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Thermophilic Dry Methane Fermentation of Distillation Residue Eluted from Ethanol Fermentation of Kitchen Waste and Dynamics of Microbial Communities

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Abstract

Thermophilic dry methane fermentation is advantageous for feedstock with high solid content. Distillation residue with 65.1 % moisture content was eluted from ethanol fermentation of kitchen waste and subjected to thermophilic dry methane fermentation, after adjusting the moisture content to 75 %. The effect of carbon to nitrogen (C/N) ratio on thermophilic dry methane fermentation was investigated. Results showed that thermophilic dry methane fermentation could not be stably performed for >10 weeks at a C/N ratio of 12.6 and a volatile total solid (VTS) loading rate of 1 g/kg sludge/d; however, it was stably performed at a C/N ratio of 19.8 and a VTS loading rate of 3 g/kg sludge/d with 83.4 % energy recovery efficiency. Quantitative PCR analysis revealed that the number of bacteria and archaea decreased by two orders of magnitude at a C/N ratio of 12.6, whereas they were not influenced at a C/N ratio of 19.8. Microbial community analysis revealed that the relative abundance of protein-degrading bacteria increased and that of organic acid-oxidizing bacteria and acetic acid-oxidizing bacteria decreased at a C/N ratio of 12.6. Therefore, there was accumulation of NH4 + and acetic acid, which inhibited thermophilic dry methane fermentation.

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Authors and Affiliations

  1. College of Architecture and Environment, Sichuan University, No. 24 South Section 1, First Ring Road, Chengdu, 610065, China

    Yu-Lian Huang, Li Tan, Ting-Ting Wang, Zhao-Yong Sun, Yue-Qin Tang & Kenji Kida

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  1. Yu-Lian Huang
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  2. Li Tan
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  3. Ting-Ting Wang
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Correspondence to Kenji Kida.

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Huang, YL., Tan, L., Wang, TT. et al. Thermophilic Dry Methane Fermentation of Distillation Residue Eluted from Ethanol Fermentation of Kitchen Waste and Dynamics of Microbial Communities. Appl Biochem Biotechnol 181, 125–141 (2017). https://doi.org/10.1007/s12010-016-2203-8

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