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Use of Bacillus amyloliquefaciens HZ-12 for High-Level Production of the Blood Glucose Lowering Compound, 1-Deoxynojirimycin (DNJ), and Nutraceutical Enriched Soybeans via Fermentation

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Abstract

1-Deoxynojirimycin (DNJ) is an efficient α-glucosidase inhibitor (α-GI) with potential applications in the prevention and treatment of diabetes. In this study, 16 Bacillus strains were screened for α-GI rate, and the strain HZ-12 with the highest α-GI rate was identified as Bacillus amyloliquefaciens through the analysis of physiological biochemical characteristics and 16S rDNA sequence. By LC-MS/Q-TOF analysis, the α-GI component produced by B. amyloliquefaciens HZ-12 was identified as DNJ. Soybean was used as the substrate for the solid-state fermentation; 870 mg/kg DNJ was produced by B. amyloliquefaciens HZ-12 after optimizing the fermentation conditions and media, which was 3.83-fold higher than the initial yield. Also, evaluations of nutraceutical enrichment in the form of anticoagulant activity, antioxidant activity, total nitrogen (TN), and total reducing sugars (TRS) of the B. amyloliquefaciens HZ-12 fermented soybeans were substantially higher than unfermented soybeans. This study provided a promising strain for high-level production of DNJ and produced nutraceutical enriched soybeans by fermentation.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31501468), the National Science & Technology Pillar Program during the Twelfth Five-Year Plan Period (2013AA102801-52), and the Opening Project of State Key Laboratory of Agricultural Microbiology (AMLKF201403).

Authors’ Contributions

X Wei and S Chen designed the study. D Cai, M Liu, and X Li carried out the experiments for this study. D Cai, X Wei, Q Wang, C.T. Nomura, and S Chen analyzed the data and wrote the manuscript. All authors read and approved the final manuscript.

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Authors and Affiliations

  1. Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, No. 368 Youyi Avenue, Wuchang District, Wuhan, 430062, Hubei, China

    Dongbo Cai, Qin Wang, Christopher T. Nomura & Shouwen Chen

  2. State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

    Dongbo Cai, Mengjie Liu, Xuetuan Wei, Xinmiao Li & Shouwen Chen

  3. College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

    Xuetuan Wei

  4. Department of Chemistry, The State University of New York College of Environmental Science and Forestry (SUNY ESF), Syracuse, NY, 13210, USA

    Christopher T. Nomura

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  1. Dongbo Cai
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  2. Mengjie Liu
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Correspondence to Shouwen Chen.

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Cai, D., Liu, M., Wei, X. et al. Use of Bacillus amyloliquefaciens HZ-12 for High-Level Production of the Blood Glucose Lowering Compound, 1-Deoxynojirimycin (DNJ), and Nutraceutical Enriched Soybeans via Fermentation. Appl Biochem Biotechnol 181, 1108–1122 (2017). https://doi.org/10.1007/s12010-016-2272-8

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