Abstract
Cells of Candida guilliermondii permeabilized with Triton X-100 were able to efficiently produce xylitol from a medium composed only by d-xylose and MgCl2·6H2O in potassium phosphate buffer, at 35 °C and pH 6.5. Under these conditions, the results were similar to those obtained when cofactor and co-substrate or nutrients were added to the medium (about 95 % d-xylose was assimilated producing 42 g/L of xylitol, corresponding to 0.80 g/g yield and 2.65 g/L h volumetric productivity). Furthermore, the permeabilized cells kept the d-xylose assimilation in about 90 % and the xylitol production in approx. 40 g/L during three bioconversion cycles of 16 h each. These values are highly relevant when compared to others reported in the literature using enzyme technology and fermentative process, thereby demonstrating the effectiveness of the proposed method. The present study reveals that the use of permeabilized cells is an interesting alternative to obtain high xylitol productivity using low cost medium formulation. This approach may allow the future development of xylitol production from xylose present in lignocellulosic biomass, with additional potential for implementation in biorefinery strategies.
Similar content being viewed by others
References
Mussatto, S. I., & Roberto, I. C. (2002). Xilitol: Edulcorante com efeitos benéficos para a saúde humana. Brazilian Journal of Pharmaceutical Sciences, 38, 401–413.
Mussatto, S. I. (2012). Application of xylitol in food formulations and benefits for health. In S. S. da Silva & A. K. Chandel (Eds.), D-xylitol. Fermentative production, fermentation and commercialization (pp. 309–323). Berlin Heidelberg: Springer-Verlag.
Schlosser, S., & Blahušiak, M. (2011). Biorefinery for production of chemicals, energy and fuels. Elektroenergetika, 4, 8–16.
Prakasham, R. S., Rao, R. S., & Hobbs, P. J. (2009). Current trends in biotechnological production of xylitol and future prospects. Current Trends in Biotechnology and Pharmacy, 3, 8–36.
Almeida, J. R. M., Fávaro, L. C. L., & Quirino, B. F. (2012). Biodiesel biorefinery: opportunities and challenges for microbial production of fuels and chemicals from glycerol waste. Biotechnology for Biofuels, 5, 48.
Winkelhausen, E., & Kuzmanova, S. (1998). Microbial conversion of D-xylose to xylitol. Journal of Fermentation and Bioengineering, 86, 1–14.
Gurpilhares, D. B., Pessoa, A., Jr., & Roberto, I. C. (2006). Glucose-6-phosphate dehydrogenase and xylitol production by Candida guilliermondii FTI 20037 using statistical experimental design. Process Biochemistry, 41, 631–637.
Nidetzky, B., Neuhauser, W., Haltrich, D., & Kulbe, K. D. (1996). Continuous enzymatic production of xylitol with simultaneous coenzyme regeneration in a charged membrane reactor. Biotechnology and Bioengineering, 52, 387–396.
Cánovas, M., & Iborra, J. L. (2005). Whole cell biocatalysts stabilization for L-carnitine production. Biocatalysis and Biotransformation, 23, 149–158.
Abraham, J., & Bhat, S. G. (2008). Permeabilization of baker’s yeast with N-lauroyl sarcosine. Journal of Industrial Microbiology and Biotechnology, 35, 799–804.
Marzzoco, A. & Torres, B.B. (1990). In: Bioquímica Básica. Editora Guanabara, Rio de Janeiro, p. 40–58.
Cornish-Bowden, A. (2012). Fundamentals of enzyme kinetics (4th ed.). Weinheim: Wiley-Blackwel.
Cortez, D. V., & Roberto, I. C. (2012). CTAB, Triton X-100 and freezing–thawing treatments of Candida guilliermondii: effects on permeability and accessibility of the glucose-6-phosphate dehydrogenase, xylose reductase and xylitol dehydrogenase enzymes. New Biotechnology, 29, 192–198.
Cortez, D. V., & Roberto, I. C. (2014). Optimization of D-xylose to xylitol biotransformation by Candida guilliermondii cells permeabilized with Triton X-100. Biocatalysis and Biotransformation, 32, 34–38.
Le, H. D., Thanonkeo, P., & Le, V. V. M. (2013). Impact of high temperature on ethanol fermentation by Kluyveromyces marxianus immobilized on banana leaf sheath pieces. Applied Biochemistry and Biotechnology, 171, 806–816.
Sampaio, F. C., Mantovani, H. C., Passos, F. J. V., Moraes, C. A., Converti, A., & Passos, F. M. L. (2005). Bioconversion of D-xylose to xylitol by Debaryomyces hansenii UFV-170: product formation versus growth. Process Biochemistry, 40, 3600–3606.
Domínguez, J. M. (1998). Xylitol production by free and immobilized Debaryomyces hansenii. Biotechnology Letters, 20, 53–56.
Acknowledgments
This work was financially supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) of Brazil (Proc. 302675/2013-2 and Proc. 509291/2010-4).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Cortez, D.V., Mussatto, S.I. & Roberto, I.C. Improvement on d-xylose to Xylitol Biotransformation by Candida guilliermondii Using Cells Permeabilized with Triton X-100 and Selected Process Conditions. Appl Biochem Biotechnol 180, 969–979 (2016). https://doi.org/10.1007/s12010-016-2146-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12010-016-2146-0