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CO2 Removal from Biogas by Cyanobacterium Leptolyngbya sp. CChF1 Isolated from the Lake Chapala, Mexico: Optimization of the Temperature and Light Intensity

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Abstract

In the present study, the capacity of the cyanobacterium Leptolyngbya sp. CChF1 to remove CO2 from real and synthetic biogas was evaluated. The identification of the cyanobacterium, isolated from the lake Chapala, was carried out by means of morphological and molecular analyses, while its potential for CO2 removal from biogas streams was evaluated by kinetic experiments and optimized by a central composite design coupled to a response surface methodology. Results demonstrated that Leptolyngbya sp. CChF1 is able to remove CO2 and grow indistinctly in real or synthetic biogas streams, showing tolerance to high concentrations of CO2 and CH4, 25 and 75%, respectively. The characterization of the biomass composition at the end of the kinetic assays revealed that the main accumulated by-products under both biogas streams were lipids, followed by proteins and carbohydrates. Regarding the optimization experiments, light intensity and temperature were the studied variables, while synthetic biogas was the carbon source. Results showed that light intensity was significant for CO2 capture efficiency (p = 0.0290), while temperature was significant for biomass production (p = 0.0024). The predicted CO2 capture efficiency under optimal conditions (27.1 °C and 920 lx) was 93.48%. Overall, the results of the present study suggest that Leptolyngbya sp. CChF1 is a suitable candidate for biogas upgrading.

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Acknowledgments

We thank the Fondo Sectorial CONACYT-SENER-Sustentabilidad Energética, Clúster Biocombustibles Gaseosos project 247006 for financial support. Francisco J. Choix acknowledges CONACYT for the support under the Cátedras CONACYT project 2517. Finally, the technical support provided by Dra. Rocio Gonzalez-Castañeda and Dr. Fernando Jáuregui-Huerta from the Neurosciences laboratory (microscopy) of CUCS—Universidad de Guadalajara is also acknowledged.

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Authors and Affiliations

  1. Departamento de Ingeniería Química, CUCEI—Universidad de Guadalajara, Blvd. M. García Barragán 1421, 44430, Guadalajara, JAL, Mexico

    Francisco J. Choix, Raúl Snell-Castro, Jorge Arreola-Vargas, Alberto Carbajal-López & Hugo O. Méndez-Acosta

  2. CONACYT Research Fellow, CUCEI—Universidad de Guadalajara, Blvd. M. García Barragán 1421, 44430, Guadalajara, JAL, Mexico

    Francisco J. Choix

  3. División de Procesos Industriales, Universidad Tecnológica de Jalisco, Luis J. Jiménez 577-1 de Mayo, 44979, Guadalajara, JAL, Mexico

    Jorge Arreola-Vargas

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  1. Francisco J. Choix
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Correspondence to Hugo O. Méndez-Acosta.

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Choix, F.J., Snell-Castro, R., Arreola-Vargas, J. et al. CO2 Removal from Biogas by Cyanobacterium Leptolyngbya sp. CChF1 Isolated from the Lake Chapala, Mexico: Optimization of the Temperature and Light Intensity. Appl Biochem Biotechnol 183, 1304–1322 (2017). https://doi.org/10.1007/s12010-017-2499-z

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