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Genipin Cross-Linked Glucose Oxidase and Catalase Multi-enzyme for Gluconic Acid Synthesis

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Abstract

In this work, glucose oxidase (GOD) and catalase (CAT) were used simultaneously to produce gluconic acid from glucose. In order to reduce the distance between the two enzymes, and therefore improve efficiency, GOD and CAT were cross-linked together using genipin. Improvements in gluconic acid production were due to quick removal of harmful intermediate hydrogen peroxide by CAT. GOD activity was significantly affected by the proportion of CAT in the system, with GOD activity in the cross-linked multi-enzyme (CLME) being 10 times higher than that in an un-cross-linked GOD/CAT mixture. The glucose conversion rate after 15 h using 15 % glucose was also 10 % higher using the CLME than was measured using a GOD/CAT mixture.

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Acknowledgments

This work was supported by the National High-Tech R&D Program of China (863 Program) (2013AA020302), and the National Nature Science Foundation of China (21576019, 21436002).

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Authors and Affiliations

  1. National Energy R&D Center for Biorefinery, Beijing Key Laboratory of Bioprocess, College of Biology Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People’s Republic of China

    Caixia Cui, Haibin Chen, Biqiang Chen & Tianwei Tan

Authors
  1. Caixia Cui
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  2. Haibin Chen
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  3. Biqiang Chen
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  4. Tianwei Tan
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Corresponding author

Correspondence to Biqiang Chen.

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Research Highlights

• A simple, efficient cross-linking method for multi-enzymes was developed.

• Genipin cross-linked GOD and CAT (CLME) significantly influences GOD activity.

• GOD activity saw a 10-fold increase when cross-linked with CAT by genipin.

• The CLME exhibited a higher catalytic efficiency for glucose conversion.

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Cui, C., Chen, H., Chen, B. et al. Genipin Cross-Linked Glucose Oxidase and Catalase Multi-enzyme for Gluconic Acid Synthesis. Appl Biochem Biotechnol 181, 526–535 (2017). https://doi.org/10.1007/s12010-016-2228-z

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  • DOI: https://doi.org/10.1007/s12010-016-2228-z

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