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Effect of Plant Growth Promoting Bacteria Associated with Halophytic Weed (Psoralea corylifolia L) on Germination and Seedling Growth of Wheat Under Saline Conditions

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Abstract

Halotolerant bacteria associated with Psoralea corylifolia L., a luxuriantly growing annual weed in salinity-affected semi-arid regions of western Maharashtra, India were evaluated for their plant growth-promoting activity in wheat. A total of 79 bacteria associated with different parts viz., root, shoot and nodule endophytes, rhizosphere, rhizoplane, and leaf epiphytes, were isolated and grouped based on their habitat. Twelve bacteria isolated for their potential in plant growth promotion were further selected for in vitro studies. Molecular identification showed the presence of the genera Bacillus, Pantoea, Marinobacterium, Acinetobacter, Enterobacter, Pseudomonas, Rhizobium, and Sinorhizobium (LC027447-53; LC027455; LC027457, LC027459, and LC128410). The phylogenetic studies along with carbon source utilization profiles using the Biolog® indicated the presence of novel species and the in planta studies revealed promising results under salinity stress. Whereas the nodule endophytes had minute plant growth-promoting (PGP) activity, the cell free culture filtrates of these strains enhanced seed germination of wheat (Triticum aestivum L). The maximum vigor index was monitored in isolate Y7 (Enterobacter sp strain NIASMVII). Indole acetic acid (IAA) production by the isolates ranged between 0.22 and 25.58 μg mL−1. This signifies the need of exploration of their individual metabolites for developing next-generation bio-inoculants through co-inoculation with other compatible microbes. This study has potential in utilization of the weed-associated microbiome in terms of alleviation of salinity stress in crop plants.

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Acknowledgements

Financial assistance from Indian Council of Agricultural Research (ICAR), Govt. of India under Application of Microorganisms in Agriculture and Allied Sectors (AMAAS) scheme is gratefully acknowledged.

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Authors and Affiliations

  1. School of Edaphic Stress Management, ICAR-National Institute of Abiotic Stress Management, Baramati, Pune, 413115, Maharashtra, India

    Ajay M. Sorty, Kamlesh K. Meena, Khushboo Choudhary, Utkarsh M. Bitla, P. S. Minhas & K. K. Krishnani

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  1. Ajay M. Sorty
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  2. Kamlesh K. Meena
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  3. Khushboo Choudhary
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  4. Utkarsh M. Bitla
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Correspondence to Kamlesh K. Meena.

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Highlights

Weed, Psoralea corylifolia L., growing in salinity-affected semi-arid regions, was explored for its associated plant growth-promoting bacteria.

The isolates exhibited potent plant growth-promoting properties in vitro. Culture filtrates containing metabolites significantly promoted seed germination in wheat under high salt environment.

This study has potential in exploitation of weed-associated microbes for development of salt stress resilient bio-inoculant in crop plants

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Sorty, A.M., Meena, K.K., Choudhary, K. et al. Effect of Plant Growth Promoting Bacteria Associated with Halophytic Weed (Psoralea corylifolia L) on Germination and Seedling Growth of Wheat Under Saline Conditions. Appl Biochem Biotechnol 180, 872–882 (2016). https://doi.org/10.1007/s12010-016-2139-z

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  • DOI: https://doi.org/10.1007/s12010-016-2139-z

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