Abstract
Lignocellulolytic enzyme activities of selective fungi Daedalea flavida MTCC 145 (DF-2), Phlebia radiata MTCC 2791 (PR), and non-selective fungus Flavodon flavus MTCC 168 (FF) were studied for pretreatment of cotton stalks. Simultaneous productions of high LiP and laccase activities by DF-2 during early phase of growth were effective for lignin degradation 27.83 ± 1.25 % (w/w of lignin) in 20-day pretreatment. Production of high MnP activity without laccase in the early growth phase of PR was ineffective and delayed lignin degradation 24.93 ± 1.53 % in 25 days due to laccase production at later phase. With no LiP activity, low activities of MnP and laccase by FF yielded poor lignin degradation 15.09 ± 0.6 % in 20 days. Xylanase was predominant cellulolytic enzyme produced by DF-2, resulting hemicellulose as main carbon and energy source with 83 % of cellulose recovery after 40 days of pretreatment. The glucose yield improved more than two fold from 20-day DF-2 pretreated cotton stalks after enzymatic saccharification.
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Acknowledgments
Mr. Harmanpreet Meehnian gratefully acknowledges Ministry of Human Resource Development (MHRD), Government of India, for providing the fellowship during the study. All authors are highly thankful to National Institute of Technology (NIT), Jalandhar, for providing grants and administrative supports for the study.
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Meehnian, H., Jana, A.K. Cotton Stalk Pretreatment Using Daedalea flavida, Phlebia radiata, and Flavodon flavus: Lignin Degradation, Cellulose Recovery, and Enzymatic Saccharification. Appl Biochem Biotechnol 181, 1465–1484 (2017). https://doi.org/10.1007/s12010-016-2296-0
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DOI: https://doi.org/10.1007/s12010-016-2296-0