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Single Cell Oil Production from Hydrolysates of Inulin by a Newly Isolated Yeast Papiliotrema laurentii AM113 for Biodiesel Making

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Abstract

Microbial oils are among the most attractive alternative feedstocks for biodiesel production. In this study, a newly isolated yeast strain, AM113 of Papiliotrema laurentii, was identified as a potential lipid producer, which could accumulate a large amount of intracellular lipids from hydrolysates of inulin. P. laurentii AM113 was able to produce 54.6% (w/w) of intracellular oil in its cells and 18.2 g/l of dry cell mass in a fed-batch fermentation. The yields of lipid and biomass were 0.14 and 0.25 g per gram of consumed sugar, respectively. The lipid productivity was 0.092 g of oil per hour. Compositions of the fatty acids produced were C14:0 (0.9%), C16:0 (10.8%), C16:1 (9.7%), C18:0 (6.5%), C18:1 (60.3%), and C18:2 (11.8%). Biodiesel obtained from the extracted lipids could be burnt well. This study not only provides a promising candidate for single cell oil production, but will also probably facilitate more efficient biodiesel production.

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Acknowledgements

This work was supported by the Project of Shandong Province Higher Educational Science and Technology Program (J15LE15), Research Foundation for Advanced Talents of Qingdao Agricultural University (6631114335), Petrochemical Joint Fund of National Natural Science Foundation of China (U1462109), and the Taishan Scholar Construction Foundation of Shandong Province (6631114314).

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Authors and Affiliations

  1. College of Life Sciences, Shandong Province Key Laboratory of Applied Mycology, Qingdao Agricultural University, Qingdao, 266109, China

    Guangyuan Wang & Lin Liu

  2. College of Agronomy and Plant Protection, the Key Laboratory of Integrated Crop Pest Management of Shandong Province, Qingdao Agricultural University, No. 700 Changcheng Road, Qingdao, 266109, China

    Wenxing Liang

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  1. Guangyuan Wang
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  2. Lin Liu
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Correspondence to Guangyuan Wang or Wenxing Liang.

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Wang, G., Liu, L. & Liang, W. Single Cell Oil Production from Hydrolysates of Inulin by a Newly Isolated Yeast Papiliotrema laurentii AM113 for Biodiesel Making. Appl Biochem Biotechnol 184, 168–181 (2018). https://doi.org/10.1007/s12010-017-2538-9

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