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Nitrosative Stress Response in Vibrio cholerae: Role of S-Nitrosoglutathione Reductase

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Abstract

Vibrio cholerae, the causative agent of cholera, poses serious threats to humans worldwide. V. cholerae faces host inflammatory response and encounters nitrosative stress before establishing successful colonization. It is not clear how V. cholerae combats nitric oxide and reactive nitrogen species. In the present study, we used three clinical strains of V. cholerae and tested their nitrosative stress response pattern towards sodium nitroprusside (SNP) and S-Nitrosoglutathione (GSNO). Among them, V. cholerae, belonging to both O1 and O139 serotypes, showed moderate resistance to SNP and GSNO. However, a V. cholerae strain belonging to non O1 and non O139 showed sensitivity to SNP but resistance towards GSNO. Reduced glutathione and glutathione reductase play a significant role to combat nitrosative stress in V. cholerae. This is the first report where we show the presence of GSNO reductase activity in V. cholerae and that it plays an important role to detoxify S-Nitrosoglutathione. GSNO reductase activity of V. cholerae was regulated by posttranslational modification through S-nitrosylation under in vitro conditions which could be reversed by dithiothreitol (DTT). In addition, we show that biofilm formation remained unaffected under nitrosative stress in V. cholerae.

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Acknowledgements

We thank DBT, DBT-IPLS, UPE, and UGC CAS Phase II Government of India, CU-CRNN for providing infrastructural facility, DBT, Government of India for providing fellowship to Sourav Kumar Patra. We thank Mr. Chinmay Saha for his assistance in preparing the sequence alignment.

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  1. Department of Biochemistry, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, West Bengal, 700019, India

    Sourav Kumar Patra, Prasanta Kumar Bag & Sanjay Ghosh

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  1. Sourav Kumar Patra
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  2. Prasanta Kumar Bag
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Correspondence to Sanjay Ghosh.

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Patra, S.K., Bag, P.K. & Ghosh, S. Nitrosative Stress Response in Vibrio cholerae: Role of S-Nitrosoglutathione Reductase. Appl Biochem Biotechnol 182, 871–884 (2017). https://doi.org/10.1007/s12010-016-2367-2

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