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Structure Characterization and Lead Detoxification Effect of Carboxymethylated Melanin Derived from Lachnum Sp.

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Abstract

In the present study, an intracellular melanin, named LIM205, was separated from Lachnum YM205 mycelia and was purified on a Sephadex G-15 column. The molecular weight of LIM205 was determined as 522 Da, and its molecular formula was speculated as C28H14N2O7S. The possible chemical structure of LIM205 was determined according to the results of Fourier transform infrared (FT-IR), 1H NMR, 13C NMR, and pyrolysis/GC-MS analysis. With the aim to increase its water solubility, its carboxymethylated derivative, named CLIM205, was formed by the substitution of hydrogen atoms in LIM205 with one, two, and three carboxymethylate groups. FT-IR, UV, and ESI-MS analysis demonstrated that the carboxymethylate groups were conjugated onto LIM205. The lead detoxification activities of LIM205 and CLIM205 had also been investigated. In vivo test showed that both LIM205 and CLIM205 reduced the tissue lead concentration, enhanced lead excretion, and reversed lead-induced alterations in superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) concentrations in mice, with CLIM205 showed better efficacy. The study indicates that LIM205 and CLIM205 have significant lead detoxification effect which will contribute to solve related problems.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (31270060).

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Authors and Affiliations

  1. College of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, China

    Shuai Zong, Lan Li, Jinglei Li, Farnaz Shaikh, Liu Yang & Ming Ye

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  1. Shuai Zong
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  2. Lan Li
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  5. Liu Yang
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Correspondence to Jinglei Li or Ming Ye.

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All animals were treated in accordance with the guidelines of the Principle of Laboratory Animal Care (NIH Publication, revised 1985)

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Shuai Zong and Lan Li contributed equally to this work.

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Zong, S., Li, L., Li, J. et al. Structure Characterization and Lead Detoxification Effect of Carboxymethylated Melanin Derived from Lachnum Sp.. Appl Biochem Biotechnol 182, 669–686 (2017). https://doi.org/10.1007/s12010-016-2353-8

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