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Collagen Hydrolysates of Skin Shavings Prepared by Enzymatic Hydrolysis as a Natural Flocculant and Their Flocculating Property

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Abstract

A series of collagen hydrolysates (CHs) were prepared from pigskin shavings by using pepsin (PCH), trypsin (TCH), Alcalase (ACH), HCl (HCH), and NaOH (NCH). Their physicochemical properties, including degree of collagen hydrolysis, molecular weight distribution, electric charge, and microstructure, were investigated, and their flocculation performance was evaluated in a kaolin suspension, at varied pHs and concentrations. PCH exhibited high flocculation capability under acidic and neutral conditions, and its efficiency for removing suspended particles was approximately 80% at a concentration of 0.05 g/L. TCH, ACH, HCH, and NCH showed almost no flocculation capability. The flocculation capability of PCH could be mainly due to a combination of optimal molecular weight distribution and electric charge. This study could provide an environment-friendly natural flocculant and also proposes a promising approach for the reuse of collagen wastes.

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Acknowledgements

This research was financially supported by the Specialized Research Fund for the Doctoral Program of Higher Education of China (no. 20130181120094), the National Natural Science Foundation of China (no. 31601442, no. 31371775), and the Science and Technology Support Program of Sichuan Province (no. 2016NZ0007).

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Authors and Affiliations

  1. Department of Food Engineering, Sichuan University, Chengdu, 610065, People’s Republic of China

    Ruijie Fu, Kai Yao, Dongying Jia, Jiayuan Zhao & Yuanlong Chi

  2. Sichuan Academy of Food and Fermentation Industries, Chengdu, 611130, People’s Republic of China

    Qisheng Zhang

  3. Chengdu Food and Drug Administration Research Institute, Chengdu, 610041, People’s Republic of China

    Yuanlong Chi

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  1. Ruijie Fu
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  2. Kai Yao
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  4. Dongying Jia
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Correspondence to Yuanlong Chi.

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Fu, R., Yao, K., Zhang, Q. et al. Collagen Hydrolysates of Skin Shavings Prepared by Enzymatic Hydrolysis as a Natural Flocculant and Their Flocculating Property. Appl Biochem Biotechnol 182, 55–66 (2017). https://doi.org/10.1007/s12010-016-2310-6

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