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Optimization of Inexpensive Agricultural By-Products as Raw Materials for Bacitracin Production in Bacillus licheniformis DW2

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Abstract

Bacitracin is a broad-spectrum antibiotic used extensively as a feed additive. In this study, inexpensive agricultural by-products were used as nitrogen sources for bacitracin production. Based on both the orthogonal tests, a combination of 7% soybean meal (SBM) +2% low protein rapeseed cake (LPRC) was optimal for bacitracin production. Compared to the original formula, the titer of bacitracin increased by 20.5% reaching 910.4 U/ml in flasks. The titer of bacitracin and the ratio of bacitracin A increased by 12.4 and 6.8% in a 50-l fermentor. Furthermore, this study also explored the effects of exogenously adding different amino acids on the yield of bacitracin. The addition of Cys and Glu enhanced bacitracin production by 5.7 and 5.0%, respectively. This study provided the inexpensive nutrient inputs into efficient bacitracin production and also the insight to further research enabling better utilization of oil cakes for economic viability of the bioprocess industry.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (Grand No: 2015CFB214), the National Natural Science Foundation of Hubei Province of China (Grand No: 31500074), the Applied Basic Research Program of Wuhan Science and Technology Bureau (Grand No: 2016060101010062), and the Opening Project of Hubei Key Laboratory of Lipid Chemistry and Nutrition.

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Authors and Affiliations

  1. Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, Wuhan, 430062, China

    Qin Wang, Junhui Li, Christopher T. Nomura & Shouwen Chen

  2. State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China

    Hui Zheng & Shouwen Chen

  3. Oilcrops Research Institute, Chinese Academy of Agricultural Science, Wuhan, 430062, China

    Xia Wan & Hongfeng Huang

  4. Department of Chemistry, The State University of New York College of Environmental Science and Forestry (SUNY-ESF), Syracuse, NY, 13210, USA

    Christopher T. Nomura

  5. Tobacco Research Institute of Hubei Province, Wuhan, 430062, China

    Changjun Wang

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  1. Qin Wang
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  2. Hui Zheng
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  3. Xia Wan
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  4. Hongfeng Huang
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  7. Changjun Wang
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Correspondence to Changjun Wang or Shouwen Chen.

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Wang, Q., Zheng, H., Wan, X. et al. Optimization of Inexpensive Agricultural By-Products as Raw Materials for Bacitracin Production in Bacillus licheniformis DW2. Appl Biochem Biotechnol 183, 1146–1157 (2017). https://doi.org/10.1007/s12010-017-2489-1

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  • DOI: https://doi.org/10.1007/s12010-017-2489-1

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