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Multiresidue Screening of Veterinary Drugs in Meat, Milk, Egg, and Fish Using Liquid Chromatography Coupled with Ion Trap Time-of-Flight Mass Spectrometry

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Abstract

New approaches to veterinary drug screening based on liquid chromatography-mass spectrometry (LC-MS/MS) and time-of-flight mass spectrometry (ToF/MS) are rapid and have high selectivity and sensitivity. In this study, we developed a multiresidue method for screening over 100 veterinary drug residues using ion trap (IT)-ToF/MS. The screened compounds comprised major drug classes used in veterinary practice, representing the following: amphenicols, anthelmintics, benzimidazoles, β-lactams, coccidiostats, ionophores, macrolides, non-steroidal anti-inflammatory drugs, quinolones, sulfonamides, tetracyclines, and tranquilizers. The method was developed based on chromatographic retention time, specific accurate mass, isotope distribution, and fragment data. Each compound was validated at three levels, and the mass accuracy, accuracy, and repeatability were calculated. All parameters showed acceptable values and conformed to the Commission Decision 2002/657/EC criteria. This screening method can simultaneously analyze over 100 veterinary drugs in meat, milk, eggs, and fish in a single analytical run.

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Acknowledgements

This study was supported by veterinary science research project grants from the Korean Animal and Plant Quarantine Agency.

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Authors and Affiliations

  1. Veterinary Drugs and Biologics Division, Animal and Plant Quarantine and Inspection Agency, Gyeonggi-do, 430-757, Republic of Korea

    JeongWoo Kang, Hae-Chul Park & Md Akil Hossain

  2. Ministry of Food and Drug Safety, Osong Health Technology Administration Complex, Chungcheongbuk-do, 363-700, Republic of Korea

    Su-Jeong Park, Myeong-Ae Kim, Seong-Wan Son, Chae-Mi Lim & Byung-Hoon Cho

  3. Department of Physiology, College of Veterinary Medicine, Kyungpook National University, Daegu, 702-701, Republic of Korea

    Tae-Wan Kim

Authors
  1. JeongWoo Kang
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  2. Su-Jeong Park
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  3. Hae-Chul Park
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  4. Md Akil Hossain
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  5. Myeong-Ae Kim
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Corresponding authors

Correspondence to Tae-Wan Kim or Byung-Hoon Cho.

Additional information

JeongWoo Kang and Su-Jeong Park contributed equally to this study.

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Kang, J., Park, SJ., Park, HC. et al. Multiresidue Screening of Veterinary Drugs in Meat, Milk, Egg, and Fish Using Liquid Chromatography Coupled with Ion Trap Time-of-Flight Mass Spectrometry. Appl Biochem Biotechnol 182, 635–652 (2017). https://doi.org/10.1007/s12010-016-2350-y

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  • DOI: https://doi.org/10.1007/s12010-016-2350-y

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