked immunosorbent assay. Egypt Journal of Clinical Microbiology, 17(6), 1026–1031.
Peyrefitte, C. N., Boubis, L., Coudrier, D., Bouloy, M., Grandadam, M., Tolou, H. J., & Plumet, S. (2008). Real-time reverse-transcription loop-mediated isothermal amplification for rapid detection of Rift Valley fever virus. Journal of Clinical Microbiology, 46(11), 3653–3659. https://doi.org/10.1128/JCM.01188-08.
Euler, M., Wang, Y., Nentwich, O., Piepenburg, O., Hufert, F. T., & Weidmann, M. (2012). Recombinase polymerase amplification assay for rapid detection of Rift Valley fever virus. Journal of Clinical Virology, 54(4), 308–312. https://doi.org/10.1016/j.jcv.2012.05.006.
Med, I. J., & Res, N. (2016). Nanoparticles: from diagnosis to therapy. International Journal of Medical Nano Research, 3(1), 1–5.
Suri, S. S., Fenniri, H., & Singh, B. (2007). Nanotechnology-based drug delivery systems. Journal of Occupational Medicine and Toxicology, 6, 1–6. https://doi.org/10.1186/1745-6673-2-16.
Radwan, S. H., & Azzazy, H. M. (2009). Gold nanoparticles for molecular diagnostics. Expert Reviews, 9(5), 511–524.
Huang, X., & El-sayed, M. A. (2010). Gold nanoparticles: optical properties and implementations in cancer diagnosis and photothermal therapy. Journal of Advanced Research, 1(1), 13–28. https://doi.org/10.1016/j.jare.2010.02.002.
Shen, X., Jiang, L., Liang, H., Lu, X., Zhang, L., & Liu, X. (2006). Determination of 6-mercaptopurine based on the fluorescence enhancement of Au nanoparticles. Talanta, 69, 456–462. https://doi.org/10.1016/j.talanta.2005.10.017.
Jin, R., Wu, G., Li, Z., Mirkin, C. A., & Schatz, G. C. (2003). What controls the melting properties of DNA-linked gold nanoparticle assemblies? Have been used as probes in a variety of DNA detection methods. Journal of the American Chemical Society, 25, 1643–1654.
Li, H., & Rothberg, L. (2004). Colorimetric detection of DNA sequences based on electrostatic interactions with unmodified gold nanoparticles. Proceeding of the National Academy of Sciences of the United States of America, 101(39), 14036–14039.
Shawky, S. M., Bald, D., & Azzazy, H. M. E. (2010). Direct detection of unamplified hepatitis C virus RNA using unmodified gold nanoparticles. Clinical Biochemistry, 43(13–14), 1163–1168. https://doi.org/10.1016/j.clinbiochem.2010.07.001.
Verma, M. S., Rogowski, J. L., Jones, L., & Gu, F. X. (2015). Colorimetric biosensing of pathogens using gold nanoparticles. Biotechnology Advances. https://doi.org/10.1016/j.biotechadv.2015.03.003.
Eissa, S., Shawky, S. M., Matboli, M., Mohamed, S., & Azzazy, H. M. E. (2014). Direct detection of unamplified hepatoma upregulated protein RNA in urine using gold nanoparticles for bladder cancer diagnosis. Clinical Biochemistry, 47(1–2), 104–110. https://doi.org/10.1016/j.clinbiochem.2013.10.022.
Liu, Y., Zhang, L., Wei, W., Zhao, H., Zhou, Z., Zhang, Y., & Liu, S. (2015). Colorimetric detection of influenza A virus using antibody-functionalized gold nanoparticles. The Analyst, 140(12), 3989–3995. https://doi.org/10.1039/C5AN00407A.
Liu, Y., Wei, M., Zhang, L., Zhang, Y., Wei, W., Yin, L., et al. (2016). Chiroplasmonic assemblies of gold nanoparticles for ultrasensitive detection of 8-hydroxy-2′-deoxyguanosine in human serum sample. Analytical Chemistry, 88(12), 6509–6514. https://doi.org/10.1021/acs.analchem.6b01258.
Eissa, S., Azzazy, H. M. E., Matboli, M., Shawky, S. M., Said, H., & Anous, F. A. (2014). The prognostic value of histidine-rich glycoprotein RNA in breast tissue using unmodified gold nanoparticles assay. Applied Biochemistry and Biotechnology, 174(2), 751–761. https://doi.org/10.1007/s12010-014-1085-x.
Frens, G. (1973). Controlled nucleation for the regulation of the particle size in monodisperse gold suspensions. Nature Physical Science, 241(105), 20–22. https://doi.org/10.1038/physci241020a0.
Balasubramanian, S. K., Yang, L., Yung, L. L., Ong, C., & Ong, W. (2010). Biomaterials characterization, purification and stability of gold nanoparticles. Biomaterials, 31(34), 9023–9030. https://doi.org/10.1016/j.biomaterials.2010.08.012.
Liu, X., Atwater, M., Wang, J., & Huo, Q. (2007). Extinction coefficient of gold nanoparticles with different sizes and different capping ligands. Colloids and Surfaces B: Biointerfaces, 58, 3–7. https://doi.org/10.1016/j.colsurfb.2006.08.005.
Garcia, S., Crance, J. M., Billecocq, A., Peinnequin, A., Jouan, A., Bouloy, M., & Garin, D. (2001). Quantitative real-time PCR detection of Rift Valley fever virus and its application to evaluation of antiviral compounds. Journal of Clinical Microbiology, 39(12), 4456–4461. https://doi.org/10.1128/JCM.39.12.4456.
Liu, Z., Xia, X., Yang, C., & Wang, L. (2015). Visual detection of maize chlorotic mottle virus using unmodified gold nanoparticles. RSC Advances, 5, 100891–100897. https://doi.org/10.1039/C5RA16326A.
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We thank Dr. James Allen from the AuthorAid organisation for extensive paper editing and language proofreading.
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Zaher, M.R., Ahmed, H.A., Hamada, K.E.Z. et al. Colorimetric Detection of Unamplified Rift Valley Fever Virus Genetic Material Using Unmodified Gold Nanoparticles. Appl Biochem Biotechnol 184, 898–908 (2018). https://doi.org/10.1007/s12010-017-2592-3
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DOI: https://doi.org/10.1007/s12010-017-2592-3