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Expression and Enzyme Activity Detection of a Sepiapterin Reductase Gene from Musca domestica Larva

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Abstract

Tetrahydrobiopterin (BH4) is an essential cofactor for aromatic acid hydroxylases and nitric oxide synthase. Sepiapterin reductase (SPR) catalyzes the final steps of BH4 biosynthesis. Studies on SPR from several insects and other organisms have been reported. However, thus far, enzyme activity of SPR in Musca domestica is kept unknown. In this study, 186 differentially expressed genes including SPR gene from Musca domestica (MDSPR) were screened in subtractive cDNA library. The MDSPR gene was cloned, and the recombinant MDSPI16 protein was expressed as a 51-kDa protein in soluble form. The MDSPR exhibited strong activity to the substrate sepiapterin (SP). The values of Vmax and Km of the MDSPR for SP were 6.83 μM/min and 23.48 μM, and the optimum temperature and pH of MDSPR were 50 °C and 4.0, respectively. This study provides new hypotheses and methods for the production of BH4 using insect-derived SPR.

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Acknowledgments

This study was funded by the National Natural Science Foundation of China (Grant Nos. 31572574 and 31502121).

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Authors and Affiliations

  1. College of Animal Science and Technology, Jilin Agricultural University, Xincheng Street No.2888, Changchun, 130118, China

    Yan Tang, Zhihua Pei, Lei Liu, Lingcong Kong, Shuming Liu, Xiuyun Jiang, Yunhang Gao & Hongxia Ma

  2. Jilin Medical University, Jilin Street No.5, Jilin, Jilin Province, 132013, China

    Lei Liu

  3. Chengdu tongwei Co. Ltd,houwu district, Chengdu, Sichuan Province, 610041, China

    Dongfang Wang

Authors
  1. Yan Tang
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  2. Zhihua Pei
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  3. Lei Liu
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  6. Shuming Liu
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Correspondence to Hongxia Ma.

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Yan Tang and Zhihua Pei contributed equally to this work and should be considered co-first authors.

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Tang, Y., Pei, Z., Liu, L. et al. Expression and Enzyme Activity Detection of a Sepiapterin Reductase Gene from Musca domestica Larva. Appl Biochem Biotechnol 181, 604–612 (2017). https://doi.org/10.1007/s12010-016-2235-0

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  • DOI: https://doi.org/10.1007/s12010-016-2235-0

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