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A Method for Microalgae Proteomics Analysis Based on Modified Filter-Aided Sample Preparation

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Abstract

With the fast development of microalgal biofuel researches, the proteomics studies of microalgae increased quickly. A filter-aided sample preparation (FASP) method is widely used proteomics sample preparation method since 2009. Here, a method of microalgae proteomics analysis based on modified filter-aided sample preparation (mFASP) was described to meet the characteristics of microalgae cells and eliminate the error caused by over-alkylation. Using Chlamydomonas reinhardtii as the model, the prepared sample was tested by standard LC-MS/MS and compared with the previous reports. The results showed mFASP is suitable for most of occasions of microalgae proteomics studies.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 31470432) and Provincial Natural Science Foundation of Liaoning (No. 2014020090). The authors also want to express their thanks to Dr. Qin Hong Qing’s constructive discussions on the setting of the mass spectrum parameters.

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Authors and Affiliations

  1. School of Bioengineering, Dalian Polytechnic University, Dalian, 116034, China

    Song Li, Zhen Zhu, Haowei Zhang & Jing Tian

  2. Marine Bioengineering Group, Dalian Institute of Chemical Physics, CAS, Dalian, 116023, China

    Xupeng Cao & Song Xue

  3. Novel Technology and Materials for Separation and Detection of Biomolecules Group, Dalian Institute of Chemical Physics, CAS, Dalian, 116023, China

    Yan Wang

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  1. Song Li
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  2. Xupeng Cao
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  3. Yan Wang
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  4. Zhen Zhu
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  5. Haowei Zhang
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  6. Song Xue
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  7. Jing Tian
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Correspondence to Xupeng Cao, Song Xue or Jing Tian.

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Li, S., Cao, X., Wang, Y. et al. A Method for Microalgae Proteomics Analysis Based on Modified Filter-Aided Sample Preparation. Appl Biochem Biotechnol 183, 923–930 (2017). https://doi.org/10.1007/s12010-017-2473-9

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  • DOI: https://doi.org/10.1007/s12010-017-2473-9

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