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Enhanced Production of Poly-γ-glutamic Acid by Bacillus licheniformis TISTR 1010 with Environmental Controls

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Abstract

Bacillus licheniformis TISTR 1010 was used for glutamic acid-independent production of poly-γ-glutamic acid (γ-PGA). A fed-batch production strategy was developed involving feedings of glucose, citric acid, and ammonium chloride at specified stages of the fermentation. With the dissolved oxygen concentration controlled at ≥50% of air saturation and the pH controlled at ~7.4, the fed-batch operation at 37 °C afforded a peak γ-PGA concentration of 39.9 ± 0.3 g L−1 with a productivity of 0.926 ± 0.006 g L−1 h−1. The observed productivity was nearly threefold greater than previously reported for glutamic acid-independent production using the strain TISTR 1010. The molecular weight of γ-PGA was in the approximate range of 60 to 135 kDa.

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Acknowledgements

This research was supported by the Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, and the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission.

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Authors and Affiliations

  1. Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, 50 Ngam Wong Wan Road, Ladyaow, Chatuchak, Bangkok, 10900, Thailand

    Nuttawut Kongklom & Sarote Sirisansaneeyakul

  2. Center for Advanced Studies in Tropical Natural Resources, National Research University-Kasetsart University, Kasetsart University, Chatuchak, Bangkok, 10900, Thailand

    Nuttawut Kongklom & Sarote Sirisansaneeyakul

  3. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China

    Zhongping Shi

  4. School of Engineering, Massey University, Private Bag 11 222, Palmerston North, New Zealand

    Yusuf Chisti

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  1. Nuttawut Kongklom
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  4. Sarote Sirisansaneeyakul
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Correspondence to Sarote Sirisansaneeyakul.

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Kongklom, N., Shi, Z., Chisti, Y. et al. Enhanced Production of Poly-γ-glutamic Acid by Bacillus licheniformis TISTR 1010 with Environmental Controls. Appl Biochem Biotechnol 182, 990–999 (2017). https://doi.org/10.1007/s12010-016-2376-1

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  • DOI: https://doi.org/10.1007/s12010-016-2376-1

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